Results 31 to 40 of about 986,696 (337)

Single-tube nested PCR in the diagnosis of tuberculosis. [PDF]

Journal of Clinical Pathology, 1996
AIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to
Chan, KS, Yuen, KY, Ng, MH, Ng, WF, Chan, CM, Yim, KHM, Yam, WC   +6 more
openaire   +3 more sources

Nested PCR for the detection of Candidatus arthromitus in fish [PDF]

FEMS Microbiology Letters, 2010
Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and release strings of endospores in the ...
CECCHINI Francesca, COMI, Giuseppe, FONTANILLAS R., GIUSTO Cristina, IACUMIN, Lucilla, MANZANO, Marisa, PATTHEY Chiara   +6 more
openaire   +2 more sources

Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

Kufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi, Baqur A. Sultan, Muhsin A. Al-Dhalimi, Thikra A. Mahmood   +3 more
doaj   +1 more source

Detection of toxoplasmosis by serology and nested-PCR in kidney transplant recipients and patients on hemodialysis from Sri Lanka. [PDF]

BMC Infect Dis
Weerasooriya G, Manamperi A, Banneheke B.   +2 more
europepmc   +3 more sources

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj   +1 more source

Determination of Cryptosporidium spp. in Van cats by nested PCR [PDF]

Medycyna Weterynaryjna, 2018
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in
Özkan , Cumali, Denizhan, Vural, Yılmaz, Ali Bilgin, Göz, Yaşar, Orunçkılınç, Özlem   +4 more
openaire   +4 more sources

COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]

Acta Medica Iranica, 1997
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1, A.A. Velayati, M. Bahadori, M. Masjedi   +3 more
doaj   +2 more sources

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

PCR analysis of the presence and location of Mycobacterium avium in a constructed reed bed, with implications for avian tuberculosis control [PDF]

, 2009
The potential of reed beds to act as biofilters of pathogenic and environmental mycobacteria was investigated through examination of the fate of mycobacteria in a constructed reed bed filtering effluent from a large captive wildfowl collection ...
ZSL, Mwangi, D, Donoghue, H D, Wildfowl And Wetlands Trust, Cromie, RL, Drewe, JA, Donoghue, HD, Drewe, J A, Cromie, R L, UCL   +9 more
core   +1 more source

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

BMC Infectious Diseases, 2004
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria, Giuliani Giuseppe, De Vecchi Elena, Lombardi Alessandra, Drago Lorenzo, Gismondo Maria   +5 more
doaj   +1 more source