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, 2023 Autoradiography, the direct imaging of radioactive distribution in tissue sections, is a powerful technique that has several key advantages for the validation of PET radiotracers. Using autoradiography, we can localize radiotracer uptake to neighbours of cells, and when multiplexed with additional radiotracers, fluorescent probes, or in situ tissue ...
Lewis, David Y.
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Autoradiography and Phosphorimaging
Cold Spring Harbor Protocols, 2020Many of the commonly used techniques in molecular cloning depend on methods to map accurately the distribution of radioactive atoms on two-dimensional (2D) surfaces. Without this ability, methods such as Southern blotting, northern hybridizations, radiolabeled DNA sequencing, and library screening would not have been possible.
Michael R, Green, Joseph, Sambrook
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Nonphotographic Alpha Autoradiography and Neutron-Induced Autoradiography
Science, 1970With a new combination of two techniques, (i) alpha-particle or fission fragment registration in thin polymer foils by etching and (ii) automatic counting and magnification of the etched perforations by local evaporation of a thin metal layer with an electric spark, the sensitivity of conventional photographic methods for determining quantity and ...
K, Becker, D R, Johnson
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Current Protocols in Protein Science, 1997
AbstractThis overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling.
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AbstractThis overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling.
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Current Protocols in Pharmacology, 1998
AbstractThe light‐microscopic localization of receptors and other molecules by autoradiography of radioligand binding in tissue sections provides a high degree of anatomical resolution and the ability to associate even small quantities of binding sites with anatomical structures. The basic procedure in this unit involves incubating slide‐mounted tissue
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AbstractThe light‐microscopic localization of receptors and other molecules by autoradiography of radioligand binding in tissue sections provides a high degree of anatomical resolution and the ability to associate even small quantities of binding sites with anatomical structures. The basic procedure in this unit involves incubating slide‐mounted tissue
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1963
In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer.
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In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer.
openaire +2 more sources