Results 51 to 60 of about 97,228 (263)

Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum [PDF]

Clinical Microbiology and Infection, 2007
Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 ...
Cordeiro, R. de Aguiar, Nogueira Brilhante, R. S., Gadelha Rocha, M. F., Araujo Moura, F. E., Camargo, Zoilo Pires de UNIFESP, Costa Sidrim, J. J.   +5 more
openaire   +3 more sources

HNRNPD Induces Radioresistance in Nasopharyngeal Carcinoma by Sequestering GRAMD4 mRNA in Stress Granules

Advanced Science, EarlyView.
HNRNPD promotes radioresistance in nasopharyngeal carcinoma by enhancing stress granule assembly and sequestering GRAMD4 mRNA. This suppresses GRAMD4 translation and inhibits mitochondrial apoptosis. Targeting the integrated stress response with ISRIB restores GRAMD4 expression and sensitizes tumors to radiotherapy, revealing a translational control ...
Yingzi Li, Tong Xiang, Yuanyuan Liu, Desheng Weng, Jingjing Zhao, Hao Chen, Yan Tang, Songzuo Xie, Hao Zhang, Jun Luo, Xinyi Yang, Qiuzhong Pan, Muping Di, Jianchuan Xia   +13 more
wiley   +1 more source

Evaluation of Group specific Nested PCR for detection of Bluetongue virus [PDF]

Veterinary World, 2009
During the present study RT-PCR and nested PCR was evaluated for the detection of BTV in blood samples collected from suspected cases of bluetongue. Due to conserved nature, NS1 gene was targeted for the development of partial length and nested PCR assay.
Chauhan H C, Kher H N, Chandel B S, Dadawala A I, Jain L.,Agrawal S M and Bhadaniya A   +4 more
doaj  

Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients Detecção de herpesvirus humano-7 por nested-PCR qualitativo: comparação entre indivíduos sadios e receptores de transplante hepático

Revista da Sociedade Brasileira de Medicina Tropical, 2008
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host.
Ronaldo Luis Thomasini, Juliana de Moraes Martins, Daniela Corte Parola, Sandra Helena Alves Bonon, Ilka de Fátima Santana Ferreira Boin, Luis Sérgio Leonardi, Marília Leonardi, Sandra Cecília Botelho Costa   +7 more
doaj   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

Rapid Multiplex Nested PCR for Detection of Respiratory Viruses [PDF]

Journal of Clinical Microbiology, 2007
ABSTRACT Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 ...
W Y, Lam, Apple C M, Yeung, Julian W, Tang, Margaret, Ip, Edward W C, Chan, Mamie, Hui, Paul K S, Chan   +6 more
openaire   +2 more sources

Haplotype‐Resolved 3D Genomic Landscapes and Their Impacts on Agronomic Traits in Grapevine

Advanced Science, EarlyView.
This study presents a haplotype‐resolved 3D genomic landscape of grapevine, revealing that structural variations (SVs) are closely associated with phased topologically associating domain (TAD) boundary transitions. These rearrangements coordinate with allele‐specific DNA methylation (ASM) and allele‐biased gene expression (ASE) to shape key agronomic ...
Yanling Peng, Lianzhu Zhou, Xinyue Fang, Ruoyan Zhao, Qi Xu, Mengrui Du, Ting Hou, Guizhou Huang, Yingchun Zhang, Sheng Yan, Ruo Yang, Zhongxin Jin, Yang Dong, Yanshuai Xu, Hua Xiao, Xiaodong Xu, Yi Liao, Xiping Wang, Yongfeng Zhou   +18 more
wiley   +1 more source

SAA/FPR2 Signaling Between Pericentral Hepatocytes and Macrophages Exacerbates Zonated Liver Transplant Injury

Advanced Science, EarlyView.
After liver transplantation, ischemia‐reperfusion injury is more severe in pericentral regions. Multiomic analyses of human grafts and mouse models identify FOXO1 activation in pericentral hepatocytes as an upstream driver of SAA secretion. SAA recruits and activates FPR2+ macrophages, amplifying local inflammation. Amilo‐5MER inhibits SAA bioactivity,
Feng Zhang, Rong Li, Tingting Wang, Jianhao Zhang, Zhengqi Wu, Qiang You, Xuying Liu, Cuicui Xiao, Jiebin Zhang, Haitian Chen, Jiaqi Xiao, Jia Yao, Jun Zheng, Yingcai Zhang, Hua Li, Shuhong Yi, Yang Yang, Qi Zhang, Xiaofeng Yuan, Yasong Liu   +19 more
wiley   +1 more source

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +1 more source

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source