Results 11 to 20 of about 97,549 (263)

Development of Nested-PCR Assay to Detect Acidovorax citrulli, a Causal Agent of Bacterial Fruit Blotch at Cucurbitaceae

Research in Plant Disease, 2015
The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim, Kyoung-Soo Park, Hye-Seong Kim, Hyok-In Lee, Jae-Soon Cha   +4 more
doaj   +1 more source

Single-tube nested PCR in the diagnosis of tuberculosis. [PDF]

Journal of Clinical Pathology, 1996
AIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to
Chan, KS, Yuen, KY, Ng, MH, Ng, WF, Chan, CM, Yim, KHM, Yam, WC   +6 more
openaire   +3 more sources

One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples

Frontiers in Veterinary Science, 2020
Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang, Joong Ki Song, Seongho Shin, Ki Myung Choi, Hyunil Kim   +4 more
doaj   +1 more source

Nested PCR for the detection of Candidatus arthromitus in fish [PDF]

FEMS Microbiology Letters, 2010
Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and release strings of endospores in the ...
CECCHINI Francesca, COMI, Giuseppe, FONTANILLAS R., GIUSTO Cristina, IACUMIN, Lucilla, MANZANO, Marisa, PATTHEY Chiara   +6 more
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An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Revista da Sociedade Brasileira de Medicina Tropical, 2013
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fabio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson Jose Venancio   +6 more
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Nested PCR Analysis of Plasmodium Parasites [PDF]

, 2003
In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire   +2 more sources

A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

Revista da Sociedade Brasileira de Medicina Tropical, 2010
INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi, Débora Fonseca Vituri, Pedro Sebastião Raimundo Dionízio Filho, Alexandre Augusto Sasaki, Maria Sueli Soares Felipe, Emerson José Venancio   +5 more
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Evaluation of Nested PCR in Diagnosis of Typhoid Fever [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal ...
Pradyot, Prakash, Om Prakash, Mishra, Alok Kumar, Singh, Anil Kumar, Gulati, Gopal, Nath   +4 more
openaire   +2 more sources

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
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Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

Kufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi, Baqur A. Sultan, Muhsin A. Al-Dhalimi, Thikra A. Mahmood   +3 more
doaj   +1 more source