Results 71 to 80 of about 97,549 (263)

Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge

Archives of Environmental Protection, 2014
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra, Wiszniowski Jarosław, Ciesielski Sławomir   +2 more
doaj   +1 more source

Neuron‐Targeted Exosomal Delivery of siRNA Against RIPK3 Slows Neurodegenerative Progression in Alzheimer's Disease

Advanced Science, EarlyView.
Engineered exosomal siRNA delivery platform enables systemic, neuron‐targeted RNA transport across the blood–brain barrier. Surface functionalization with rabies virus glycoprotein‐derived peptide facilitates receptor‐mediated transcytosis, achieving efficient cytosolic delivery and robust gene silencing. Targeting RIPK3 suppresses necroptosis, reduces
Chi Zhang, Jiaqi Zhang, Yuzhi Wang, Yaodong Wang, Peng Lin, Yanling Wang, Zixuan Tang, Jintai Yu, Qin Zhou, Feiyun Cui   +9 more
wiley   +1 more source

Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical, 2007
A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR) foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do ...
Karla Valéria Batista Lima, Maria Luíza Lopes, Edvaldo Carlos Brito Loureiro, Maurimélia Mesquita Costa, Ninarosa Calzavara Cardoso, George Leandro Ferreira Lima, Maísa Silva Sousa   +6 more
doaj   +1 more source

HD‐tDCS Restores Perivascular AQP4 Polarization via PPARγ Modulation to Enhance Glymphatic Clearance After Intracerebral Hemorrhage in Mice

Advanced Science, EarlyView.
HD‐tDCS promotes recovery after intracerebral hemorrhage by enhancing PPARγ‐dependent astrocyte remodeling in a mouse model of ICH. This process suppresses proinflammatory astrocytic activation, restores perivascular AQP4 repolarization, and improves glymphatic influx and efflux, thereby facilitating hematoma and perihematomal edema clearance and ...
Zhiming Li, Yingmei Zhang, Qianqian Tong, Zhitao Gong, Shanyu Zhang, Zhen Qian, Hui Zhang, Qian Yao, Qi Li   +8 more
wiley   +1 more source

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquímica e nested-PCR

Revista Brasileira de Parasitologia Veterinária, 2009
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB).
Aline Diniz Cabral, Clarice Neves Camargo, Nara Thiers Cacciatori Galleti, Liria Hiromi Okuda, Edviges Maristela Pituco, Claudia Del Fava   +5 more
doaj   +1 more source

Refining a preclinical model of viral myocarditis in accordance with biotech standards

Animal Models and Experimental Medicine, EarlyView.
This study aimed to refine a murine model of Coxsackievirus B3‐induced myocarditis by integrating dietary and imaging innovations to improve animal welfare, data quality, and clinical applicability. The refined diet significantly reduced animal health burden, reduced weight loss, and stabilized blood glucose during development of cardiac inflammation ...
Jonas Stewen, Julia Riedesel, Juliane Stein, Brandon H. Greene, Svenja Rohde, Sonja Suess, Bjoern Keiner, Priya Panjwani, Padmapriya Ponnuswamy, Juan E. Camacho Londoño   +9 more
wiley   +1 more source

Enabling the study of gene function in gymnosperms: Virus‐induced gene silencing in Ephedra tweedieana

Applications in Plant Sciences, EarlyView.
Abstract Premise As the sister clade to angiosperms, extant gymnosperms are crucial for reconstructing ancestral gene regulatory networks in seed plants. This highlights the need for model systems representing each of their distinct lineages. However, tools to quickly and effectively investigate gene function in gymnosperms are still limited due to the
Anthony G. K. Garcia, Jo Trang Bùi, Todd P. Michael, Stefanie M. Ickert‐Bond, Verónica S. Di Stilio   +4 more
wiley   +1 more source

Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Universa Medicina, 2007
Latar Belakang DNA rpob (RNA polymerase sub unit b) Mycobacterium tuberculosis dapat diamplifikasi secara spesifik dengan metode nested polymerase chain reaction (PCR).
Maria Lina Rosilawati
doaj   +1 more source

MicroCT reinvestigation of the only articulated fossil anostomid fish reveals synonymy of Arhinolemur Ameghino, 1898 and Megaleporinus Ramirez et al., 2017

The Anatomical Record, EarlyView.
Abstract Arhinolemur scalabrinii† Ameghino, 1898 was originally described as a strepsirrhine primate (Mammalia) but has been recognized as an anostomid fish since 2012. It remains the only extinct anostomid species known from complete cranial material.
Karen M. Panzeri, Humberto L. Andrelino, Sergio Bogan, José P. O'Gorman, Federico Agnolín, Brian L. Sidlauskas, Nahuel Vega, Agustín G. Martinelli, José L. Birindelli   +8 more
wiley   +1 more source

Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome

Memorias do Instituto Oswaldo Cruz, 2004
We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS ...
Marcos Lázaro Moreli, Ricardo Luiz Moro de Sousa, Luiz Tadeu Moraes Figueiredo   +2 more
doaj   +1 more source