Results 41 to 50 of about 97,549 (263)

HNRNPU K181 Lactylation Drives Cervical Cancer Growth by Upregulating PHGDH and Reprogramming Serine Metabolism

Advanced Science, EarlyView.
Lactate in cervical cancer induces HNRNPU K181 lactylation, opposed by NAA50‐mediated acetylation and suppressed by Pazopanib. This lactylation enhances HNRNPU binding to PHGDH pre‐mRNA exon 1, maintaining exon 1‐containing transcripts and mRNA stability, thereby activating serine metabolism.
Chang Zhang, Qingfei Meng, Hui Jiao, Huixin Liu, Xiangchao Wang, Honglan Zhou, Yishu Wang   +6 more
wiley   +1 more source

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Nested PCR espécie-específica para o diagnóstico da infecção por Brucella ovis em carneiros

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj  

Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

Revista Brasileira de Parasitologia Veterinária, 2018
This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo, Bruna Nicoleti Santana, Elis Domingos Ferrari, Alex Akira Nakamura, Walter Bertequini Nagata, Ana Rita Moraes Nardi, Marcelo Vasconcelos Meireles   +6 more
doaj   +1 more source

A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels

Frontiers in Microbiology, 2022
Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii.
Susana Ruiz-Ruiz, Susana Ruiz-Ruiz, Carolina A. Ponce, Nicole Pesantes, Nicole Pesantes, Rebeca Bustamante, Gianna Gatti, Viviana San Martin, Mireya Gutierrez, Pamela Bórquez, Sergio L. Vargas, Fabien Magne, Enrique J. Calderón, Enrique J. Calderón, Vicente Pérez-Brocal, Vicente Pérez-Brocal, Andrés Moya, Andrés Moya, Andrés Moya   +18 more
doaj   +1 more source

Xenogeneic Mitochondrial Transplantation Improves Selected Age‐Associated Phenotypes in Mice

Advanced Science, EarlyView.
Yak‐derived xenogeneic mitochondrial transplantation improves selected age‐associated phenotypes in mice, enhances mitochondrial functional readouts, and engages host mitochondrial quality‐control pathways. Broad tissue biodistribution, increased ATP production and mtDNA copy number, reduced ROS levels and dysfunctional mitochondria, improved motility ...
Wenpeng Li, Xijia Wang, Yaning Hu, Zhen Yang, Tian Niu, Xingbo Zhao   +5 more
wiley   +1 more source

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

BMC Infectious Diseases, 2005
Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M, Volckaert Guido, Messmer Trudy O, Verminnen Kristel, Van Loock Marnix, Vanrompay Daisy   +5 more
doaj   +1 more source

Detection of Candida albicans by Nested PCR

Journal of the Japanese Association for Infectious Diseases, 1994
A nested PCR assay was developed for the diagnosis of Candida albicans infection. A segment of the C. albicans beta-tubulin gene was amplified in this assay that was sensitive with the detection limit of 10 fg DNA of C. albicans. This assay was found to be specific by obtaining the negative results with 13 organisms (5 other Candida, 2 Cryptococcus, 3 ...
openaire   +3 more sources

Detection of cytomegalovirus using ‘boosted’ nested PCR

Molecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg, S A, Nordbø, P, Winge, A, Dalen   +3 more
openaire   +2 more sources

CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell Carcinoma

Advanced Science, EarlyView.
This study reveals a metabolic signaling axis in the OSCC microenvironment where palmitic acid (PA) drives the activation of CAFs. PA uptake triggers CEBPG‐dependent epigenetic remodeling to upregulate ERN1 and TMBIM6, thereby mitigating ER stress. This adaptive program sustains CAF survival and the pro‐metastatic phenotype, establishing this pathway ...
Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shang   +6 more
wiley   +1 more source

Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients Detecção de herpesvirus humano-7 por nested-PCR qualitativo: comparação entre indivíduos sadios e receptores de transplante hepático

Revista da Sociedade Brasileira de Medicina Tropical, 2008
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host.
Ronaldo Luis Thomasini, Juliana de Moraes Martins, Daniela Corte Parola, Sandra Helena Alves Bonon, Ilka de Fátima Santana Ferreira Boin, Luis Sérgio Leonardi, Marília Leonardi, Sandra Cecília Botelho Costa   +7 more
doaj   +1 more source