Results 21 to 30 of about 986,696 (337)

Comparative performance of microscopy, nested PCR, and real-time PCR for screening avian haemosporidian parasites in Afrotropical starlings (family Sturnidae)

, 2023
Prevalence studies of avian haemosporidian parasites frequently use microscopy and the nested polymerase chain reaction (PCR) protocols for detecting infections.
Ndlovu, Mduduzi, Wardjomto, Maliki B., Mduduzi Ndlovu, Pori, Tinotendashe, Nangammbi, Tshifhiwa C., Antón Pérez-Rodríguez, Tshifhiwa C. Nangammbi, Tinotendashe Pori, Wardjomto, Maliki, Nangammbi, Tshifhiwa, Maliki B. Wardjomto, Pérez-Rodríguez, Antón   +11 more
core   +1 more source

Correction: Utility of multi-target nested PCR and ELISPOT assays for the detection of paucibacillary leprosy: a possible conclusion of clinical laboratory misdiagnosis. [PDF]

Front Cell Infect Microbiol
Jiang H, Shi Y, Chokkakula S, Zhang W, Long S, Wang Z, Kong W, Long H, Wu L, Hu L, Yao Q, Wang H.   +11 more
europepmc   +3 more sources

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller, Ganesh Srinivasamoorthy, Venkatachalam Udhayakumar, John W. Barnwell, Leopoldo Villegas, S. Patrick Kachur, Demas, Allison, Kissinger, Jessica C, Villegas, Leopoldo, Abdunoor M. Kabanywanyi, Jessica C. Kissinger, Deborah Sumari, Escalante, Ananias A, Lucchi, Naomi W, Udhayakumar, Venkatachalam, Sumari, Deborah, Peterson, David S, Ananias A. Escalante, Naomi W. Lucchi, Jeremy DeBarry, DeBarry, Jeremy, David S. Peterson, Oberstaller, Jenna, Allison Demas, Srinivasamoorthy, Ganesh, Kachur, S Patrick, Kabanywanyi, Abdunoor M, Barnwell, John W   +27 more
core   +1 more source

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases, 2011
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD   +4 more
doaj   +1 more source

Development of Nested-PCR Assay to Detect Acidovorax citrulli, a Causal Agent of Bacterial Fruit Blotch at Cucurbitaceae

Research in Plant Disease, 2015
The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim, Kyoung-Soo Park, Hye-Seong Kim, Hyok-In Lee, Jae-Soon Cha   +4 more
doaj   +1 more source

An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Revista da Sociedade Brasileira de Medicina Tropical, 2013
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fabio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson Jose Venancio   +6 more
doaj   +1 more source

One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples

Frontiers in Veterinary Science, 2020
Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang, Joong Ki Song, Seongho Shin, Ki Myung Choi, Hyunil Kim   +4 more
doaj   +1 more source

Nested PCR Analysis of Plasmodium Parasites [PDF]

, 2003
In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire   +2 more sources

Evaluation of Nested PCR in Diagnosis of Typhoid Fever [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal ...
Pradyot, Prakash, Om Prakash, Mishra, Alok Kumar, Singh, Anil Kumar, Gulati, Gopal, Nath   +4 more
openaire   +2 more sources

A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand [PDF]

, 2009
Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the
Thompson, R.C.A., K Darwin Murrell, Leelayoova, S, Murrell, K. Darwin, Cribb, Thomas, Cribb, T, Thomas Cribb, Cribb, T., Macaranas, J, Thompson R. C. Andrew, Julie Macaranas, Thompson, RCA, R C Andrew Thompson, Saovanee Leelayoova, Mungthin, M, Macaranas, Julie, Mungthin, M., Leelayoova, Saovanee, Thompson, R. C. Andrew, Macaranas Julie, Murrell K. Darwin, Traub, Rebecca J., Mungthin, Mathirut, Mathirut Mungthin, Traub, RJ, Murrell, KD, Cribb Thomas, Macaranas, J., Traub, R.J., Rebecca J Traub, Leelayoova, S., Traub Rebecca J., Mungthin Mathirut, Murrell, K.D., Leelayoova Saovanee   +34 more
core   +2 more sources