Results 61 to 70 of about 97,549 (263)

Rapid Multiplex Nested PCR for Detection of Respiratory Viruses [PDF]

Journal of Clinical Microbiology, 2007
ABSTRACT Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 ...
W Y, Lam, Apple C M, Yeung, Julian W, Tang, Margaret, Ip, Edward W C, Chan, Mamie, Hui, Paul K S, Chan   +6 more
openaire   +2 more sources

Placental Site Trophoblastic Tumor Acquires Immune Functions by Incorporating Host Maternal Genes

Advanced Science, EarlyView.
PSTT cells, through cell fusion with B cells, incorporate abundant non‐inherited maternal genes that are detectable by DNIMA. These hybrid cells acquire immunotherapy‐resistant genetic changes and increase the expression of B cell‐derived immune‐related molecules such as Ig, HLA, LILRB, SIGLEC10, and so on, creating an immunotolerant environment around
Kyosuke Kagami, Masanori Ono, Yasunari Mizumoto, Tatsuhito Kanda, Takashi Iizuka, Takiko Daikoku, Shin‐ichi Horike, Akira Hattori, Akihito Horie, Sachiko Minamiguchi, Tomoko Fujiwara, Kazuyoshi Hosomichi, Atsushi Tajima, Hirokazu Usui, Kaoru Abiko, Hiroshi Fujiwara   +15 more
wiley   +1 more source

Nested RT-PCR for ante mortem diagnosis of rabies from body secretion/excretion of animals suspected for rabies

Veterinary World, 2012
Aim: The present study deals with molecular technique Nested RT-PCR for detection of rabies viral RNA from biological fluid samples (Saliva, Milk and Urine) collected from animal suspected for rabies and to compare the sensitivity of Nested RT-PCR ...
M Dandale, C K Singh, V Ramneek, D Deka, B S Sandhu, K Bansal, N K Sood   +6 more
doaj   +1 more source

HLA‐DR+ Schwann Cells Generate the Protumor Cancer‐Neuron‐Immune Niche in Head and Neck Squamous Cell Carcinoma

Advanced Science, EarlyView.
Hijacked and educated by HNSCC cells, HLA‐DR+ Schwann cells lost their normal neural‐related functions but acquired immunoregulatory phenotypes to promote CD4+ T cells transform into Tregs. HLA‐DR+ Schwann cells induced a macrophage subpopulation, Il1β.
Xiaoyan Meng, Zhonglong Liu, Shijian Zhang, Luoman Gan, Liren Cao, Jingjing Sun, Lingfang Zhang, Yue He   +7 more
wiley   +1 more source

Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé

Malaria Journal, 2012
Background A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes.
Lee Pei-Wen, Ji Dar-Der, Liu Chia-Tai, Rampao Herodes S, do Rosario Virgilio E, Lin I-Feng, Shaio Men-Fang   +6 more
doaj   +1 more source

Nested PCR for detection of mutans streptococci in dental plaque [PDF]

Letters in Applied Microbiology, 2003
Mutans streptococci such as Streptococcus mutans and Streptococcus sobrinus have been implicated in human dental caries. In an attempt to develop a rapid and sensitive method for detecting Strep. mutans and Strep. sobrinus in dental plaque, a nested PCR amplification based on the 16S rRNA gene was employed.A universal set of PCR primers for bacterial ...
T, Sato, J, Matsuyama, T, Kumagai, G, Mayanagi, M, Yamaura, J, Washio, N, Takahashi   +6 more
openaire   +2 more sources

Stiffness‐Activated Stellate Cells Drive Pancreatic Cancer Liver Colonization via GMFG‐TNS4 Signaling

Advanced Science, EarlyView.
Fibrotic liver stiffness activates hepatic stellate cells through Piezo1‐dependent calcium influx and ER stress, promoting EV‐associated GMFG release. Delivered GMFG engages TNS4 in pancreatic cancer cells, triggering FAK/AKT signaling, adhesion, and fatty acid synthesis.
Biwen Zhu, Jian Wan, Xue Zhang, Jiashuai Yan, Xi Chen, Xiaoqi Guan, Qianqian Ren, Tao Yang, Di Wu, Qingsong Guo, Yibing Guo, Yuhua Lu   +11 more
wiley   +1 more source

Performance of nested-RT-PCR assays for Zika and Dengue detection

Acta Scientiarum: Biological Sciences
Arboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses.
José Henrique Francisco Roma, Rachel Cruz Alves, Stefhano Luis Candido, Valéria Dutra, Luciano Nakazato, Renata Dezengrini-Slhessarenko, Juliana Helena Chavez-Pavoni, Mariângela Ribeiro Resende   +7 more
doaj   +1 more source

Cerium Nanoparticle‐Mediated Inhibition of the NSUN2/m5C Axis Suppresses Synovial Aggression in Rheumatoid Arthritis

Advanced Science, EarlyView.
A novel epitranscriptomic mechanism in rheumatoid arthritis is uncovered: NSUN2 promotes disease via m5C‐dependent stabilization of ICMT mRNA, fueling the migration and invasion of pathogenic RA FLS. Targeting this axis with engineered nanoparticles (Ce/SAA NPs) effectively inhibits disease progression, presenting a precise therapeutic strategy ...
Ruiru Li, Simin Chen, Huijuan Hu, Shiyao Wu, Jingnan Wang, Kai Sun, Chenxi Peng, Suling Liu, Shuoyang Zhang, Yu Kuang, Wei Chen, Qian Qiu, Liuqin Liang, Yu Wen, Hanshi Xu, Youjun Xiao   +15 more
wiley   +1 more source

Introducing Borsantrazole: A Trifunctional Boron‐Based Pyrazole That Extends the Lifespan of Amyotrophic Lateral Sclerosis Mice

Advanced Science, EarlyView.
Herein we report a boron‐based pyrazole, (Borsantrazole ‐ a small molecule that selectively targets oxidative stress) that significantly increases survival, reduces weight loss, delays disease onset, and affects global protein changes in the SOD1‐G37R mouse model of ALS.
Nitesh Sanghai, Rhonda Kelley, Ying Lao, M. Immanuel Reyes Madlangsakay, Prasanta Paul, M. Alejandra Llanes‐Cuesta, Jun‐Feng Wang, René P. Zahedi, Jiming Kong, Geoffrey K. Tranmer   +9 more
wiley   +1 more source