Babesia caballi and Babesia equi: Implications of host sialic acids in erythrocyte infection
Experimental Parasitology, 2005The present study investigated the involvement of host sialic acids in the erythrocyte infection by two equine Babesia parasites, Babesia equi and Babesia caballi. We observed that the in vitro growth of both parasites is influenced by the removal of sialic acids from the surface of equine erythrocytes (RBC).
Masashi, Okamura +5 more
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INHIBITORY EFFECT OF LACTOFERRIN ON IN VITRO GROWTH OF BABESIA CABALLI
The American Journal of Tropical Medicine and Hygiene, 2005Lactoferrin (LF) is an important biologic molecule with many functions, one of which is antimicrobial defense. We evaluated the growth-inhibiting effects of four types of LF (native LF, Fe+3-bound [holo] LF, Fe+3-free [apo] LF, and LF hydrolyzate) on the in vitro growth of Babesia caballi and B. equi. The growth of B.
Hiromi, Ikadai +8 more
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Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi
International Journal for Parasitology, 1999Monoclonal antibodies (mAbs) were produced against Babesia caballi (USDA strain) to define a species-specific antigen for use in diagnosis and vaccine development. Eight positive clones of B. caballi mAbs determined by indirect immunofluorescent antibody test were selected for purification and further characterisation.
H, Ikadai +9 more
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Parasitological and molecular diagnostic of a clinical Babesia caballi outbreak in Southern Romania [PDF]
Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describe a clinical B. caballi outbreak in
Ioan Liviu Mitrea +2 more
exaly +3 more sources
Ultrastructural characteristics of Babesia caballi in equine erythrocytes in vitro
Parasitology Research, 1999Babesia caballi cultured continuously in equine erythrocytes was examined by transmission electron microscopy. The use of cultured B. caballi permitted examination of a large number of parasitized cells with various stages of intra erythrocytic development. The piriform merozoites of B.
S, Kawai +9 more
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Analysis of a Growth-Promoting Factor forBabesia caballiCultivation
Journal of Parasitology, 2001Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21.
H, Ikadai +8 more
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Polyclonal antibody characterization of Babesia caballi antigens
International Journal for Parasitology, 1994In a previous study diagnostic B. caballi antigens with apparent molecular mass of 50 and 48 kDa were identified. Another antigen of 141 kDa was recognized by most but not all B. caballi sera tested. Here a further characterization of the three antigens is reported.
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Continuous in vitro cultivation of Babesia caballi in serum-free medium
Parasitology Research, 1999Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated
E, Zweygarth +2 more
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Culture confirmation of the carrier status of Babesia caballi-infected horses
Journal of Clinical Microbiology, 1993Culture of horse blood for Babesia caballi identified four carrier horses among nine previously infected horses. Three of the carriers had no detectable parasitemias on stained blood smears, and sera from two carrier horses were complement fixation test negative. Three cultures were continuously cultivated. Cryopreserved fourth-passage B.
P J, Holman +3 more
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Seroprevalence of Babesia caballi and Theileria equi in the Swiss horse population
Parasitology International, 2010In Switzerland, the prevalence and incidence of equine piroplasma parasite (EPP) infections are unknown. In order to obtain a first insight into the prevalence, a representative sample of 689 sera of horses from Switzerland was serologically tested for the presence of antibodies directed against T. equi and B.
Sigg L +4 more
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