Results 1 to 10 of about 100,489 (216)

Performance of nested-RT-PCR assays for Zika and Dengue detection

Acta Scientiarum: Biological Sciences
Arboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses.
José Henrique Francisco Roma, Rachel Cruz Alves, Stefhano Luis Candido, Valéria Dutra, Luciano Nakazato, Renata Dezengrini-Slhessarenko, Juliana Helena Chavez-Pavoni, Mariângela Ribeiro Resende   +7 more
doaj   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

Malaria Journal, 2012
Background A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes.
Lee Pei-Wen, Ji Dar-Der, Liu Chia-Tai, Rampao Herodes S, do Rosario Virgilio E, Lin I-Feng, Shaio Men-Fang   +6 more
doaj   +1 more source

Nested RT-PCR for ante mortem diagnosis of rabies from body secretion/excretion of animals suspected for rabies

Veterinary World, 2012
Aim: The present study deals with molecular technique Nested RT-PCR for detection of rabies viral RNA from biological fluid samples (Saliva, Milk and Urine) collected from animal suspected for rabies and to compare the sensitivity of Nested RT-PCR ...
M Dandale, C K Singh, V Ramneek, D Deka, B S Sandhu, K Bansal, N K Sood   +6 more
doaj   +1 more source

Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge

Archives of Environmental Protection, 2014
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra, Wiszniowski Jarosław, Ciesielski Sławomir   +2 more
doaj   +1 more source

Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical, 2007
A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR) foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do ...
Karla Valéria Batista Lima, Maria Luíza Lopes, Edvaldo Carlos Brito Loureiro, Maurimélia Mesquita Costa, Ninarosa Calzavara Cardoso, George Leandro Ferreira Lima, Maísa Silva Sousa   +6 more
doaj   +1 more source

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquímica e nested-PCR

Revista Brasileira de Parasitologia Veterinária, 2009
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB).
Aline Diniz Cabral, Clarice Neves Camargo, Nara Thiers Cacciatori Galleti, Liria Hiromi Okuda, Edviges Maristela Pituco, Claudia Del Fava   +5 more
doaj   +1 more source

Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Universa Medicina, 2007
Latar Belakang DNA rpob (RNA polymerase sub unit b) Mycobacterium tuberculosis dapat diamplifikasi secara spesifik dengan metode nested polymerase chain reaction (PCR).
Maria Lina Rosilawati
doaj   +1 more source

Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome

Memorias do Instituto Oswaldo Cruz, 2004
We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS ...
Marcos Lázaro Moreli, Ricardo Luiz Moro de Sousa, Luiz Tadeu Moraes Figueiredo   +2 more
doaj   +1 more source

Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology, 2006
world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several ...
Nastiti Wijayanti, Hera Nirwati, Tri Wibawa, Aris Haryanto, S. Sutaryo   +4 more
doaj   +1 more source