Results 21 to 30 of about 1,037,260 (308)
Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis
Kufa Journal for Nursing Sciences, 2015 Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi +3 more
doaj +1 more source
Single-tube nested PCR in the diagnosis of tuberculosis. [PDF]
Journal of Clinical Pathology, 1996 AIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to
Chan, KS +6 more
openaire +3 more sources
Nested PCR for the detection of Candidatus arthromitus in fish [PDF]
FEMS Microbiology Letters, 2010 Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and release strings of endospores in the ...
CECCHINI Francesca +6 more
openaire +2 more sources
Revista da Sociedade Brasileira de Medicina Tropical, 2010 INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi +5 more
doaj +1 more source
COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]
Acta Medica Iranica, 1997 A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1 +3 more
doaj +2 more sources
Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2022 The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA +2 more
doaj +1 more source
Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013 The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa +8 more
doaj +1 more source
Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Veterinary World, 2017 Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
doaj +1 more source
BMC Infectious Diseases, 2004 Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria +5 more
doaj +1 more source
Inner primers sequence nested PCR.
, 2016 Inner primers sequence nested PCR.
Barbara J. Morley (2541169) +7 more
core +1 more source