Results 51 to 60 of about 1,037,260 (308)

Primer pairs used for boost/nested PCR.

, 2013
Primer pairs used for boost/nested PCR.
Cristianne M. M. Cordeiro (504656), Hamed Esmaili (504657), George Ansah (504658), Maxwell T. Hincke (504659)   +3 more
core   +1 more source

Canonical Antibodies Adopt Distinct Binding Modes to Recognize Viral Glycan Shields

Advanced Science, EarlyView.
Canonical Y‐shaped antibodies recognize viral glycan shields through adaptive Fab assembly states shaped by glycan organization and somatic hypermutation. Structural analyses ofbroadly neutralizing antibodies VRC35 and VRC36 across glycoproteins of HIV‐1, influenza, SARS‐CoV‐2, and Lassa viruses reveal distinct Fab assembly states, spanning monovalent,
Jiaxuan Cheng, Evan M. Cale, Amirabbas Maghsoudi, Matthew S. Sutton, Nancy S. Longo, Rebecca A. Gillespie, Lingshu Wang, Ivan Kosik, Sue Chong, Atsuhiro Yasuhara, Tatsiana Bylund, Arne Schön, Prabhanshu Tripathi, Yaroslav Tsybovsky, Haotian Lei, Nicholas C. Morano, Allison B. Lupatkin, Abraham J. Morton, Zabrina C. Lang, Jordan E. Becker, Isabella R. Frascilla, Myungjin Lee, Ning Li, Cuiping Liu, Ryan S. Roark, Chen‐Hsiang Shen, I‐Ting Teng, David J. Van Wazer, Danyi Wang, Lingyuan Wu, Goran Ahlsen, Mike Castro, Haijuan Du, Michael J. Ernandes, Tiansheng Li, Bob C. Lin, Mark K. Louder, Krisha McKee, Jonah S. Merriam, Sijy O'Dell, Li Ou, Sergei Pletnev, David Prikryl, Qi Qiu, Sarah Rubin, Mallika Sastry, Stephen D. Schmidt, Asif Shajahan, Andrea R. Shiakolas, Sanjay Srivatsan, Baoshan Zhang, Qiong Zhou, Mark Connors, Jason G. Gall, Yicheng Guo, Rick K. Huang, Yaoxing Huang, Richard A. Koup, Q. Paula Lei, John R. Mascola, Reda Rawi, Leonid Serebryannyy, Lawrence Shapiro, Zizhang Sheng, David D. Ho, Patrick C. Wilson, Jonathan W. Yewdell, Theodore C. Pierson, Masaru Kanekiyo, Nicole A. Doria‐Rose, Peter D. Kwong, Tongqing Zhou   +71 more
wiley   +1 more source

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Nested PCR espécie-específica para o diagnóstico da infecção por Brucella ovis em carneiros

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj  

Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

Revista Brasileira de Parasitologia Veterinária, 2018
This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo, Bruna Nicoleti Santana, Elis Domingos Ferrari, Alex Akira Nakamura, Walter Bertequini Nagata, Ana Rita Moraes Nardi, Marcelo Vasconcelos Meireles   +6 more
doaj   +1 more source

Evaluation of Pan-Dermatophyte Nested PCR in Diagnosis of Onychomycosis [PDF]

Journal of Clinical Microbiology, 2007
ABSTRACT In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene ( CHS1 ) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis.
Jaya, Garg, Ragini, Tilak, Sanjay, Singh, Anil Kumar, Gulati, Atul, Garg, Pradyot, Prakash, Gopal, Nath   +6 more
openaire   +2 more sources

Primers used for nested RT-PCR.

, 2012
Primers used for nested RT-PCR.
Jun Yin (94374), Jingjing Mao (175629), Ruiwen Li (175622), Peng Xiao (172430), Wengui Li (175626), Quan Sun (175631), Ruiping She (175623)   +6 more
core   +1 more source

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

BMC Infectious Diseases, 2005
Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M, Volckaert Guido, Messmer Trudy O, Verminnen Kristel, Van Loock Marnix, Vanrompay Daisy   +5 more
doaj   +1 more source

Detection of cytomegalovirus using ‘boosted’ nested PCR

Molecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg, S A, Nordbø, P, Winge, A, Dalen   +3 more
openaire   +2 more sources

Detecção por PCR de milho geneticamente modificado (MON810) em farinha de milho, fubá, biju e polenta [PDF]

, 2007
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-graduação em Ciência dos AlimentosNeste trabalho, o método da Reação em Cadeia da Polimerase (PCR) foi utilizado para detecção do milho ...
Dinon, Andréia Zilio
core