ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA
eLife, 2015 The cytosolic antiviral innate immune sensor RIG-I distinguishes 5′ tri- or diphosphate containing viral double-stranded (ds) RNA from self-RNA by an incompletely understood mechanism that involves ATP hydrolysis by RIG-I's RNA translocase domain ...
Charlotte Lässig +12 more
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The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase
Nature Communications, 2019 DnaB helicases are motor proteins that couple ATP-hydrolysis to the movement of the protein along single-stranded DNA leading to a separation of double-stranded DNA at the replication fork.
Thomas Wiegand +6 more
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Unraveling the Mystery of ATP Hydrolysis in Actin Filaments [PDF]
Journal of the American Chemical Society, 2014 Actin performs its myriad cellular functions by the growth and disassembly of its filamentous form. The hydrolysis of ATP in the actin filament has been shown to modulate properties of the filament, thus making it a pivotal regulator of the actin life ...
Martin McCullagh +2 more
semanticscholar +5 more sources
ATP hydrolysis and nucleotide exit enhance maltose translocation in the MalFGK2E importer [PDF]
Scientific Reports, 2021 ATP binding cassette (ABC) transporters employ ATP hydrolysis to harness substrate translocation across membranes. The Escherichia coli MalFGK2E maltose importer is an example of a type I ABC importer and a model system for this class of ABC transporters.
Bárbara Abreu +3 more
doaj +2 more sources
Beyond binding change: the molecular mechanism of ATP hydrolysis by F1-ATPase and its biochemical consequences [PDF]
Frontiers in Chemistry, 2023 F1-ATPase is a universal multisubunit enzyme and the smallest-known motor that, fueled by the process of ATP hydrolysis, rotates in 120o steps. A central question is how the elementary chemical steps occurring in the three catalytic sites are coupled to ...
Sunil Nath
doaj +2 more sources
Pathway of processive ATP hydrolysis by kinesin [PDF]
Nature, 1995 Direct measurement of the kinetics of kinesin dissociation from microtubules, the release of phosphate and ADP from kinesin, and rebinding of kinesin to the microtubule have defined the mechanism for the kinesin ATPase cycle. The processivity of ATP hydrolysis is ten molecules per site at low salt concentration but is reduced to one ATP per site at ...
Susan P. Gilbert +3 more
openalex +5 more sources
ATP-Binding and Hydrolysis in Inflammasome Activation [PDF]
Molecules, 2020 The prototypical model for NOD-like receptor (NLR) inflammasome assembly includes nucleotide-dependent activation of the NLR downstream of pathogen- or danger-associated molecular pattern (PAMP or DAMP) recognition, followed by nucleation of hetero ...
Christina F. Sandall +2 more
doaj +4 more sources
MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis [PDF]
Nature CommunicationsThe eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre ...
Sarah V. Faull +11 more
doaj +2 more sources
ATP-binding and hydrolysis of human NLRP3
Communications Biology, 2022 Analysis of the inflammasome-forming protein NLRP3 provides insights into the function of conserved residues in the ATP-binding site of NLRP3 and the correlation of ATP hydrolysis with inflammasome activation.
Rebecca Brinkschulte +10 more
doaj +3 more sources
Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation [PDF]
eLife, 2016 The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the
William John Allen +7 more
doaj +5 more sources