Results 51 to 60 of about 97,549 (263)

Evaluation of Pan-Dermatophyte Nested PCR in Diagnosis of Onychomycosis [PDF]

Journal of Clinical Microbiology, 2007
ABSTRACT In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene ( CHS1 ) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis.
Jaya, Garg, Ragini, Tilak, Sanjay, Singh, Anil Kumar, Gulati, Atul, Garg, Pradyot, Prakash, Gopal, Nath   +6 more
openaire   +2 more sources

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, EarlyView.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

Evaluation of Group specific Nested PCR for detection of Bluetongue virus [PDF]

Veterinary World, 2009
During the present study RT-PCR and nested PCR was evaluated for the detection of BTV in blood samples collected from suspected cases of bluetongue. Due to conserved nature, NS1 gene was targeted for the development of partial length and nested PCR assay.
Chauhan H C, Kher H N, Chandel B S, Dadawala A I, Jain L.,Agrawal S M and Bhadaniya A   +4 more
doaj  

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source

Machine Learning‐Enhanced Ultrasensitive Immuno‐CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma Biomarkers

Advanced Science, EarlyView.
This work presents a CRISPR‐based, ultrasensitive multiplex protein detection array capable of simultaneously analyzing six plasma biomarkers associated with Alzheimer's disease (AD). By integrating antibody‐mediated signal transduction, recombinase polymerase amplification, and spatially encoded CRISPR‐Cas12a, the system achieves detection sensitivity
Liding Zhang, Changwen Yang, Qian Yao, Xuewei Du, Shuai Ding, Yaoqiang Shi, Can Sheng, Ming Wang, Ying Han, Haiming Luo   +9 more
wiley   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

RNF138‐Mediated Ubiquitination and Degradation of NS5 Restricts Tick‐Borne Encephalitis Virus Infection

Advanced Science, EarlyView.
Host‐specific compatibility between RNF138‐like proteins and flavivirus NS5 determines NS5 stability. Mammalian RNF138 but not arthropod homologs recognizes and induces conserved NS5/RdRp K48‐linked ubiquitination and proteasomal degradation, thereby restricting viral replication. Ectopic RNF138 in mice attenuates TBEV‐induced pathogenesis. (Created in
Jialiang Sun, Weijing Yang, Hao Zhou, Shuai Li, Xin Jiang, Jianyang Gu, Wenyan Zhang   +6 more
wiley   +1 more source

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +1 more source

Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum [PDF]

Clinical Microbiology and Infection, 2007
Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 ...
Cordeiro, R. de Aguiar, Nogueira Brilhante, R. S., Gadelha Rocha, M. F., Araujo Moura, F. E., Camargo, Zoilo Pires de UNIFESP, Costa Sidrim, J. J.   +5 more
openaire   +3 more sources

Designer Dynamic DNA Nanoaggregate in Living Cell for Mitochondrial Energy Restriction

Advanced Science, EarlyView.
This study presents the Tech‐tetrahedron, a designer dynamic DNA nanoaggregate engineered for precise mitochondrial energy restriction. Its trinity‐functionalized design integrates navigable unit, telomerase‐activated latch, and self‐assembly module.
Ruijia Deng, Jing Sheng, Ben Niu, Wenjuan Fu, Yingjie Yang, Zuowei Xie, Shuang Xie, Yunxuan He, Meilin Gong, Jue Wang, Yan Pi, Ming Chen, Kai Chang   +12 more
wiley   +1 more source