Determination of Cryptosporidium spp. in Van cats by nested PCR [PDF]
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in
Özkan , Cumali +4 more
openaire +4 more sources
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria +5 more
doaj +1 more source
Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]
The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA +2 more
doaj +1 more source
Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
doaj +1 more source
COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1 +3 more
doaj +2 more sources
Human papillomavirus detection and typing using a nested-PCR-RFLP assay
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser +4 more
doaj +1 more source
A comparative study on detection of bartonella henselae infection by culture followed by pcr, nested-pcr and ifa [PDF]
Cats are the main reservoirs of zoonotic Bartonella henselae which are the causative agents of Cat Scratch Disease (CSD). The aim of this study is to compare three diagnostic methods including culture followed by PCR from whole blood, nested-PCR from ...
DIREN SIGIRCI B +9 more
doaj +1 more source
Hierarchical DNA Memory Based on Nested PCR [PDF]
This paper presents a hierarchical DNA memory based on nested PCR. Each DNA strand in memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers.
Satoshi Kashiwamura +4 more
openaire +1 more source
Leptospirosis diagnosis using Nested-PCR [PDF]
Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods.
Nassi, Fernanda +7 more
openaire +3 more sources
Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source

