Results 101 to 110 of about 842 (140)

Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis

Veterinary Parasitology, 1997
The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted
A, Brüning, P, Phipps, E, Posnett, E U, Canning   +3 more
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The interaction of Babesia caballi kinetes with tick cells

Journal of Invertebrate Pathology, 1983
Abstract Babesia caballi caused pathological changes in the vector tick, Anocentor nitents. These included the loss of hemocytes, reddish-brown discoloration of the hemolymph, retardation in clotting, and reduction in egg production. Primary cultures were initiated from A. nitens embryos transovarially infected with B. caballi.
T J, Kurtti, U G, Munderloh, D, Stiller
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Babesia caballi and Babesia equi: Implications of host sialic acids in erythrocyte infection

Experimental Parasitology, 2005
The present study investigated the involvement of host sialic acids in the erythrocyte infection by two equine Babesia parasites, Babesia equi and Babesia caballi. We observed that the in vitro growth of both parasites is influenced by the removal of sialic acids from the surface of equine erythrocytes (RBC).
Masashi, Okamura, Naoaki, Yokoyama, Nilanthi P A L K, Wickramathilaka, Noriyuki, Takabatake, Yuzuru, Ikehara, Ikuo, Igarashi   +5 more
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Analysis of a Growth-Promoting Factor forBabesia caballiCultivation

Journal of Parasitology, 2001
Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21.
H, Ikadai, M D, Martin, H, Nagasawa, K, Fujisaki, N, Suzuki, T, Mikami, N, Kudo, T, Oyamada, I, Igarashi   +8 more
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Polyclonal antibody characterization of Babesia caballi antigens

International Journal for Parasitology, 1994
In a previous study diagnostic B. caballi antigens with apparent molecular mass of 50 and 48 kDa were identified. Another antigen of 141 kDa was recognized by most but not all B. caballi sera tested. Here a further characterization of the three antigens is reported.
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Ultrastructural characteristics of Babesia caballi in equine erythrocytes in vitro

Parasitology Research, 1999
Babesia caballi cultured continuously in equine erythrocytes was examined by transmission electron microscopy. The use of cultured B. caballi permitted examination of a large number of parasitized cells with various stages of intra erythrocytic development. The piriform merozoites of B.
S, Kawai, I, Igarashi, A, Abgaandorjiin, K, Miyazawa, H, Ikadai, H, Nagasawa, K, Fujisaki, T, Mikami, N, Suzuki, H, Matsuda   +9 more
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Continuous in vitro cultivation of Babesia caballi in serum-free medium

Parasitology Research, 1999
Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated
E, Zweygarth, C J, van Niekerk, D T, de Waal   +2 more
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Culture, isolation and propagation of Babesia caballi from naturally infected horses

Parasitology Research, 2002
Thirteen blood samples of horses from South Africa, five of which were seropositive for Babesia caballi and eight for both B. caballi and Theileria equi, were subjected to in vitro culture to identify carrier animals. None of the animals had a detectable parasitaemia on Giemsa-stained blood smears before culture initiation. Cultures were initiated in L-
Erich, Zweygarth, Laura M, Lopez-Rebollar, Jane, Nurton, Alan J, Guthrie   +3 more
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INHIBITORY EFFECT OF LACTOFERRIN ON IN VITRO GROWTH OF BABESIA CABALLI

The American Journal of Tropical Medicine and Hygiene, 2005
Lactoferrin (LF) is an important biologic molecule with many functions, one of which is antimicrobial defense. We evaluated the growth-inhibiting effects of four types of LF (native LF, Fe+3-bound [holo] LF, Fe+3-free [apo] LF, and LF hydrolyzate) on the in vitro growth of Babesia caballi and B. equi. The growth of B.
Hiromi, Ikadai, Tetsuya, Tanaka, Nona, Shibahara, Hiroko, Tanaka, Aya, Matsuu, Noboru, Kudo, Kei-ichi, Shimazaki, Ikuo, Igarashi, Takashi, Oyamada   +8 more
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Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi

International Journal for Parasitology, 1999
Monoclonal antibodies (mAbs) were produced against Babesia caballi (USDA strain) to define a species-specific antigen for use in diagnosis and vaccine development. Eight positive clones of B. caballi mAbs determined by indirect immunofluorescent antibody test were selected for purification and further characterisation.
H, Ikadai, Y, Tamaki, X, Xuan, I, Igarashi, S, Kawai, H, Nagasawa, K, Fujisaki, Y, Toyoda, N, Suzuki, T, Mikami   +9 more
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