Results 41 to 50 of about 97,450 (165)

Hierarchical DNA Memory Based on Nested PCR [PDF]

, 2003
This paper presents a hierarchical DNA memory based on nested PCR. Each DNA strand in memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers.
Satoshi Kashiwamura, Masahito Yamamoto, Atsushi Kameda, Toshikazu Shiba, Azuma Ohuchi   +4 more
openaire   +1 more source

Evaluation of Group specific Nested PCR for detection of Bluetongue virus [PDF]

Veterinary World, 2009
During the present study RT-PCR and nested PCR was evaluated for the detection of BTV in blood samples collected from suspected cases of bluetongue. Due to conserved nature, NS1 gene was targeted for the development of partial length and nested PCR assay.
Chauhan H C, Kher H N, Chandel B S, Dadawala A I, Jain L.,Agrawal S M and Bhadaniya A   +4 more
doaj  

Detection of Candida albicans by Nested PCR

Journal of the Japanese Association for Infectious Diseases, 1994
A nested PCR assay was developed for the diagnosis of Candida albicans infection. A segment of the C. albicans beta-tubulin gene was amplified in this assay that was sensitive with the detection limit of 10 fg DNA of C. albicans. This assay was found to be specific by obtaining the negative results with 13 organisms (5 other Candida, 2 Cryptococcus, 3 ...
openaire   +3 more sources

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +1 more source

Nested RT-PCR for ante mortem diagnosis of rabies from body secretion/excretion of animals suspected for rabies

Veterinary World, 2012
Aim: The present study deals with molecular technique Nested RT-PCR for detection of rabies viral RNA from biological fluid samples (Saliva, Milk and Urine) collected from animal suspected for rabies and to compare the sensitivity of Nested RT-PCR ...
M Dandale, C K Singh, V Ramneek, D Deka, B S Sandhu, K Bansal, N K Sood   +6 more
doaj   +1 more source

Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

Malaria Journal, 2012
Background A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes.
Lee Pei-Wen, Ji Dar-Der, Liu Chia-Tai, Rampao Herodes S, do Rosario Virgilio E, Lin I-Feng, Shaio Men-Fang   +6 more
doaj   +1 more source

Leptospirosis diagnosis using Nested-PCR [PDF]

Brazilian Journal of Microbiology, 2003
Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods.
Nassi, Fernanda, Seixas, Fabiana Kömmling, Jouglard, Sandra Denize Dorneles, Simionatto, Simone, Silva, Everton Fagonde, Seyffert, Núbia, Brod, Claudiomar Soares, Dellagostin, Odir Antonio   +7 more
openaire   +3 more sources

Performance of nested-RT-PCR assays for Zika and Dengue detection

Acta Scientiarum: Biological Sciences
Arboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses.
José Henrique Francisco Roma, Rachel Cruz Alves, Stefhano Luis Candido, Valéria Dutra, Luciano Nakazato, Renata Dezengrini-Slhessarenko, Juliana Helena Chavez-Pavoni, Mariângela Ribeiro Resende   +7 more
doaj   +1 more source

Nested RT-PCR in a Single Closed Tube

, 2003
There are several methods now in widespread use for detecting and characterizing specific RNA targets. These methods include in situ hybridization, Northern blotting, dot or slot blot, RNase protection assay, and reverse transcription coupled to polymerase chain reaction (RT-PCR).
Olmos, Antonio, Esteban, Olga, Bertolini, Edson, Cambra, Mariano   +3 more
openaire   +3 more sources