Results 41 to 50 of about 1,037,260 (308)

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Molecular Genotyping of HBV by using Nested PCR-RFLP among Hepatitis B Patients in Daerah Istimewa Yogyakarta Province and Surrounding Area

Indonesian Journal of Biotechnology, 2008
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto, Nenny Sri Mulyani, Titis Widowati, Nastiti Wijayanti, Purnomo Hadi   +4 more
doaj   +1 more source

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

Advanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang, Qiang Chen, Aihua Jin, Xiaoqi Yang, Hui Zhou, Weiquan Gong, Jin Cheng, Jun Dai, Minjin Wang, Meihua Piao, Yuanyi Wang, Nan Zhou   +11 more
wiley   +1 more source

Sequences of nested PCR products-SVNV-06302015

, 2016
Sequences of typical Nested PCR ...
Dasgupta, Ranjit, Daren Mueller (846381), Mueller, Daren, Groves, Carol, German, Thomas, Carol Groves (846379), Smith, Damon L., Ranjit Dasgupta (3242829), Thomas German (846380), Damon L. Smith (846382)   +9 more
core   +1 more source

Gestational Hypoxia Disrupts Medial Ganglionic Eminence Progenitor Dynamics and Interneuron Development in Schizophrenia

Advanced Science, EarlyView.
FES‐derived MGE spheroids exhibit progenitor‐stage alterations in developmental trajectory and hypoxia‐responsive transcriptional programs, followed by functional disruption. Gestational hypoxia recapitulates impaired progenitor proliferation, shortened cell‐cycle progression, interneuron developmental abnormalities, and schizophrenia‐like behaviors in
Peiyan Ni, Qiancheng Zhang, Youhui Jiang, Huiying Yao, Chuqing Zhou, Xiaoyi Tian, Shiyingnan Gao, Jun Zhao, Xinyi Ren, Naxin Xu, Mengshan Fu, Liansheng Zhao, Xueyu Qi, Xueli Yu, Wanjun Guo, Sangmi Chung, Fan Guo, Tao Li   +17 more
wiley   +1 more source

Detection of Pneumocystis in lungs of bats from Brazil by PCR amplification Detecção de Pneumocystis em pulmões de morcegos no Brasil por Nested-PCR

Pesquisa Veterinária Brasileira, 2009
Pneumocystis has been isolated from a wide range of unrelated mammalian hosts, including humans, domestic and wild animals. It has been demonstrated that the genome of Pneumocystis of one host differs markedly from that of other hosts. Also, variation in
Edna Maria Cavallini Sanches, Susi M. Pacheco, Alison S. Cericatto, Rosane M. Melo, Edson Molleta Colodel, Jennifer Hummel, Simone P. Bianchi, Andréia Spanamberg, Janio M. Santurio, Laerte Ferreiro   +9 more
doaj   +1 more source

Outer primers sequence for nested PCR.

, 2016
Outer primers sequence for nested PCR.
Barbara J. Morley (2541169), Stéphanie St-Pierre (2541181), Alain R. Simard (2541166), Camille Champigny (2541178), Éric LeBlanc (2541172), Wei Jiang (14986), Patrick Roy (2541175), Junwei Hao (324277)   +7 more
core   +1 more source

Multi‐Omics Integration Identifies a CDH3‐Associated Malignant Epithelial State and Immunosuppressive Niche to Predict Prognosis in Thymic Epithelial Tumors

Advanced Science, EarlyView.
Single‐cell, spatial, molecular, and pathology analyses identify a CDH3‐associated malignant epithelial state in thymic epithelial tumors. This state links stem‐like and EMT programs to M2 macrophage–rich immunosuppressive niches, genomic instability, poor survival, and drug vulnerability.
Yuntao Feng, Jingyu Chen, Lang Xia, Tao Wang, Yuzhou Wang, Lei Zhang, Guofang Zhao, Long Xu, Juemin Yu, Yunlang She, Junqi Wu, Yue Zhao, Chang Chen, Deping Zhao   +13 more
wiley   +1 more source

Bovine Leukaemia Virus Tax Antigen Identification in Human Lymphoma Tissue: Possibility of onco-protein Gene Ttransmission

Research in Molecular Medicine, 2019
Background: Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from
Camellia Taghadosi, Gholamali Kojouri, Alimohammad Ahadi, Mohammad Hashemi Bahremani, Abdolnabi Kojouri   +4 more
doaj  

NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF HELICOBACTER PYLORI IN GASTRIC BIOPSY SPECIMENS

, 2020
. Sensitivity and specificity are important for tests used to defect Helicobacter pylori infection from gastric biopsy specimens. Molecular methods, such as PCR and nested PCR, are sensitive methods for H. pylori detection.
Nested Pcr For H, Pylori, Detection
core