Results 261 to 270 of about 26,804,949 (344)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Subnanometer control and electrochemical mapping of a Pt monolayer catalyst for boosting ethanol oxidation. [PDF]

Sci Adv
Zhu JW, Zhang M, Wang JC, Zhou XX, Pan ZY, Zhong J, Liang QM, Shi J, Han L, Zhan D, Huang X, Yang Y.   +11 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Integrating in situ single-particle cryo-electron microscopy with cryo-electron tomography for high-resolution structural biology in native cellular contexts. [PDF]

Curr Opin Struct Biol
Yue J, Guo W, Liu J.
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Atomic-scale mechanism of anisotropic ion migration in 2D Bi₂O₂Se nanodevices. [PDF]

Nat Commun
Qu K, Zhang J, Chen J, Yao C, Zeng J, Wang H, Xin T, Chen WT, Chu YH, Liu P, Huang R, Cheng Y, Yang Z, Yang H, Duan C, Gao P.   +15 more
europepmc   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

ZrCo₃B₂ and HfCo₃B₂ Electrodes Go on Stage for Oxygen Evolution Reaction. [PDF]

Adv Sci (Weinh)
Aras F, Burkhardt U, Altendorf SG, Grin Y, Antonyshyn I.   +4 more
europepmc   +1 more source

Machine learning-guided optimization of atomic layer deposition process [PDF]

Minjong Lee, Doo San Kim, Thi Thu Huong Chu, Dushyant M. Narayan, Dan N. Le, Soubhik De, Soham Shirodkar, Seungbin Lee, Sihoon Son, Ji Eun Kang, Hyunbin Choi, Sun Jae Jeong, Taesung Kim, Geun Young Yeom, Si Joon Kim, Jiyoung Kim   +15 more
openalex   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source