Results 61 to 70 of about 188,318 (309)

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Nested PCR espécie-específica para o diagnóstico da infecção por Brucella ovis em carneiros

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj  

Detection of Pneumocystis in lungs of bats from Brazil by PCR amplification Detecção de Pneumocystis em pulmões de morcegos no Brasil por Nested-PCR

Pesquisa Veterinária Brasileira, 2009
Pneumocystis has been isolated from a wide range of unrelated mammalian hosts, including humans, domestic and wild animals. It has been demonstrated that the genome of Pneumocystis of one host differs markedly from that of other hosts. Also, variation in
Edna Maria Cavallini Sanches, Susi M. Pacheco, Alison S. Cericatto, Rosane M. Melo, Edson Molleta Colodel, Jennifer Hummel, Simone P. Bianchi, Andréia Spanamberg, Janio M. Santurio, Laerte Ferreiro   +9 more
doaj   +1 more source

A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels

Frontiers in Microbiology, 2022
Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii.
Susana Ruiz-Ruiz, Susana Ruiz-Ruiz, Carolina A. Ponce, Nicole Pesantes, Nicole Pesantes, Rebeca Bustamante, Gianna Gatti, Viviana San Martin, Mireya Gutierrez, Pamela Bórquez, Sergio L. Vargas, Fabien Magne, Enrique J. Calderón, Enrique J. Calderón, Vicente Pérez-Brocal, Vicente Pérez-Brocal, Andrés Moya, Andrés Moya, Andrés Moya   +18 more
doaj   +1 more source

Cryptosporidium infection in patients with gastroenteritis in Sari, Iran [PDF]

, 2014
Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries.
Ahmadpour, E., Gholami, S., Khanmohammadi, M., Nakhjiri, S.K., Paqhe, E.S., Ramazannipour, H., Shahbazi, A.   +6 more
core  

Tumor‐Derived LAMB3 Drives Immunosuppressive LRRC15+ Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development

Advanced Science, EarlyView.
A single‐cell atlas of pancreatic ductal adenocarcinoma development reveals progressive ductal‐fibroblast‐immune crosstalk. Tumor‐derived LAMB3 drives the formation of immunosuppressive LRRC15+ fibroblasts through the ITGB1/FAK/MAPK/FOSL2 signaling. Glycolytic reprogramming upregulates LAMB3 and correlates with LRRC15+ fibroblast enrichment.
Xuqing Shi, Hangqi Liu, Jianru Sun, Xiaoding Liu, Xinping Jv, Longyun Chen, Yuhan Zhang, Hui Zhang, Xudong Xing, Ruiyu Li, Xinyi Ke, Jun Wang, Xianglin Yin, Bohan Liu, Qixian Liu, Yuan Wang, Junliang Lu, Shiyi Liu, Junyi Pang, Yumeng Cai, Menghua Dai, Fan Bai, Huanwen Wu, Zhiyong Liang   +23 more
wiley   +1 more source

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

BMC Infectious Diseases, 2005
Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M, Volckaert Guido, Messmer Trudy O, Verminnen Kristel, Van Loock Marnix, Vanrompay Daisy   +5 more
doaj   +1 more source

Distribution and prevalence of malaria parasites among long-tailed macaques (Macaca fascicularis) in regional populations across Southeast Asia. [PDF]

, 2016
BackgroundPlasmodium knowlesi and Plasmodium cynomolgi are two malaria parasites naturally transmissible between humans and wild macaque through mosquito vectors, while Plasmodium inui can be experimentally transmitted from macaques to humans.
Du, Hongli, Houghton, Paul, Kadir, Khamisah Abdul, Quintanilla-Zariñan, Leslie Fabiola, Singh, Balbir, Smith, David Glenn, Villano, Jason, Zhang, Xinjun   +7 more
core   +1 more source

HNRNPU K181 Lactylation Drives Cervical Cancer Growth by Upregulating PHGDH and Reprogramming Serine Metabolism

Advanced Science, EarlyView.
Lactate in cervical cancer induces HNRNPU K181 lactylation, opposed by NAA50‐mediated acetylation and suppressed by Pazopanib. This lactylation enhances HNRNPU binding to PHGDH pre‐mRNA exon 1, maintaining exon 1‐containing transcripts and mRNA stability, thereby activating serine metabolism.
Chang Zhang, Qingfei Meng, Hui Jiao, Huixin Liu, Xiangchao Wang, Honglan Zhou, Yishu Wang   +6 more
wiley   +1 more source

HNRNPD Induces Radioresistance in Nasopharyngeal Carcinoma by Sequestering GRAMD4 mRNA in Stress Granules

Advanced Science, EarlyView.
HNRNPD promotes radioresistance in nasopharyngeal carcinoma by enhancing stress granule assembly and sequestering GRAMD4 mRNA. This suppresses GRAMD4 translation and inhibits mitochondrial apoptosis. Targeting the integrated stress response with ISRIB restores GRAMD4 expression and sensitizes tumors to radiotherapy, revealing a translational control ...
Yingzi Li, Tong Xiang, Yuanyuan Liu, Desheng Weng, Jingjing Zhao, Hao Chen, Yan Tang, Songzuo Xie, Hao Zhang, Jun Luo, Xinyi Yang, Qiuzhong Pan, Muping Di, Jianchuan Xia   +13 more
wiley   +1 more source

Evaluation of Group specific Nested PCR for detection of Bluetongue virus [PDF]

Veterinary World, 2009
During the present study RT-PCR and nested PCR was evaluated for the detection of BTV in blood samples collected from suspected cases of bluetongue. Due to conserved nature, NS1 gene was targeted for the development of partial length and nested PCR assay.
Chauhan H C, Kher H N, Chandel B S, Dadawala A I, Jain L.,Agrawal S M and Bhadaniya A   +4 more
doaj