Results 21 to 30 of about 1,209 (243)

A One‐Step Workflow for Size‐Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection [PDF]

Journal of Extracellular Biology
Extracellular vesicles (EVs) are released by diverse cell types in biofluids and are increasingly studied in liquid biopsies as diagnostic and prognostic biomarkers for various diseases, including cancer.
Lien Lippens, Niké Guilbert, Sofie Van Dorpe, Sarah Deville, Robin Boiy, Quentin Roux, Nicolaas Lumen, Ilkka Miinalainen, Pekka Rappu, Katrien Vandecasteele, Hannelore Denys, Bruno De Geest, Olivier De Wever, An Hendrix   +13 more
doaj   +2 more sources

Physicochemical characterization and study of molar mass of industrial gelatins by AsFlFFF-UV/MALS and chemometric approach. [PDF]

PLoS ONE, 2018
Industrial gelatins have different physicochemical properties that mainly depend of the raw materials origin and the extraction conditions. These properties are closely related to the molar mass distribution of these gelatins.
Simon Duthen, Chloé Rochat, Didier Kleiber, Frederic Violleau, Jean Daydé, Christine Raynaud, Cecile Levasseur-Garcia   +6 more
doaj   +1 more source

Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]

Journal of Separation Science, 2009
AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld, Dominik, Ausbacher, Martin, Brandl   +2 more
openaire   +2 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4)

Current Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang, Mats Leeman, Alejandra Castro, Erik Börjesson, Lars Nilsson   +4 more
doaj   +1 more source

Revision of the calibration experiment in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2021
Asymmetrical flow field-flow fractionation is a versatile chromatographic fractionation method. In combination with at least one detection technique it is used for size-based separation of colloids, biomolecules and polymers. Although often used as pure separation method, a well-elaborated theory is available that allows precise quantification of the ...
Häusele, Benedikt, Gindele, Maxim Benjamin, Cölfen, Helmut   +2 more
openaire   +3 more sources

Asymmetric flow field flow fractionation methods for virus purification [PDF]

Journal of Chromatography A, 2016
Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna, Lampi, Mirka, Meier, Florian, Moldenhauer, Evelin, Bamford, Dennis Henry, Oksanen, Hanna Maarit   +5 more
openaire   +3 more sources

Aqueous Dilution of Noble NPs Bulk Dispersions: Modeling Instability due to Dissolution by AF4 and Stablishing Considerations for Plasmonic Assays

Nanomaterials, 2020
Among different nanomaterials, gold and silver nanoparticles (AuNPs and AgNPs) have become useful tools for a wide variety of applications in general, and particularly for plasmonic assays.
Lorenzo Sanjuan-Navarro, Aaron Boughbina-Portolés, Yolanda Moliner-Martínez, Pilar Campíns-Falcó   +3 more
doaj   +1 more source

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

Molecules, 2023
Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian, Nemal Gobalasingham, Anatolii Purchel, Jessica Lin   +3 more
doaj   +1 more source

Field-Flow Fractionation in Molecular Biology and Biotechnology

Molecules, 2023
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension.
Stefano Giordani, Valentina Marassi, Anna Placci, Andrea Zattoni, Barbara Roda, Pierluigi Reschiglian   +5 more
doaj   +1 more source

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension [PDF]

Journal of Visualized Experiments, 2020
Particle size is arguably the most important physico-chemical parameter associated with the notion of a nanoparticle. Precise knowledge of the size and size distribution of nanoparticles is of utmost importance for various applications. The size range is also important, as it defines the most "active" component of a nanoparticle dose. Asymmetrical Flow
Drexel, Roland, Sogne, Vanessa, Dinkel, Magdalena, Meier, Florian, Klein, Thorsten   +4 more
openaire   +2 more sources