Results 61 to 70 of about 986,696 (337)

Increased sensitivity for detecting malaria parasites in human umbilical cord blood using scaled-up DNA preparation. [PDF]

open access: yes, 2012
BACKGROUND: All mothers donating umbilical cord blood units to the NHS cord blood bank undergo an assessment for the likelihood of prior exposure to malaria infection.
Fiona Regan   +14 more
core   +1 more source

FastNano Liquid: An Automated Platform for Small‐Angle X‐ray Scattering‐Based Materials Discovery

open access: yesAdvanced Engineering Materials, EarlyView.
We present FastNano Liquid, an automated small‐ and wide‐angle X‐ray scattering platform for the combined synthesis and characterization of (nano)materials. The platform is coupled to varied reactor workflows for both in situ studies of reaction kinetics and ex situ screening of synthesis conditions to support machine learning‐guided exploration ...
Pierre‐Baptiste Flandrin   +16 more
wiley   +1 more source

DNA methods for the detection of Phytophthora cinnamomi from soil [PDF]

open access: yes, 2006
This project assesses two aspects of DNA detection of Phytophthora species from soil samples. Firstly, a nested PCR protocol was established with both primary and nested PCR specific for P. cinnamomi detection. PCR amplification of P.
Anderson, Nari Michelle
core  

Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

open access: yesRevista Brasileira de Parasitologia Veterinária, 2018
This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo   +6 more
doaj   +1 more source

A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels

open access: yesFrontiers in Microbiology, 2022
Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii.
Susana Ruiz-Ruiz   +18 more
doaj   +1 more source

Evaluation of Pan-Dermatophyte Nested PCR in Diagnosis of Onychomycosis [PDF]

open access: yesJournal of Clinical Microbiology, 2007
ABSTRACT In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene ( CHS1 ) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis.
Jaya, Garg   +6 more
openaire   +2 more sources

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

open access: yesAdvanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang   +11 more
wiley   +1 more source

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

open access: yesBMC Infectious Diseases, 2005
Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M   +5 more
doaj   +1 more source

Detection of cytomegalovirus using ‘boosted’ nested PCR

open access: yesMolecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg   +3 more
openaire   +2 more sources

Development of B1 nested PCR for assessing the prevalence of zoonotic protozoan disease agent toxoplasma gondii among food animals from Karnataka state, Southern India [PDF]

open access: yes, 2018
Toxoplasmosis samples meat or portions edible from gondii Toxoplasma of infection natural of detection direct for aimed was study present The. birds intended stalls fish and mutton available locally the from chicken backyard and fishes, goats and sheep ...
Rajendran, C.   +4 more
core  

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