Results 31 to 40 of about 97,450 (165)
Nested real-time PCR for hepatitis A detection [PDF]
Letters in Applied Microbiology, 2009 The hepatitis A virus (HAV) is one of the most important human foodborne pathogens causing a number of worldwide outbreaks each year. The detection of HAV in food samples remains a complex issue, because commonly used detection tools, such as conventional or even real-time PCR assays, are often unable to detect HAV with sufficient sensitivity. The aims
Y, Hu, I, Arsov
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Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi [PDF]
Pesquisa Veterinária Brasileira, 2011 Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T.
Leal, Danielle C. +4 more
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Research in Molecular Medicine, 2019 Background: Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from
Camellia Taghadosi +4 more
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Pesquisa Veterinária Brasileira, 2009 Pneumocystis has been isolated from a wide range of unrelated mammalian hosts, including humans, domestic and wild animals. It has been demonstrated that the genome of Pneumocystis of one host differs markedly from that of other hosts. Also, variation in
Edna Maria Cavallini Sanches +9 more
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Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013 The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa +8 more
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Revista Brasileira de Parasitologia Veterinária, 2018 This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo +6 more
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A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels
Frontiers in Microbiology, 2022 Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii.
Susana Ruiz-Ruiz +18 more
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Determination of Cryptosporidium spp. in Van cats by nested PCR [PDF]
Medycyna Weterynaryjna, 2018 The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in
Özkan , Cumali +4 more
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BMC Infectious Diseases, 2005 Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M +5 more
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Revista da Sociedade Brasileira de Medicina Tropical, 2008 Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host.
Ronaldo Luis Thomasini +7 more
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