Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques [PDF]
Scientific ReportsAcanthamoeba keratitis (AK) is a severe eye infection that can cause severe vision loss, especially in contact lens wearers. Rapid and accurate diagnosis is crucial for achieving favorable outcomes. Traditional methods, such as culture, are slow and have
Sarah Khaksar +13 more
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Molecular identification of Anaplasma platys in cattle by nested PCR. [PDF]
Iran J Microbiol, 2023 Background and Objectives: Anaplasmosis is a zoonotic disease caused by Gram- negative bacterium from Anaplasmata- ceae family. Anaplasma causes high economic losses worldwide. 16S rRNA analysis was used to diagnose Anaplasma platys in Cattle. Phylogenetic tree and estimation of evolutionary divergence between A.
Al-Saadi M, Al-Sallami D, Alsultan A.
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Development and Evaluation of a Dual-Target One-Step Nested PCR for the Detection of Spotted Fever Group Rickettsia spp. in Ticks [PDF]
PathogensSpotted fever group (SFG) rickettsioses are tick-borne infectious diseases caused by more than 30 Rickettsia species. As ticks may harbor and transmit multiple pathogens during a single blood meal, sensitive and specific molecular detection methods are ...
Phiaw Chong Foo +4 more
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Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis [PDF]
Archives of Razi Institute, 2021 Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections.
L Rahbarnia +3 more
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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples [PDF]
Revista Brasileira de Parasitologia Veterinária, 2022 The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds.
Bruna Nicoleti Santana +4 more
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Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets. [PDF]
PLoS ONE, 2015 Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences.
Guoqin Yu +4 more
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Nested PCR for Diagnosis of Typhoid Fever [PDF]
Journal of Clinical Microbiology, 2005 The superiority of the nested PCR employing H 1-d primers over culture or single-dilution Widal (4) should be reevaluated for isolates from unusual sites and from among those coinfected with human immunodeficiency virus (HIV)/AIDS. Numerous extraintestinal complications are known with Salmonella enterica serovar Typhi infection.
Subhash C, Arya +4 more
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APPLICATION OF NESTED REVERSE TRANSCRIPTION PCR FOR DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN SEMEN [PDF]
Veterinary Medical Journal - Giza, 2003 A nested reverse transcription-polymerase chain reaction (RT-PCR) was employed for detection of bovine viral diarrhea virus (BVDV) in 59 semen samples collected from farms of known history of BVDV infection. The results indicated that 6 (10.2%) and 8 (13.
A AMIN +2 more
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Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]
Journal of Clinical Microbiology, 1998 ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F. +6 more
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Research in Plant Disease, 2015 The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim +4 more
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