Results 111 to 120 of about 39,209 (300)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Fuel Processing TechnologySwirling atomization is an effective approach to improve atomization performance. The influence of swirling air on biodiesel spray and atomization characteristics was investigated via computational fluid dynamics simulations, focusing on the effects of ...
Shang Jiang +5 more
doaj +1 more source
Machine Learning, Quantum Mechanics, and Chemical Compound Space
, 2016 We review recent studies dealing with the generation of machine learning models of molecular and solid properties. The models are trained and validated using standard quantum chemistry results obtained for organic molecules and materials selected from ...
Adamo +106 more
core +1 more source
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Analysis of 5 KHz combustion instabilities in 40K methane/LOX combustion chambers [PDF]
In 40K methane/LOX 5 KHz engine tests, (first transverse mode) combustion instabilities observed by Rocketdyne are analyzed using Heidmann and Wieber's vaporization model to include LOX flow oscillations.
Breisacher, Kevin J., Priem, Richard J.
core +1 more source
Atomization of broad specification aircraft fuels [PDF]
, 1980 The atomization properties of liquid fuels for the potential use in aircraft gas turbine engines are discussed. The significance of these properties are addressed with respect to the ignition and subsequent combustion behavior of the fuel spray/air ...
Lefebvre, A. H., Skifstad, J. G.
core +1 more source
Norm-conserving pseudopotentials with chemical accuracy compared to all-electron calculations
, 2013 By adding a non-linear core correction to the well established Dual Space Gaussian type pseudopotentials for the chemical elements up to the third period, we construct improved pseudopotentials for the Perdew Burke Ernzerhof (PBE) functional and ...
Alex Willand +9 more
core +3 more sources
FEBS Open Bio, EarlyView.Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source