Results 31 to 40 of about 1,037,260 (308)

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source

Primers used for nested PCR.

, 2013
Primers used for nested PCR.
Vinaya Ramanabhiraman (287806), Anjali Shetty (287809), Camilla Rodrigues (287811), Mubin Kazi (287808), Chaitali Nikam (287803), Manoj Mulakkapurath Narayanan (287805), Manjula Jagannath (287804)   +6 more
core   +1 more source

Hierarchical DNA Memory Based on Nested PCR [PDF]

, 2003
This paper presents a hierarchical DNA memory based on nested PCR. Each DNA strand in memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers.
Satoshi Kashiwamura, Masahito Yamamoto, Atsushi Kameda, Toshikazu Shiba, Azuma Ohuchi   +4 more
openaire   +2 more sources

A comparative study on detection of bartonella henselae infection by culture followed by pcr, nested-pcr and ifa [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017
Cats are the main reservoirs of zoonotic Bartonella henselae which are the causative agents of Cat Scratch Disease (CSD). The aim of this study is to compare three diagnostic methods including culture followed by PCR from whole blood, nested-PCR from ...
DIREN SIGIRCI B, CELIK B, BASARAN KAHRAMAN B, GUMUS B, METINER K, ADIGUZEL MC, IKIZ S, BAGCIGIL AF, OZGUR NY, AK S   +9 more
doaj   +1 more source

Leptospirosis diagnosis using Nested-PCR [PDF]

Brazilian Journal of Microbiology, 2003
Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods.
Nassi, Fernanda, Seixas, Fabiana Kömmling, Jouglard, Sandra Denize Dorneles, Simionatto, Simone, Silva, Everton Fagonde, Seyffert, Núbia, Brod, Claudiomar Soares, Dellagostin, Odir Antonio   +7 more
openaire   +3 more sources

Detection of DENV strains by RT-PCR and nested PCR.

, 2018
(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/TS4 primers to detect DENV1, DENV3 and DENV4, respectively.
Michaël Bekaert (121005), Etienne Simon-Loriere (140017), Mohammed Bakheit (4960669), Cheikh Fall (5309297), Manfred Weidmann (448254), Benjamin Lopez-Jimena (4854436), Veasna Duong (106893), Sieghard Frischmann (710564), Amadou Alpha Sall (169451), Louis Lambrechts (241741), Oumar Faye (32488), Philippe Dussart (181796), Graham Harold (73273), Pranav Patel (415128)   +13 more
core   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method

Microorganisms
Spring viremia of carp (SVC) is a highly contagious disease that affects cyprinids, resulting in systemic hemorrhage, abdominal distension, exophthalmia, and high mortality in juveniles.
Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon   +6 more
doaj   +1 more source

Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR

Virology Journal, 2010
Background Human cytomegalovirus (HCMV) infection poses a significant health threat to immunocompromised individuals. Here we performed this study to set up a highly sensitive nested PCR method applicable for detecting HCMV infection in high-risk ...
Zhou Yi-Hua, Zhang Shu, Li Lei, Hu Yali
doaj   +1 more source

Primers using sequencing for nested PCR.

, 2018
Primers using sequencing for nested PCR.
Yun-Shien Lee (90565), Yi-Chun Chen (189669), Yu-hua Huang (4884958), Chiung-Mei Chen (188919), Kuo-Hsuan Chang (188893), Huei-Wen Chen (346111)   +5 more
core   +1 more source