Results 81 to 90 of about 1,037,260 (308)

Nested RT-PCR for ante mortem diagnosis of rabies from body secretion/excretion of animals suspected for rabies

Veterinary World, 2012
Aim: The present study deals with molecular technique Nested RT-PCR for detection of rabies viral RNA from biological fluid samples (Saliva, Milk and Urine) collected from animal suspected for rabies and to compare the sensitivity of Nested RT-PCR ...
M Dandale, C K Singh, V Ramneek, D Deka, B S Sandhu, K Bansal, N K Sood   +6 more
doaj   +1 more source

Electrophoretic comparisons of PCR products from conventional and nested PCR and dilution series analyses.

, 2019
A- conventional PCR of five different samples; B-nested PCR of the same five samples as shown in A; C-conventional PCR of 105 fold dilution series of PCR product shown in Fig 2A-lane 5 and D-nested PCR of dilution series of PCR product shown in Fig 2-B ...
Yongyang Luo (6393332), Yanyun Hong (6393329), Jianglan Yi (6393335), Tuyong Yi (6393338), Ling He (282204), Liangying Dai (713014)   +5 more
core   +1 more source

Mapping Embryonic Mouse Lung Development Using Enhanced Spatial Transcriptomics

Advanced Science, EarlyView.
The mammalian lung contains ∼50 cell types arranged along the airway epithelium. Here, deterministic barcoding sequencing (DBiT‐seq) is optimized to nearly double transcript recovery, enabling highly sensitive spatial mapping of the embryonic mouse lung.
Pengfei Zhang, Benjamin K. Law, Niles Huang, Katharine Goodwin, Celeste M. Nelson, Michelle M. Chan   +5 more
wiley   +1 more source

Performance of nested-RT-PCR assays for Zika and Dengue detection

Acta Scientiarum: Biological Sciences
Arboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses.
José Henrique Francisco Roma, Rachel Cruz Alves, Stefhano Luis Candido, Valéria Dutra, Luciano Nakazato, Renata Dezengrini-Slhessarenko, Juliana Helena Chavez-Pavoni, Mariângela Ribeiro Resende   +7 more
doaj   +1 more source

Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

Malaria Journal, 2012
Background A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes.
Lee Pei-Wen, Ji Dar-Der, Liu Chia-Tai, Rampao Herodes S, do Rosario Virgilio E, Lin I-Feng, Shaio Men-Fang   +6 more
doaj   +1 more source

RT-PCR/Nested conditions for amplification for rotavirus and picobirnavirus.

, 2021
RT-PCR/Nested conditions for amplification for rotavirus and picobirnavirus.
Helder Henrique Costa Pinheiro (6095834), Elaine Hellen Nunes Chagas (11418812), Cinthia Távora de Albuquerque Lopes (11418827), Felipe Masiero Salvarani (11418833), Thaymis Kiara Santos Sousa (11418824), Ana Carolina Silva Serra (11197230), Hanna Gabriela da Silva Oliveira (11418821), Yashpal Singh Malik (3156804), Joana D’Arc Pereira Mascarenhas (11418836), Edvaldo Tavares da Penha Júnior (11418818), Ricardo José de Paula Souza e Guimarães (10521550), Lizandra Caroline dos Santos Souto (11418815), Renato da Silva Bandeira (4102642), Sheyla Farhayldes Souza Domingues (11418830), José Wandilson Barboza Duarte Júnior (11418809)   +14 more
core   +1 more source

Raman Analysis of RNA Nucleotide Strands From SARS‐CoV‐2 Variants and Subvariants: A Step Forward in the Definition of “Raman Genome”

Advanced Science, EarlyView.
Main Raman signals in RNA, related to helical backbone, ribofuranose ring, and nitrogenous bases, are affected by secondary structures that cause hyperchromic effects and significantly alter the Raman intensity. ABSTRACT Raman spectra of RNA nucleotide strands extracted from 20 SARS‐CoV‐2 strains, including the original Japanese isolate, Alpha, Beta ...
Giuseppe Pezzotti, Yoshiki Yasukochi, Gianluca Angiola, Takaharu Ueno, Saki Ikegami, Rintaro Okawa, Tetsuya Adachi, Wenliang Zhu, Osam Mazda, Alfio Grillo, Koichiro Higasa, Kazu Okuma   +11 more
wiley   +1 more source

PCR primers used for nested PCR.

, 2015
Primers’ genome location: F1-14 492; R1-14 828; F2-14 528; R2-14 721; F3-1 642 737; R3-1 643 023; F4-1 642 764; R4-1 642 925; F5-2 935 537; R5-2 935 804; F6-2 935 594; R6-2 935 716PCR primers used for nested PCR.
Florence Brossier (803925), Emmanuelle Cambau (206542), Aurélie Chauffour (485000), Vincent Jarlier (243571), Alexandra Aubry (243572), Florence Reibel (636374)   +5 more
core   +1 more source

SOX30 Facilitates Triple‐Negative Breast Cancer Metastasis via TNFR2–NF‐κB Signaling and Tumor Microenvironment Remodeling

Advanced Science, EarlyView.
SOX30 drives triple‐negative breast cancer (TNBC) metastasis through a dual mechanism: direct activation of the TNFR2/NF‐κB signaling cascade and subsequent CCL20‐mediated recruitment of tumor‐associated macrophages (TAMs) into the tumor microenvironment.
Pingping Gao, Na Sun, Ning Yang, Tingting Zhao, Rui Fu, Zhenyu Wu, Yuqin Zhou, Xuanni Tan, Yi Zhang, Fei Han, Xiaowei Qi   +10 more
wiley   +1 more source

Aplicação da Técnica de “NESTED PCR” Durante o Período Pré-Patente para Identificação de Schistosoma Mansoni no Hospedeiro Intermediário Biomphalaria Glabrata [PDF]

, 2010
Biomphalaria glabrata, molusco de água doce, desempenha um importante papel em Parasitologia Médica, por ser o hospedeiro intermediário de Schistosoma mansoni, tremátode digenético responsável pela schistosomose intestinal.
Leal, Silvânia da Veiga
core