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Immunological responses and protective efficacy against Brucella melitensis induced by bp26 and omp31 B. melitensis Rev.1 deletion mutants in sheep

Vaccine, 2007
The commonly used live attenuated vaccine in ovine brucellosis prophylaxis is Brucella melitensis Rev.1. This vaccine is known to induce antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in challenged animals.
Jacques, Isabelle   +8 more
openaire   +4 more sources

OtpR regulated the growth, cell morphology of B. melitensis and tolerance to β-lactam agents

Veterinary Microbiology, 2012
The intracellular pathogen, Brucella melitensis, possesses an operon with two components: otpR (BMEI0066), which encodes a response regulator, and BMEI0067, which encodes a putative cAMP-dependent protein kinase regulatory subunit. Previous studies have shown that a polar mutation in the BMEI0066 gene significantly decreased virulence and stress ...
Wenjuan, Liu   +5 more
openaire   +3 more sources

An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination

Veterinary Microbiology, 1992
An indirect enzyme-linked immunosorbent assay (ELISA) with unpurified Brucella melitensis smooth lipopolysaccharide (S-LPS) as antigen was evaluated for the serological diagnosis of B. melitensis infection in sheep in comparison with the Rose Bengal (RB), complement fixation (CF), radial immunodiffusion (RID), microplate agglutination (MA) and rivanol ...
M P, Jiménez de Bagüés   +4 more
openaire   +3 more sources

The lipopolysaccharides of Brucella abortus and B. Melitensis

Annales de l'Institut Pasteur / Microbiologie, 1987
Bundle, D.   +3 more
semanticscholar   +5 more sources

Fluorescence signal amplification assay for the detection of B. melitensis 16M, based on peptide-mediated magnetic separation technology and a AuNP-mediated bio-barcode assembled by quantum dot technology.

In Analysis, 2019
Members of the Brucella spp. are facultative intracellular bacteria that can cause global brucellosis, a zoonotic disease. Herein, a novel fluorescence signal amplification (FSA) method for the rapid detection of B.
Xinxin Li   +11 more
semanticscholar   +1 more source

Genome-Wide Analysis of the Response of Brucella melitensis NI to Polymyxin B

Current Pharmaceutical Biotechnology, 2021
Background: The ability of pathogenic bacteria to survive Antimicrobial Peptides (AMPs) in various host niches may contribute to their virulence. Polymyxin B is a cationic AMP, and polymyxin drugs are considered to be the "last line of defense" in the clinical treatment of bacterial infections.
Zhen Wang   +5 more
openaire   +2 more sources

Identification of Brucella abortus, B. canis, B. melitensis, and B. suis by carbon substrate assimilation tests

Veterinary Microbiology, 2001
By using the results of seven carbon substrate assimilation tests from the Biotype 100 system (bioMérieux, Marcy-l'Etoile, France), we correctly identified 79 (85.9%) of 92 Brucella strains tested. The specificity of the method varied from 97.4 to 100% depending on the species.
A, López-Merino   +6 more
openaire   +2 more sources

Rapid identification of human B-lymphocytes and monocytes with rhodamine-labeled Brucella melitensis

Journal of Immunological Methods, 1981
The bacterium Brucella melitensis has been shown to bind selectively to human B-lymphocytes. The specificity of this binding can be exploited in a rapid technique for the determination of human B-lymphocytes and monocytes by use of rhodamine-labeled Brucella melitensis.
G, Gaudernack, R, Curry, R, Messner
openaire   +2 more sources

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