Results 131 to 140 of about 38,860 (304)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Improvement of Phenol Degradation by UV Irradiation on Ultrasonic Atomized Droplets
Chemical Engineering TransactionsPhotocatalysis such as TiO2 particles are expected to be applied to the degradation of hazardous organic materials as an advanced oxidation processes (AOPs).
Daisuke Kobayashi, Hinano Watanabe
doaj
FEBS Open Bio, EarlyView.Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Liquid fuel spray processes in high-pressure gas flow [PDF]
Atomization of single liquid jets injected downstream in high pressure and high velocity airflow was investigated to determine the effect of airstream pressure on mean drop size as measured with a scanning radiometer.
Ingebo, R. D.
core +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Mathematical model for droplet size prediction of gas-water nozzle based on orthogonal experiment
Meikuang AnquanIn order to understand the influence of process parameters and structural parameters of internal mixing gas-water nozzle on atomization characteristics, based on a self-designed experimental platform for atomization characteristics, the influence of air ...
Xin WANG +5 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
AgriEngineeringThe use of unmanned aerial spraying systems is currently being explored and applied worldwide. The objective of this study was to characterize the droplet population generated by hydraulic nozzles and centrifugal atomization nozzles used in sprayers ...
Fábio Henrique Rojo Baio +5 more
doaj +1 more source
Big Data meets Quantum Chemistry Approximations: The $\Delta$-Machine Learning Approach
, 2015 Chemically accurate and comprehensive studies of the virtual space of all possible molecules are severely limited by the computational cost of quantum chemistry.
Dral, Pavlo O. +3 more
core