Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis [PDF]
Archives of Razi Institute, 2021 Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections.
L Rahbarnia +3 more
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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples [PDF]
Revista Brasileira de Parasitologia Veterinária, 2022 The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds.
Bruna Nicoleti Santana +4 more
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Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets. [PDF]
PLoS ONE, 2015 Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences.
Guoqin Yu +4 more
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Detection of Plasmodium vivax by Nested PCR and Real-Time PCR [PDF]
The Korean Journal of Parasitology, 2010 Malaria is endemic in the Cukurova region while it is sporadic in other regions of Turkey. Therefore, the laboratory and clinical diagnosis of malaria is important for the treatment of malaria. In this study, 92 blood samples that were taken from the suspected malaria patients for routine diagnosis in a period of 10 years between 1999 and 2009 were ...
Genc A., Eroglu F., Koltas I.S.
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Nested PCR for Diagnosis of Typhoid Fever [PDF]
Journal of Clinical Microbiology, 2005 The superiority of the nested PCR employing H 1-d primers over culture or single-dilution Widal (4) should be reevaluated for isolates from unusual sites and from among those coinfected with human immunodeficiency virus (HIV)/AIDS. Numerous extraintestinal complications are known with Salmonella enterica serovar Typhi infection.
Subhash C, Arya +4 more
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Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido [PDF]
, 2010 INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Dionízio Filho, Pedro Sebastião Raimundo +5 more
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APPLICATION OF NESTED REVERSE TRANSCRIPTION PCR FOR DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN SEMEN [PDF]
Veterinary Medical Journal - Giza, 2003 A nested reverse transcription-polymerase chain reaction (RT-PCR) was employed for detection of bovine viral diarrhea virus (BVDV) in 59 semen samples collected from farms of known history of BVDV infection. The results indicated that 6 (10.2%) and 8 (13.
A AMIN +2 more
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Identification of potential insect vectors of the Cape Saint Paul Wilt Disease of coconut in Ghana by PCR [PDF]
, 2009 The vector of the phytoplasma responsible for the coconut lethal yellowing disease in West Africa is unknown to date. However, it is known that phytoplasmas are transmitted by leafhoppers and planthoppers, which are supposed to be the only ones able to ...
Descamps, Sophie +7 more
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Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene [PDF]
, 2016 Background: Nested PCRs based on the Plasmodium 18s-rRNA gene have been extensively used for human malaria diagnosis. However, they are not practical when large quantities of samples need to be processed, further there have been challenges in the ...
Diego F. Echeverry +10 more
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Research in Plant Disease, 2015 The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim +4 more
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