Results 61 to 70 of about 4,818 (173)
Transformation of Azotobacter vinelandii with plasmid DNA [PDF]
Azotobacter vinelandii cells can be transformed at high frequencies with the broad-host-range plasmids pRK2501, RSF1010, and pGSS15, using a modification of the procedure developed by Page and von Tigerstrom (J. Bacteriol. 139:1058-1061, 1979) for chromosomal DNA-mediated transformation.
B R, Glick, H E, Brooks, J J, Pasternak
openaire +2 more sources
Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers
Microbial biosynthesis and engineered enzyme platforms are expanding the design space of polyhydroxyalkanoate bioplastics. By combining fermentation, PHA synthase engineering and cell‐free modular systems, tailored biodegradable polymers can be produced with tunable properties, supporting more sustainable materials and future circular bioeconomy ...
Giovanni Gallo +4 more
wiley +1 more source
Identification of sulfurtransferase enzymes in Azotobacter vinelandii [PDF]
Rhodanese and 3‐mercaptopyruvate sulphurtransferase have been identified in A. vinelandii. Two distinct active fractions of the two sulphurtransferases were obtained after FPLC ion‐exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of M r ca 25
Pagani, S. +3 more
openaire +2 more sources
Azotobacter vinelandii, a strict aerobic, nitrogen fixing bacterium in the Pseudomonadaceae family, exhibits a preferential use of acetate over glucose as a carbon source.
Elva Quiroz-Rocha +8 more
doaj +1 more source
This review maps how electroenzymatic cascades that couple nitrogen‐cycle enzymes with C─N bond forming auxilary enzymes, powered by direct electron transfer (DET) or mediated electron transfer (MET), can upgrade N2 and waste nitrogen streams into value‐added amines, amides, amino acids, and other nitrogen‐based chemicals.
Rohit G. Jadhav +4 more
wiley +1 more source
Effect of bio-fertilizers and nitrogen on quantitative and qualitative characteristics of tepals in saffron (Crocus sativus L.) [PDF]
In order to investigate the effect of different nitrogen sources on some quantitative and biochemical characteristics of Saffron Petals, an experiment was conducted based on randomized complete block design with three replications on the Research Farm of
Azizollah Kheiry +3 more
doaj +1 more source
Biotic–Abiotic Hybrid Strategies for Photosynthetic Nitrogen Fixation and Green Ammonia Synthesis
Biotic‐abiotic photosynthetic systems integrate light‐harvesting semiconductors with nitrogenase's selective N2 reduction under ambient conditions, offering a sustainable alternative to conventional ammonia synthesis. Advancing rational interface design, efficient energy‐reaction coupling, and system integration is critical to realizing scalable, solar‐
Minghua Xu +3 more
wiley +1 more source
A CRISPR interference system for engineering biological nitrogen fixation
A grand challenge for the next century is in facing a changing climate through bioengineering solutions. Biological nitrogen fixation, the globally consequential, nitrogenase-catalyzed reduction of atmospheric nitrogen to bioavailable ammonia, is a vital
Steven J. Russell +2 more
doaj +1 more source
Microbial exopolysaccharides (EPS) enhance plant stress tolerance and soil health by improving water retention, nutrient cycling, and resilience to salinity and heavy metals, offering a biodegradable and eco‐friendly alternative to synthetic polymers for sustainable agriculture. ABSTRACT Microbial exopolysaccharides (EPSs) serve multiple industrial and
Aishmita Gantait +4 more
wiley +1 more source
A polyethylene surrogate sample containing paraffin, polywax and polyethylene was exposed to selected microbes identified in this study. This image shows the surface of this surrogate following a six‐week incubation with Rhodococcus jostii. ABSTRACT Polyethylene represents a particularly recalcitrant class of plastics that persist for decades in the ...
Bilge Bahar Camur +5 more
wiley +1 more source

