Results 51 to 60 of about 188,318 (309)

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

Mechanistic target of rapamycin (mTOR) signaling genes in decapod crustaceans: cloning and tissue expression of mTOR, Akt, Rheb, and S6 kinase in the green crab, Carcinus maenas, and blackback land crab, Gecarcinus lateralis [PDF]

, 2014
Mechanistic target of rapamycin (mTOR) controls global translation of mRNA into protein by phosphorylating p70 S6 kinase (S6K) and eIF4E-binding protein-1. Akt and Rheb, a GTP-binding protein, regulate mTOR protein kinase activity. Molting in crustaceans
Abuhagr, Ali M., Chang, Ernest S., MacLea, Kyle S., Mykles, Donald L.   +3 more
core   +2 more sources

Hierarchical DNA Memory Based on Nested PCR [PDF]

, 2003
This paper presents a hierarchical DNA memory based on nested PCR. Each DNA strand in memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers.
Satoshi Kashiwamura, Masahito Yamamoto, Atsushi Kameda, Toshikazu Shiba, Azuma Ohuchi   +4 more
openaire   +1 more source

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Bovine Leukaemia Virus Tax Antigen Identification in Human Lymphoma Tissue: Possibility of onco-protein Gene Ttransmission

Research in Molecular Medicine, 2019
Background: Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from
Camellia Taghadosi, Gholamali Kojouri, Alimohammad Ahadi, Mohammad Hashemi Bahremani, Abdolnabi Kojouri   +4 more
doaj  

Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

Revista Brasileira de Parasitologia Veterinária, 2018
This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo, Bruna Nicoleti Santana, Elis Domingos Ferrari, Alex Akira Nakamura, Walter Bertequini Nagata, Ana Rita Moraes Nardi, Marcelo Vasconcelos Meireles   +6 more
doaj   +1 more source

Toxoplasma Gondii Identification in Mother\u27s Blood and Fetal Tissue with Nested PCR [PDF]

, 2006
Toxoplasma gondii identification in mother’s blood and fetal tissue with nested PCRObjective: To examine the correlation between Toxoplasma gondii infection with spontaneous abortion on pregnant women based on nested PCR result from mother’s ...
Imada, M. (Mihoko), Masengi-Rumopa, J. A. (Juliana), Rahardjo, T. M. (Theresia), Suryawan, A. (Aloysius), Tuda, J. S. (Josef)   +4 more
core  

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]

, 2019
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els, Hardy, Jarryt, Hebberecht, Laura, Mortier, Virginie, Schauvliege, Marlies, Staelens, Delfien, Vancoillie, Leen, Verhofstede, Chris   +7 more
core   +1 more source

Detection of Candida albicans by Nested PCR

Journal of the Japanese Association for Infectious Diseases, 1994
A nested PCR assay was developed for the diagnosis of Candida albicans infection. A segment of the C. albicans beta-tubulin gene was amplified in this assay that was sensitive with the detection limit of 10 fg DNA of C. albicans. This assay was found to be specific by obtaining the negative results with 13 organisms (5 other Candida, 2 Cryptococcus, 3 ...
openaire   +3 more sources

Circular RNA circNrip1 Interacts with SYNCRIP to Promote Neuropathic Pain by Stabilizing Tlr2 mRNA in Primary Sensory Neurons

Advanced Science, EarlyView.
Model of circNrip1 (cNrip1) upregulation driving neuropathic pain mechanisms. After peripheral nerve injury, increased FUS triggers the formation and upregulation of cNrip1 in injured DRG neurons. Upregulated cNrip1 recruits SYNCRIP to the 3′‐UTR of Tlr2 mRNA by binding to both, thereby promoting SYNCRIP‐triggered Tlr2 mRNA stability and increasing ...
Xiaozhou Feng, Jifang Xiao, Congcong Huang, Bao‐Chun Jiang, Bing Wang, Xu Li, Shibin Du, Tolga Berkman, Chun‐Hsien Wen, Weihua Cai, Dilip Sharma, Kun Wang, Longfei Ma, Huijuan Hu, Yuan‐Xiang Tao   +14 more
wiley   +1 more source