Results 11 to 20 of about 982,945 (306)

A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

Revista da Sociedade Brasileira de Medicina Tropical, 2010
INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi, Débora Fonseca Vituri, Pedro Sebastião Raimundo Dionízio Filho, Alexandre Augusto Sasaki, Maria Sueli Soares Felipe, Emerson José Venancio   +5 more
doaj   +2 more sources

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Nested PCR espécie-específica para o diagnóstico da infecção por Brucella ovis em carneiros

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj   +1 more source

A principled approach to programming with nested types in Haskell [PDF]

, 2009
Initial algebra semantics is one of the cornerstones of the theory of modern functional programming languages. For each inductive data type, it provides a Church encoding for that type, a build combinator which constructs data of that type, a fold ...
Ghani, Neil, Johann, Patricia
core   +4 more sources

Comparative performance of microscopy, nested PCR, and real-time PCR for screening avian haemosporidian parasites in Afrotropical starlings (family Sturnidae)

, 2023
Prevalence studies of avian haemosporidian parasites frequently use microscopy and the nested polymerase chain reaction (PCR) protocols for detecting infections.
Ndlovu, Mduduzi, Wardjomto, Maliki B., Mduduzi Ndlovu, Pori, Tinotendashe, Nangammbi, Tshifhiwa C., Antón Pérez-Rodríguez, Tshifhiwa C. Nangammbi, Tinotendashe Pori, Wardjomto, Maliki, Nangammbi, Tshifhiwa, Maliki B. Wardjomto, Pérez-Rodríguez, Antón   +11 more
core   +1 more source

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller, Ganesh Srinivasamoorthy, Venkatachalam Udhayakumar, John W. Barnwell, Leopoldo Villegas, S. Patrick Kachur, Demas, Allison, Kissinger, Jessica C, Villegas, Leopoldo, Abdunoor M. Kabanywanyi, Jessica C. Kissinger, Deborah Sumari, Escalante, Ananias A, Lucchi, Naomi W, Udhayakumar, Venkatachalam, Sumari, Deborah, Peterson, David S, Ananias A. Escalante, Naomi W. Lucchi, Jeremy DeBarry, DeBarry, Jeremy, David S. Peterson, Oberstaller, Jenna, Allison Demas, Srinivasamoorthy, Ganesh, Kachur, S Patrick, Kabanywanyi, Abdunoor M, Barnwell, John W   +27 more
core   +1 more source

Application of a Nested, Multiplex PCR to Psittacosis Outbreaks [PDF]

Journal of Clinical Microbiology, 1997
We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene.
T O, Messmer, S K, Skelton, J F, Moroney, H, Daugharty, B S, Fields   +4 more
openaire   +3 more sources

Development of Nested-PCR Assay to Detect Acidovorax citrulli, a Causal Agent of Bacterial Fruit Blotch at Cucurbitaceae

Research in Plant Disease, 2015
The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim, Kyoung-Soo Park, Hye-Seong Kim, Hyok-In Lee, Jae-Soon Cha   +4 more
doaj   +1 more source

An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Revista da Sociedade Brasileira de Medicina Tropical, 2013
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fabio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson Jose Venancio   +6 more
doaj   +1 more source

A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand [PDF]

, 2009
Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the
Thompson, R.C.A., K Darwin Murrell, Leelayoova, S, Murrell, K. Darwin, Cribb, Thomas, Cribb, T, Thomas Cribb, Cribb, T., Macaranas, J, Thompson R. C. Andrew, Julie Macaranas, Thompson, RCA, R C Andrew Thompson, Saovanee Leelayoova, Mungthin, M, Macaranas, Julie, Mungthin, M., Leelayoova, Saovanee, Thompson, R. C. Andrew, Macaranas Julie, Murrell K. Darwin, Traub, Rebecca J., Mungthin, Mathirut, Mathirut Mungthin, Traub, RJ, Murrell, KD, Cribb Thomas, Macaranas, J., Traub, R.J., Rebecca J Traub, Leelayoova, S., Traub Rebecca J., Mungthin Mathirut, Murrell, K.D., Leelayoova Saovanee   +34 more
core   +2 more sources

One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples

Frontiers in Veterinary Science, 2020
Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang, Joong Ki Song, Seongho Shin, Ki Myung Choi, Hyunil Kim   +4 more
doaj   +1 more source