Results 31 to 40 of about 1,209 (243)

Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]

Journal of Pharmaceutical Sciences, 2021
We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund, Nikolai, Lorenzen, Christian, Rischel   +2 more
openaire   +2 more sources

Asymmetrical Flow Field-Flow Fractionation Coupled to ICP-MS for Characterization of Trace Metal Species in the Environment from Macromolecular to Nano-Assemblage Forms: Current Challenges for Quantification

CHIMIA, 2022
Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior.
Isabelle A. M. Worms, Vera I. Slaveykova
doaj   +1 more source

Comparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4) Channels for Nanoparticle Separations

Separations, 2017
The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system.
Zengchao You, Florian Meier, Steffen Weidner   +2 more
doaj   +1 more source

Size and optical properties of dissolved organic matter in large boreal rivers during mixing: Implications for carbon transport and source discrimination

Journal of Hydrology: Regional Studies, 2022
Study region: The lower Athabasca River (LAR), Northern Alberta, Canada Study focus: The functionality of dissolved organic matter (DOM) in natural waters largely depends upon its size and composition.
Jin-Ping Xue, Chad W. Cuss, Tommy Noernberg, Muhammad B. Javed, Na Chen, Rick Pelletier, Yu Wang, William Shotyk   +7 more
doaj   +1 more source

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage ϕ6 [PDF]

Journal of Chromatography B, 2018
Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies. Advances in the production steps have shifted the bottle neck of the process to the purification. Nonetheless, the development of purification techniques
Lampi, Mirka, Oksanen, Hanna M., Meier, Florian, Moldenhauer, Evelin, Poranen, Minna M., Bamford, Dennis H., Eskelin, Katri   +6 more
openaire   +3 more sources

Quality control and purification of ready-to-use conjugated gold nanoparticles to ensure effectiveness in biosensing

Frontiers in Sensors, 2022
Introduction: Gold nanoparticles (AuNPs) and their conjugates are used for many applications in the field of sensors. Literature lacks procedures able to separate, purify and characterize these species in native conditions without altering them while ...
Junjie Wang, Stefano Giordani, Valentina Marassi, Valentina Marassi, Barbara Roda, Barbara Roda, Pierluigi Reschiglian, Pierluigi Reschiglian, Andrea Zattoni, Andrea Zattoni   +9 more
doaj   +1 more source

First Evidence of Acyl-Hydrolase/Lipase Activity From Human Probiotic Bacteria: Lactobacillus rhamnosus GG and Bifidobacterium longum NCC 2705

Frontiers in Microbiology, 2020
Lactobacillus rhamnosus GG (ATCC 53103) and Bifidobacterium longum NCC 2705 are among the most studied probiotics. However, the first evidence of acyl hydrolase/lipase of two annotated proteins, one in each genome of these strains, is reported in this ...
Panagiotis Manasian, Panagiotis Manasian, Atma-Sol Bustos, Atma-Sol Bustos, Björn Pålsson, Andreas Håkansson, J. Mauricio Peñarrieta, Lars Nilsson, Javier A. Linares-Pastén   +8 more
doaj   +1 more source

Phosphatidylinositol Stabilizes Fluid-Phase Liposomes Loaded with a Melphalan Lipophilic Prodrug

Pharmaceutics, 2021
Previously, a liposomal formulation of a chemotherapeutic agent melphalan (Mlph) incorporated in a fluid lipid bilayer of natural phospholipids in the form of dioleoylglyceride ester (MlphDG) was developed and the antitumor effect was confirmed in mouse ...
Daria Tretiakova, Irina Le-Deigen, Natalia Onishchenko, Judith Kuntsche, Elena Kudryashova, Elena Vodovozova   +5 more
doaj   +1 more source

Marine Exopolysaccharide Complexed With Scandium Aimed as Theranostic Agents

Molecules, 2021
(1) Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc.
Mattia Mazza, Cyrille Alliot, Corinne Sinquin, Sylvia Colliec-Jouault, Pascal E. Reiller, Sandrine Huclier-Markai   +5 more
doaj   +1 more source

A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation

Applied Sciences, 2020
Conjugates of gold nanoparticles (GNPs) and antibodies are widely used in various fields of biochemistry and microbiology. However, the procedure for obtaining such conjugates remains precarious, and the properties of conjugates differ significantly for ...
Dmitriy V. Sotnikov, Irina V. Safenkova, Anatoly V. Zherdev, Vadim G. Avdienko, Irina V. Kozlova, Suren S. Babayan, Vladislav Ya. Gergert, Boris B. Dzantiev   +7 more
doaj   +1 more source