Results 71 to 80 of about 1,037,260 (308)

Rapid Multiplex Nested PCR for Detection of Respiratory Viruses [PDF]

Journal of Clinical Microbiology, 2007
ABSTRACT Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 ...
W Y, Lam, Apple C M, Yeung, Julian W, Tang, Margaret, Ip, Edward W C, Chan, Mamie, Hui, Paul K S, Chan   +6 more
openaire   +2 more sources

Standard nested-PCR mastermix reagent volumes.

, 2014
Standard nested-PCR mastermix reagent volumes.
Stephen A. Clark (526800), Ray Borrow (49342), Lynne S. Newbold (526801), Jay Lucidarme (366721)   +3 more
core   +1 more source

Transient Sleep Deprivation Induces Persistent Auditory Neuropathy via ROS‐Initiated Neuroinflammation and BK Channel Suppression

Advanced Science, EarlyView.
Sleep disturbance severity closely tracks hearing loss in a clinical cohort, yet the mechanistic link remains unclear. Acute sleep deprivation is shown to trigger transient cochlear oxidative stress that switches into a self‐sustaining neuroinflammatory state, suppressing BK channels and causing irreversible synaptopathy.
Dan Chen, Ying Ma, Lixuan Huang, Xin Chen, Fanliang Kong, Zhenzhou Qian, Mingyue Guan, Xinlin Wang, Yanyan Yang, Shasha Zhang, Peng Li, Renjie Chai   +11 more
wiley   +1 more source

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +1 more source

Nested PCR in lung tissue for diagnosis of pulmonary tuberculosis [PDF]

European Respiratory Journal, 2009
Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB.Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies ...
J S, Park, Y A, Kang, S Y, Kwon, H I, Yoon, J-H, Chung, C-T, Lee, J H, Lee   +6 more
openaire   +3 more sources

Phase Separation of SF3B1 Serves as a Critical Post‐Transcriptional Regulator During Early Mouse Embryogenesis

Advanced Science, EarlyView.
Phase separation of SF3B1 acts as a key regulatory mechanism for dynamic alternative splicing throughout early mouse embryogenesis. Its absence triggers extensive splicing errors, which induce persistent DNA damage, defective cell cycle progression, and failed cell lineage commitment, and ultimately hinder the normal growth and development of ...
Kang Zhao, Ting‐Yu Han, Yan‐Li Cheng, Yi‐Dan Zhang, Yu‐Wei Zhang, JinJian Guo, ShaoJun Zhang, WenZe Huang, Jing Zhang, Pei‐Yu Liao, Ying Xin, ChuanChen Chu, Qing‐Yuan Sun, ZhiZhen Liu, Xiang‐Hong Ou, Jun Xie   +15 more
wiley   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

Primer and probe sequences used for nested reverse transcriptase (RT)-PCR and real-time RT-PCR.

, 2016
Primer and probe sequences used for nested reverse transcriptase (RT)-PCR and real-time RT-PCR.
Yuko Sato (111419), Fumihiro Taguchi (2142745), Kunihisa Kozawa (296839), Tadaki Suzuki (251922), Asif Naeem (2142742), Noriko Nakajima (249982), Hideki Hasegawa (249991), Noriyo Nagata (389752), Osamu Kotani (771314), Takushi Hosomi (106183), Naoko Iwata-Yoshikawa (325679), Hiroyuki Shimizu (201492), Hiroyuki Tsukagoshi (2142748)   +12 more
core   +1 more source

Sensitivity test of the nested PCR assay.

, 2022
Ten-fold dilutions (1 ng to 10 fg) of C. siamense ML133 genomic DNA were used as the template. The nested PCR assay was performed using primers Col_nest-1F/Col_nest-1R for the first PCR, and Col_nest-2F/Col_nest-2R for the second PCR.
Chia-Lin Chung (815923), Ting-Hsuan Hung (304382), Hung-Yi Wu (409276), Yi-Chia Chen (5677163), Pei-Che Chung (12969231)   +4 more
core   +1 more source

TNAP and PHOSPHO1 Function Synergistically to Afford Critical Control Over the Mineralization of the Postnatal Murine Skeleton

Advanced Science, EarlyView.
Biomineralization underpins skeletal development, yet its molecular control remains incompletely understood. Using a novel murine knockout model, this study reveals the essential and complementary roles of PHOSPHO1 and TNAP in postnatal skeletal development.
Lucie E. Bourne, Aikta Sharma, Scott Dillon, Jacob Keen, Soher N. Jayash, Natalie Crump, Lucinda A.E. Evans, Maya Karmali, Worachet Promruk, Claire E. Clarkin, Sonoko Narisawa, Louise Stephen, Brian L. Foster, José Luis Millán, Colin Farquharson, Katherine A. Staines   +15 more
wiley   +1 more source