Results 161 to 170 of about 12,366,515 (198)

An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination

Veterinary Microbiology, 1992
An indirect enzyme-linked immunosorbent assay (ELISA) with unpurified Brucella melitensis smooth lipopolysaccharide (S-LPS) as antigen was evaluated for the serological diagnosis of B. melitensis infection in sheep in comparison with the Rose Bengal (RB), complement fixation (CF), radial immunodiffusion (RID), microplate agglutination (MA) and rivanol ...
M P, Jiménez de Bagüés, C M, Marín, J M, Blasco, I, Moriyón, C, Gamazo   +4 more
openaire   +2 more sources

Rapid identification of human B-lymphocytes and monocytes with rhodamine-labeled Brucella melitensis

Journal of Immunological Methods, 1981
The bacterium Brucella melitensis has been shown to bind selectively to human B-lymphocytes. The specificity of this binding can be exploited in a rapid technique for the determination of human B-lymphocytes and monocytes by use of rhodamine-labeled Brucella melitensis.
G, Gaudernack, R, Curry, R, Messner
openaire   +2 more sources

Overproduction of the Brucella melitensis heat shock protein DnaK in Escherichia coli and its localization by use of specific monoclonal antibodies in B. melitensis cells and fractions

Research in Microbiology, 1996
The Brucella melitensis dnaK gene was amplified by the polymerase chain reaction using primers chosen according to the published sequence of B. ovis and cloned in multiple copy plasmids enabling expression under the control of the Plac promoter. Monoclonal antibodies (mAb) obtained by immunizing mice with B.
Cloeckaert, A, Grépinet, O, Debbarh, H.Salih-Alj, Zygmunt, M.S   +3 more
openaire   +4 more sources

B. melitensis rough strain B115 is protective against heterologous Brucella spp. infections

Vaccine, 2011
Brucellosis is one of the most serious zoonoses all over the world, with B. melitensis, B. abortus and B. suis being the most pathogenic species for humans. Vaccination of domesticated livestock still represents the most efficient way to prevent human infection.
Rosanna, Adone, Massimiliano, Francia, Claudia, Pistoia, Michele, Pesciaroli, Paolo, Pasquali   +4 more
openaire   +2 more sources

Type III Secretion Homologs Are Present in Brucella melitensis , B. ovis , and B. suis biovars 1, 2, and 3

Current Microbiology, 2003
Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and ...
Abdallah, AI, Commander, NJ, Woodward, MJ, Spencer, S, Hart, CA, Winstanley, C   +5 more
openaire   +4 more sources

OtpR regulated the growth, cell morphology of B. melitensis and tolerance to β-lactam agents

Veterinary Microbiology, 2012
The intracellular pathogen, Brucella melitensis, possesses an operon with two components: otpR (BMEI0066), which encodes a response regulator, and BMEI0067, which encodes a putative cAMP-dependent protein kinase regulatory subunit. Previous studies have shown that a polar mutation in the BMEI0066 gene significantly decreased virulence and stress ...
Wenjuan, Liu, Hao, Dong, Wenxiao, Liu, Xiaolei, Gao, Chunyan, Zhang, Qingmin, Wu   +5 more
openaire   +2 more sources

A review of the use of B. melitensis Rev 1 vaccine in adult sheep and goats

Preventive Veterinary Medicine, 1997
The live Brucella melitensis Rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. The classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world.
openaire   +2 more sources

The lipopolysaccharides of Brucella abortus and B. Melitensis

Annales de l'Institut Pasteur / Microbiologie, 1987
Bundle, D., Cherwonogrodzky, J., Caroff, M., Perry, Malcolm   +3 more
openaire   +4 more sources

Comparison of B. melitensis Rev. 1 and B. abortus strain 19 as a Vaccine against Brucellosis in Cattle

Zentralblatt für Veterinärmedizin Reihe B, 2010
Summary The living attenuated Brucella melitensis Rev. 1 vaccine was compared with the B. abortus strain 19 vaccine, for its suitability in protecting pregnant cows against challenge with virulent Brucella. Twenty calves were vaccinated with each vaccine and 10 animals were left unvaccinated as controls.
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Performance of skin tests with allergens from B. melitensis B115 and rough B. abortus mutants for diagnosing swine brucellosis

Veterinary Microbiology, 2014
Swine brucellosis by Brucella suis biovar 2 is an emerging disease whose control is based on serological testing and culling. However, current serological tests detect antibodies to the O-polysaccharide (O/PS) moiety of Brucella smooth lipopolysaccharide (S-LPS), and thus lack specificity when infections by Yersinia enterocolitica O:9 and other gram ...
L, Dieste-Pérez, J M, Blasco, M J, De Miguel, C M, Marín, M, Barberán, R, Conde-Álvarez, I, Moriyón, P M, Muñoz   +7 more
openaire   +2 more sources