Results 291 to 300 of about 697,459 (324)

Soluble Apyrases Release ADP during ATP Hydrolysis

Biochemical and Biophysical Research Communications, 2001
A soluble form of CD39 was expressed and purified from High-Five insect cells. The soluble CD39 is a monomer with a molecular weight of 54,000. The k(cat) and K(m) of the purified soluble CD39 were 4.6 s(-1) and 12 microM for ATP and 1.3 s(-1) and 7 microM for ADP as substrates, respectively. One nucleotide binding site was detected on the monomer only
W, Chen, G, Guidotti
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On the Myosin Catalysis of ATP Hydrolysis

Biochemistry, 2004
Myosin is an ATP-hydrolyzing motor that is critical in muscle contraction. It is well established that in the hydrolysis that it catalyzes a water molecule attacks the gamma-phosphate of an ATP bound to its active site, but the details of these events have remained obscure.
Hirofumi, Onishi, Naoki, Mochizuki, Manuel F, Morales   +2 more
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Actin polymerization and ATP hydrolysis

Advances in Biophysics, 1990
This paper surveys several aspects of the consequences of ATP hydrolysis associated with actin polymerization, and their physiological implications. ATP hydrolysis occurs on F-actin in two subsequent reactions, cleavage of ATP followed by the slower release of Pi. The latter reaction is linked to a conformation change of the actin subunit that causes a
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ATP and GTP Hydrolysis Assays (TLC)

2013
Many biochemical reactions that occur within the cell are thermodynamically unfavorable. However, when these reactions are coupled to NTP (nucleoside triphosphate) hydrolysis, the energy derived from the hydrolysis of the phosphodiester bond helps drive the reaction in the favorable direction. Examples of such proteins can be found in almost all facets
Vaishnavi, Rajagopal, Jon R, Lorsch
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Coupling of kinesin steps to ATP hydrolysis

Nature, 1997
A key goal in the study of the function of ATP-driven motor enzymes is to quantify the movement produced from consumption of one ATP molecule. Discrete displacements of the processive motor kinesin along a microtubule have been reported as 5 and/or 8 nm.
W, Hua, E C, Young, M L, Fleming, J, Gelles   +3 more
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Mechanism of ATP hydrolysis dependent rotation of ATP synthases

2022
AbstractF1domain of ATP synthase is a rotary ATPase complex in which rotation of central γ-subunit proceeds in 120° steps against a surrounding α3β3fueled by ATP hydrolysis. How the ATP hydrolysis reactions occurring in three catalytic αβ dimers are coupled to mechanical rotation is a key outstanding question.
Atsuki Nakano, Jun-ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama   +3 more
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Stereochemical reaction mechanism formulations for enzyme-catalyzed pyrophosphate hydrolysis, ATP hydrolysis, and ATP synthesis

Bioorganic Chemistry, 1973
Abstract Fundamental concepts pertaining to the stereochemistry paths of polar additionelimination (nucleophilic substitution) reactions at phosphate phosphorus centers are reviewed and employed to analyze 18O exchange reactions catalyzed by inorganic pyrophosphatase and mitochondrial ATP synthetase.
Ephraim F. Korman, Jerome McLick
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ATP Hydrolysis and Synthesis Mechanisms

1997
A way to extend our knowledge of the mechanism of ATP synthesis catalyzed y the F1F0-complex is to elucidate the mechanism of its reverse reaction, the ATPase hydrolysis reaction. As we have indicted, ATP hydrolysis activity of the F1unit can be observed when the F1-F0-complex is decoupled from a protonmotive force or wen F1 is decoupled from F0.
Paul H. J. Nederkoorn, Henk Timmerman, Gabriëlle M. Donné-Op den Kelder   +2 more
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Mechanism of ATP hydrolysis by polymeric actin

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
The lag between polymerization of actin and ATP hydrolysis in actin filaments was analyzed in terms of the mechanism of the hydrolysis reaction. Under the experimental conditions (100 mM KCl and 1 mM MgCl2, or without KCl, 1 mM MgCl2 and 0.4 mM EGTA, 25 degrees C) ATP hydrolysis lagged behind polymerization by about 100 s independently of the ...
T, Ohm, A, Wegner
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Synthesis and hydrolysis of ATP by the mitochondrial ATP synthase

Biochemistry and Cell Biology, 1988
A brief summary of the factors that control synthesis and hydrolysis of ATP by the mitochondrial H+-ATP synthase is made. Particular emphasis is placed on the role of the natural ATPase inhibitor protein. It is clear from the existing data obtained with a number of agents that there is no correlation between variations of the rate of ATP hydrolysis ...
M T, Tuena Gómez-Puyou, O B, Martins, A, Gómez-Puyou   +2 more
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