Results 51 to 60 of about 97,450 (165)

Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical, 2007
A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR) foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do ...
Karla Valéria Batista Lima, Maria Luíza Lopes, Edvaldo Carlos Brito Loureiro, Maurimélia Mesquita Costa, Ninarosa Calzavara Cardoso, George Leandro Ferreira Lima, Maísa Silva Sousa   +6 more
doaj   +1 more source

Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge

Archives of Environmental Protection, 2014
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra, Wiszniowski Jarosław, Ciesielski Sławomir   +2 more
doaj   +1 more source

Detection of cytomegalovirus using ‘boosted’ nested PCR

Molecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg, S A, Nordbø, P, Winge, A, Dalen   +3 more
openaire   +2 more sources

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquímica e nested-PCR

Revista Brasileira de Parasitologia Veterinária, 2009
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB).
Aline Diniz Cabral, Clarice Neves Camargo, Nara Thiers Cacciatori Galleti, Liria Hiromi Okuda, Edviges Maristela Pituco, Claudia Del Fava   +5 more
doaj   +1 more source

Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Universa Medicina, 2007
Latar Belakang DNA rpob (RNA polymerase sub unit b) Mycobacterium tuberculosis dapat diamplifikasi secara spesifik dengan metode nested polymerase chain reaction (PCR).
Maria Lina Rosilawati
doaj   +1 more source

Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome

Memorias do Instituto Oswaldo Cruz, 2004
We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS ...
Marcos Lázaro Moreli, Ricardo Luiz Moro de Sousa, Luiz Tadeu Moraes Figueiredo   +2 more
doaj   +1 more source

Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology, 2006
world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several ...
Nastiti Wijayanti, Hera Nirwati, Tri Wibawa, Aris Haryanto, S. Sutaryo   +4 more
doaj   +1 more source

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source

Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA)

Jurnal Veteriner, 2014
Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed
Trioso Purnawarman, I Wayan Teguh Wibawan, Fachriyan Hasmi Pasaribu, Agus Setiyono, Muharam Saepulloh   +4 more
doaj  

Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas A nested-PCR protocol for detection of the chicken anemia virus

Pesquisa Veterinária Brasileira, 2005
Este trabalho descreve o estabelecimento de um pro!tocolo de "nested-PCR" para a detecção do vírus da anemia das galinhas (CAV, chicken anemia virus), agente causador da anemia infecciosa das galinhas. Para a extração de DNA a partir de amostras clínicas
Simone Simionatto, Carlos André da Veiga Lima-Rosa, Lauricio Librelotto Rubin, Cláudio Wageck Canal   +3 more
doaj   +1 more source