Results 41 to 50 of about 188,318 (309)

Molecular Genotyping of HBV by using Nested PCR-RFLP among Hepatitis B Patients in Daerah Istimewa Yogyakarta Province and Surrounding Area

Indonesian Journal of Biotechnology, 2008
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto, Nenny Sri Mulyani, Titis Widowati, Nastiti Wijayanti, Purnomo Hadi   +4 more
doaj   +1 more source

White spot syndrome virus (WSSV) transmission risk through infected cooked shrimp products assessed by polymerase chain reaction (PCR) and bio-inoculation studies [PDF]

, 2011
The aim of the study was to evaluate the resistance of white spot syndrome virus (WSSV) in shrimps (Penaeus monodon) to the process of cooking. The cooking was carried out at 1000C six different durations 5, 10, 15, 20, 25 and 30 min.
Devivaraprasad Reddy, A.A., Jeya Shakila, R., Jeyasekaran, G.   +2 more
core  

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Reliability of Rapid Diagnostic Tests in Diagnosing Pregnancy-Associated Malaria in North-Eastern Tanzania. [PDF]

, 2012
Accurate diagnosis and prompt treatment of pregnancy-associated malaria (PAM) are key aspects in averting adverse pregnancy outcomes. Microscopy is the gold standard in malaria diagnosis, but it has limited detection and availability.
A Mayor, A Moody, A Muehlenbachs, A Salanti, AA Adegnika, Adrian JF Luty, AE Mnzava, AHD Kilian, AJ Umbers, Ali Salanti, AM de Oliveira, B Mmbando, C Wongsrichanalai, Caroline Pehrson, CE Shulman, CE Shulman, Christentze Schmiegelow, D Ishengoma, Daniel Andersen, Daniel TR Minja, Davis John, DP Mason, DR Bell, DRS Ishengoma, E Tjitra, F Bánhidy, F Mockenhaupt, FP Mockenhaupt, FP Mockenhaupt, G Snounou, H Reyburn, H Verhoef, HL Guyatt, IA McGregor, J Baker, J Lusingu, J Wooden, JM Tham, John PA Lusingu, JP Lusingu, JR Forney, K Källander, KA Bojang, LC Okell, LEG Mboera, LM Singer, M Amexo, M Dhorda, M Fried, M Fried, M Mayxay, M Mayxay, Martha Lemnge, Mayke Oesterholt, Michael Alifrangis, ML McMorrow, N Othnigué, NEER Singh, NJ White, P Bejon, P Mens, P Mens, Pamela A Magistrado, PE Duffy, Philippe Deloron, R Bǿdker, RW Steketee, S Gerstl, S Karl, S Schachterle, Stéphanie Boström, T Jelinek, TA VanderJagt, The United Republic of Tanzania, Thor Theander, TT Thomsen, V Batwala, WHO   +77 more
core   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR

Virology Journal, 2010
Background Human cytomegalovirus (HCMV) infection poses a significant health threat to immunocompromised individuals. Here we performed this study to set up a highly sensitive nested PCR method applicable for detecting HCMV infection in high-risk ...
Zhou Yi-Hua, Zhang Shu, Li Lei, Hu Yali
doaj   +1 more source

Evaluation of Nested PCR in Diagnosis of Typhoid Fever [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal ...
Pradyot, Prakash, Om Prakash, Mishra, Alok Kumar, Singh, Anil Kumar, Gulati, Gopal, Nath   +4 more
openaire   +2 more sources

ELABELA Targets Mitochondria to Modulate Heart Development

Advanced Science, EarlyView.
The role of peptide ELABELA (ELA) in cardiomyocyte apoptosis and congenital heart disease (CHD) is unclear. ELA deficiency caused cardiomyocyte apoptosis and CHD. A novel ELA‐APJ‐AKT‐BCL2/BAX axis in regulating mitochondrial function and contributing to CHD pathogenesis was established.
Jian Wang, Qingjie Wang, Zhikang Xu, Shuang Zhou, Yue Zhou, Junjie Yang, Lingfeng Tong, Zhuo Meng, Mei Yang, Wen Zhao, Tie Yang, Hualin Wang, Jun Zhang, Rubin Tan, Lei Wang, Yuqiang Huang, Bin Zhou, Sun Chen, Bing Zhang, Jinxiang Yuan, Jianyuan Zhao, Alex F. Chen, Kun Sun   +22 more
wiley   +1 more source

Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]

, 2012
Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi, Coates, Craig J, Dang, Nong C, Maragathavally, Kommineni J, Moisyadi, Stefan, Owens, Jesse B, Segal, David J, Stoytchev, Ilko, Stoytcheva, Zoia, Urschitz, Johann   +9 more
core   +5 more sources

Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi [PDF]

Pesquisa Veterinária Brasileira, 2011
Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T.
Leal, Danielle C., Madruga, Cláudio R., Matos, Paulo F. de, Souza, Bárbara M. P. da S., Franke, Carlos R.   +4 more
openaire   +4 more sources