Results 21 to 30 of about 51,882 (244)
Bioluminometric Method for Real-Time Detection of Reverse Transcriptase Activity
BioTechniques, 1998 A simple and sensitive technique for detection of reverse transcriptase (RT) activity in real time has been developed. The technique is based on continuous detection of the inorganic pyrophosphate formed in the RT-catalyzed reaction by a luminometric ...
Samer Karamohamed +2 more
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BioTechniques, 1998 We have developed a simple and safe method for the determination of a transcription-initiation site. In this method, reverse transcriptase of the avian myeloblastosis virus or rTth DNA polymerase from Thermus thermopilus was used with a fluorescein ...
M. Yamada +4 more
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Virology Journal, 2011 For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method.
Zhang Zong-Ying +4 more
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A study on the isolation of leukemia virus of AKR mouse by fluorocarbon [PDF]
, 1962 The authors have succeeded in isolating a biologically-active leukemia virus from leukemic tissues of AKR mice with a fluorocarbon. From the chemical analysis of the biologically-active virus fraction it has been clarified that the AKR leukemia virus is ...
Miyoshi, Isao, Okada, Koichi
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BMC Genomics, 2020 Background Transcription factor (TF) GAMYB, belonging to MYB family (named after the gene of the avian myeloblastosis virus) is a master gibberellin (GA)-induced regulatory protein that is crucial for development and germination of cereal grain and ...
Anna Bienias +4 more
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BMC Microbiology, 2001 Background The hepadnaviral reverse transcriptase can synthesize DNA on its native RNA template within viral cores but it is usually unable to synthesize DNA employing exogenous nucleic acids as a template.
Tavis John E, Yao Ermei, Gong Yunhao
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Electron microscopy of Rous sarcoma virus genome RNA and its heat-dissociated subunits [PDF]
, 1975 Native and heat-treated RNAs from the purified Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fractionated by sucrose density gradients in the presence of ribonuclease inhibitor diethyl-pyrocarbonate and observed by electron microscopy.
Kanzaki, Yoshito
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Reverse Transcriptase Adds Nontemplated Nucleotides to cDNAs During 5′-RACE and Primer Extension
BioTechniques, 2001 In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5′-rapid amplification of cDNA ends (5′-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5′ termini that had not been reported before on any ...
Dayue Chen, John T. Patton
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In vitro transformation of lymphoid cells by Abelson murine leukemia virus [PDF]
, 1975 Cell cultures prepared from fetal murine liver were infected by Abelson murine leukemia virus. After about 2 weeks, proliferating cells of lymphoid morphology appeared in some of the cultures.
Baltimore, David +2 more
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BioTechniques, 1998 Fixed and paraffin-embedded tissues from pathology department archives are available for RNA expression analysis. We describe a general method for quantitation of specific RNA sequence extracted from single 6–8-μm human histological tissue sections cut ...
Giorgio Stanta, Serena Bonin
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