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Journal of Comparative Neurology, Volume 530, Issue 11, Page 1773-1949, August 2022., 2022 By using a genetically modified mouse model and viral tracing approaches, we mapped both the anterograde and the retrograde projections of a subpopulation of neurons in the anterior paraventricular thalamic nucleus, molecularly defined by the expression of glucokinase (GckaPVT).
Sevasti Gaspari +4 more
wiley +1 more source
Bioluminometric Method for Real-Time Detection of Reverse Transcriptase Activity
BioTechniques, 1998 A simple and sensitive technique for detection of reverse transcriptase (RT) activity in real time has been developed. The technique is based on continuous detection of the inorganic pyrophosphate formed in the RT-catalyzed reaction by a luminometric ...
Samer Karamohamed +2 more
doaj +1 more source
BioTechniques, 1998 We have developed a simple and safe method for the determination of a transcription-initiation site. In this method, reverse transcriptase of the avian myeloblastosis virus or rTth DNA polymerase from Thermus thermopilus was used with a fluorescein ...
M. Yamada +4 more
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Proceedings of the National Academy of Sciences of the United States of America, 1973 Two structurally distinct forms of RNA-directed DNA polymerase from avian myeloblastosis virus were resolved by chromatography on phosphocellulose and purified. In addition to RNA-directed DNA polymerase activity, both enzymes had ribonuclease H (RNase H)
D. Grandgenett, G. Gerard, M. Green
semanticscholar +1 more source
Virology Journal, 2011 For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method.
Zhang Zong-Ying +4 more
doaj +1 more source
BMC Genomics, 2020 Background Transcription factor (TF) GAMYB, belonging to MYB family (named after the gene of the avian myeloblastosis virus) is a master gibberellin (GA)-induced regulatory protein that is crucial for development and germination of cereal grain and ...
Anna Bienias +4 more
doaj +1 more source
BMC Microbiology, 2001 Background The hepadnaviral reverse transcriptase can synthesize DNA on its native RNA template within viral cores but it is usually unable to synthesize DNA employing exogenous nucleic acids as a template.
Tavis John E, Yao Ermei, Gong Yunhao
doaj +1 more source
Mechanism of action of ribonuclease H isolated from avian myeloblastosis virus and Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America, 1973 Purified preparations of RNA-dependent DNA polymerase isolated from avain myeloblastosis virus contain RNase H activity. Labeled ribohomopolymers are degraded in the presence of their complementary deoxyribopolymer, except [(3)H]poly(U).poly(dA).
J. Leis, I. Berkower, J. Hurwitz
semanticscholar +1 more source
Reverse Transcriptase Adds Nontemplated Nucleotides to cDNAs During 5′-RACE and Primer Extension
BioTechniques, 2001 In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5′-rapid amplification of cDNA ends (5′-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5′ termini that had not been reported before on any ...
Dayue Chen, John T. Patton
doaj +1 more source
Avian myeloblastosis virus proteins in leukemic chicken myeloblasts [PDF]
Journal of Virology, 1980 We have analyzed the avian myeloblastosis virus proteins in two types of leukemic myeloblasts: established myeloblastic cell lines (DU 1765 and DU 11157) and leukemic myeloblasts obtained from the peripheral blood of a leukemic C/E Spafas chicken (no. 21957). Using monospecific antisera for immunoprecipitation and polyacrylamide gel electrophoresis, we
M A Baluda, R F Silva
openaire +3 more sources