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Characterization of Azospirillum brasilense Lipopolysaccharides

Mikrobiolohichnyi Zhurnal, 2022
Azospirillum brasilense is a gram-negative, nitrogen-fixing bacterium that colonizes the rhizosphere of various types of grasses and cereals. Lipopolysaccharides (LPS) are a class of complex glycolipids present in the cell membrane of gramnegative bacteria and mediate plant-bacteria interactions. Although the effects of LPS of pathogenic plant bacteria
T.V. Bulyhina, L.D. Varbanest
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Biosynthesis of gold nanoparticles by Azospirillum brasilense

Microbiology, 2013
Plant-associated nitrogen-fixing soil bacteria Azospirillum brasilense were shown to reduce the gold of chloroauric acid to elemental gold, resulting in formation of gold nanoparicles. Extracellular phenoloxidizing enzymes (laccases and Mn peroxidases) were shown to participate in reduction of Au+3 (HAuCl4) to Au(0).
M A, Kupriashina   +4 more
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Mutation in Azospirillum Brasilense

1985
In order to study mutation frequencies in A. brasilense two selective systems, i. e. resistance to 5-Fluorotryptophan and to Rifampicin, were set up. The rate of mutation in this organism after treatments with several mutagens has been investigated.
M. Maddalena Del Gallo   +2 more
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Polyhydroxybutyrate in Azospirillum brasilense

2015
Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate produced by several bacteria as carbon storage and reductive equivalents sink. The production of PHB has been reported for several plant-associated bacteria, and especially in Azospirillum brasilense, the production of PHB has been associated as a factor for stress endurance.
Marcelo Müller-Santos   +3 more
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Construction of an Azospirillum brasilense Sp7 recA mutant

Molecular and General Genetics MGG, 1990
Cosmid clones encoding the recA gene of Azospirillum brasilense were isolated by intergeneric complementation of an Escherichia coli recA mutant. Site-directed Tn5 mutagenesis and subcloning of one complementing cosmid clone allowed us to localize the A. brasilense recA gene on a 1.2 kb DNA fragment.
C, Croes, J, Vanderleyden, K, Michiels
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Identification of the glutamine synthetase adenylyltransferase of Azospirillum brasilense

Research in Microbiology, 2009
Glutamine synthetase, a key enzyme in nitrogen metabolism of both prokaryotes and eukaryotes, is strictly regulated. One means of regulation is the modulation of activity through adenylylation catalyzed by adenylyltransferases. Using PCR primers based on conserved sequences in glutamine synthetase adenylyltransferases, we amplified part of the glnE ...
Anne, Van Dommelen   +2 more
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Identification of a nifW-like gene in Azospirillum brasilense

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1993
A small ORF, 5' upstream of the fixABC operon in Azospirillum brasilense Sp7, has been identified. Sequence comparison shows significant homology to the Azotobacter vinelandii and Azorhizobium caulinodans nifW gene.
A, Milcamps, V, Keyers, J, Vanderleyden
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Mechanistic studies on Azospirillum brasilense glutamate synthase

Biochemistry, 1991
The reaction mechanism of Azospirillum brasilense glutamate synthase has been investigated by several approaches. 15N nuclear magnetic resonance studies demonstrate that the amide nitrogen of glutamine is reductively transferred to 2-oxoglutarate in an irreversible manner with no release of the transferred ammonia group into the medium.
M A, Vanoni   +4 more
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Effects of coinoculation of Azospirillum brasilense and Bradyrhizobium spp. in soybean and inoculation of Azospirillum brasilense in corn

Caderno Pedagógico
Soybean and corn are widely cultivated crops in Brazil and worldwide, and are raw materials for several industrial products. This work aimed to evaluate the co-inoculation of Azopirillum brasilense and Bradyrhizobium japonicum in soybean crops, as well as the inoculation of A. brasilense in corn in diferent locations of Goiás.
Elizabete Lourenço Pires   +5 more
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