Results 81 to 90 of about 906,003 (303)

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Performance-Based Multi-Objective Optimization of Four-Limb CFST Lattice Columns

Buildings
In this paper, the low-cycle reciprocating load test was carried out on four-limb concrete-filled steel tubular (CFST) lattice columns with different slenderness ratios and axial compression ratios, and the seismic performance was studied.
Junjie He, Zhi Huang, Juan Chen, Wangbao Zhou, Tao Huang, Xin Kang, Yohchia Frank Chen   +6 more
doaj   +1 more source

Design and Experimental Validation of a Ducted Counter-rotating Axial-flow Fans System [PDF]

, 2012
An experimental study on the design of counter-rotating axial-flow fans was carried out. The fans were designed using an inverse method. In particular, the system is designed to have a pure axial discharge flow.
NOURI, Hussain, BAKIR, Farid, RAVELET, Florent, REY, Robert, F. Bakir, R. Rey, H. Nouri, F. Ravelet, SARRAF, Christophe, C. Sarraf   +9 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Experimental Study on and Finite Element Analysis of the Axial Compression Bearing Capacity of a UHPC Transfer Device for Pre-Stressed Anti-Floating Anchor Rods

Buildings
A new UHPC pre-stress transfer device is proposed for pre-stressed anti-floating anchor rods. To investigate the axial compression performance of the device during pre-stressing, physical experiments and a finite element verification were conducted on ...
Yi Zhu, Yi Li, Zhen-Xing Tang, Pei-Lin Liu, Zhuo Yang   +4 more
doaj   +1 more source

Electromagnetic Force Distribution and Axial Deformation Uniformity Analysis of Dual-Coil Electromagnetic Tube Compression Method

IEEE Access, 2020
In the conventional electromagnetic tube expansion, axial distribution of the electromagnetic force is not uniform along the workpiece. This results in uneven axial deformation of the deformed tube and restricts the full industrial implementation of this
Li Qiu, Yudong Wang, Zhang Wang, Ahmed Abu-Siada, Yantao Li, Lan Jiang   +5 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Local stability analysis of axial compression members with welded box-shaped section of Q690 high strength steel

上海师范大学学报. 自然科学版
High strength steel welded box-shaped axial compression members have been widely used in steel structure engineering. In this paper, ANSYS analysis software was adopted to establish a finite element model considering the local geometrical initial defect ...
ZHAO Chuandou, YANG Yonghua
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source