Results 71 to 80 of about 986,696 (337)

Cerium Nanoparticle‐Mediated Inhibition of the NSUN2/m5C Axis Suppresses Synovial Aggression in Rheumatoid Arthritis

Advanced Science, EarlyView.
A novel epitranscriptomic mechanism in rheumatoid arthritis is uncovered: NSUN2 promotes disease via m5C‐dependent stabilization of ICMT mRNA, fueling the migration and invasion of pathogenic RA FLS. Targeting this axis with engineered nanoparticles (Ce/SAA NPs) effectively inhibits disease progression, presenting a precise therapeutic strategy ...
Ruiru Li, Simin Chen, Huijuan Hu, Shiyao Wu, Jingnan Wang, Kai Sun, Chenxi Peng, Suling Liu, Shuoyang Zhang, Yu Kuang, Wei Chen, Qian Qiu, Liuqin Liang, Yu Wen, Hanshi Xu, Youjun Xiao   +15 more
wiley   +1 more source

Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients Detecção de herpesvirus humano-7 por nested-PCR qualitativo: comparação entre indivíduos sadios e receptores de transplante hepático

Revista da Sociedade Brasileira de Medicina Tropical, 2008
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host.
Ronaldo Luis Thomasini, Juliana de Moraes Martins, Daniela Corte Parola, Sandra Helena Alves Bonon, Ilka de Fátima Santana Ferreira Boin, Luis Sérgio Leonardi, Marília Leonardi, Sandra Cecília Botelho Costa   +7 more
doaj   +1 more source

Detection of Candida albicans by Nested PCR

Journal of the Japanese Association for Infectious Diseases, 1994
A nested PCR assay was developed for the diagnosis of Candida albicans infection. A segment of the C. albicans beta-tubulin gene was amplified in this assay that was sensitive with the detection limit of 10 fg DNA of C. albicans. This assay was found to be specific by obtaining the negative results with 13 organisms (5 other Candida, 2 Cryptococcus, 3 ...
openaire   +3 more sources

Correction to: UPrimer: A Clade-Specific Primer Design Program Based on Nested-PCR Strategy and Its Applications in Amplicon Capture Phylogenomics. [PDF]

Mol Biol Evol
europepmc   +3 more sources

Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces [PDF]

, 2003
A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and
Phillips, N.D., La, T., Hampson, D.J.
core  

Introducing Borsantrazole: A Trifunctional Boron‐Based Pyrazole That Extends the Lifespan of Amyotrophic Lateral Sclerosis Mice

Advanced Science, EarlyView.
Herein we report a boron‐based pyrazole, (Borsantrazole ‐ a small molecule that selectively targets oxidative stress) that significantly increases survival, reduces weight loss, delays disease onset, and affects global protein changes in the SOD1‐G37R mouse model of ALS.
Nitesh Sanghai, Rhonda Kelley, Ying Lao, M. Immanuel Reyes Madlangsakay, Prasanta Paul, M. Alejandra Llanes‐Cuesta, Jun‐Feng Wang, René P. Zahedi, Jiming Kong, Geoffrey K. Tranmer   +9 more
wiley   +1 more source

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source

Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum [PDF]

Clinical Microbiology and Infection, 2007
Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 ...
Cordeiro, R. de Aguiar, Nogueira Brilhante, R. S., Gadelha Rocha, M. F., Araujo Moura, F. E., Camargo, Zoilo Pires de UNIFESP, Costa Sidrim, J. J.   +5 more
openaire   +3 more sources

Neuron‐Targeted Exosomal Delivery of siRNA Against RIPK3 Slows Neurodegenerative Progression in Alzheimer's Disease

Advanced Science, EarlyView.
Engineered exosomal siRNA delivery platform enables systemic, neuron‐targeted RNA transport across the blood–brain barrier. Surface functionalization with rabies virus glycoprotein‐derived peptide facilitates receptor‐mediated transcytosis, achieving efficient cytosolic delivery and robust gene silencing. Targeting RIPK3 suppresses necroptosis, reduces
Chi Zhang, Jiaqi Zhang, Yuzhi Wang, Yaodong Wang, Peng Lin, Yanling Wang, Zixuan Tang, Jintai Yu, Qin Zhou, Feiyun Cui   +9 more
wiley   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source