Results 51 to 60 of about 697,459 (324)

Estimating the true stability of the prehydrolytic outward-facing state in an ABC protein

open access: yeseLife, 2023
CFTR, the anion channel mutated in cystic fibrosis patients, is a model ABC protein whose ATP-driven conformational cycle is observable at single-molecule level in patch-clamp recordings.
Márton A Simon   +3 more
doaj   +1 more source

The bacterial flagellar protein export apparatus processively transports flagellar proteins even with extremely infrequent ATP hydrolysis

open access: yesScientific Reports, 2014
For self-assembly of the bacterial flagellum, a specific protein export apparatus utilizes ATP and proton motive force (PMF) as the energy source to transport component proteins to the distal growing end.
T. Minamino   +4 more
semanticscholar   +1 more source

Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2.

open access: yesPLoS Biology, 2004
In response to activation by WASP-family proteins, the Arp2/3 complex nucleates new actin filaments from the sides of preexisting filaments. The Arp2/3-activating (VCA) region of WASP-family proteins binds both the Arp2/3 complex and an actin monomer and
Mark J Dayel, R Dyche Mullins
doaj   +1 more source

Influence of sulfite on ATPase activity of coupling factor CF(1) isolated from spinach chloroplasts [PDF]

open access: yesThe Ukrainian Biochemical Journal, 2019
The present study of sulfite participation in the regulation of the chloroplast ATP-synthase complex can help to understand the mechanisms of acid rains toxic effects on the plant photosynthetic apparatus.
O. B. Onoiko   +2 more
doaj   +1 more source

Unraveling the Mystery of ATP Hydrolysis in Actin Filaments

open access: yesJournal of the American Chemical Society, 2014
Actin performs its myriad cellular functions by the growth and disassembly of its filamentous form. The hydrolysis of ATP in the actin filament has been shown to modulate properties of the filament, thus making it a pivotal regulator of the actin life ...
Martin McCullagh   +2 more
semanticscholar   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Kinetic regularities and a possible mechanism of ATP non-enzymatic hydrolysis induced by calix[4]arene С-107 [PDF]

open access: yesThe Ukrainian Biochemical Journal
The kinetic model of calix[4]arene-induced ATP hydrolysis was elaborated. It is assumed that calix[4]­arene С-107 molecules form a complex with nucleoside triphosphate, ensuring the release of inorganic phosphate Рі, and then switch into an inactive ...
S. O. Kosterin   +5 more
doaj   +1 more source

Extracellular ATP Hydrolysis Inhibits Synaptic Transmission by Increasing pH Buffering in the Synaptic Cleft

open access: yesPLoS Biology, 2014
A slow mechanism of retinal synaptic inhibition involves hydrolysis of ATP released from pannexin 1 channels (from the tips of horizontal cell dendrites); the resulting protons and phosphates acidify the synaptic cleft, which inhibits neurotransmitter ...
Rozan Vroman   +6 more
semanticscholar   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

open access: yesFEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

open access: yesChem. Commun., 2014
Adenosine triphosphate is modified with two nitroxide radicals and employed to demonstrate that electron paramagnetic resonance spectroscopy can be used to study the enzymatic hydrolysis of doubly labelled substrate analogues.
Hacker, Stephan M.   +3 more
openaire   +4 more sources

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