Results 11 to 20 of about 188,318 (309)

Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques [PDF]

Scientific Reports
Acanthamoeba keratitis (AK) is a severe eye infection that can cause severe vision loss, especially in contact lens wearers. Rapid and accurate diagnosis is crucial for achieving favorable outcomes. Traditional methods, such as culture, are slow and have
Sarah Khaksar, Alireza Latifi, Mehdi Mohebali, Mehrnaz Atighehchian, Asad Moradian, Hassan Asadigandomani, Mostafa Rezaian, Narges Anasori, Mona Koosha, Kyle Pellegrino, Shahrokh Izadi, Fatemeh Goudarzi, Elham Kazemirad, Mohammad Soleimani   +13 more
doaj   +2 more sources

Development and Evaluation of a Dual-Target One-Step Nested PCR for the Detection of Spotted Fever Group Rickettsia spp. in Ticks [PDF]

Pathogens
Spotted fever group (SFG) rickettsioses are tick-borne infectious diseases caused by more than 30 Rickettsia species. As ticks may harbor and transmit multiple pathogens during a single blood meal, sensitive and specific molecular detection methods are ...
Phiaw Chong Foo, Canedy Jacob, Christina Injan Mawang, Ernieenor Faraliana Che Lah, Mariana Ahamad   +4 more
doaj   +2 more sources

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Demas, Allison, Oberstaller, Jenna, DeBarry, Jeremy, Lucchi, Naomi W, Srinivasamoorthy, Ganesh, Sumari, Deborah, Kabanywanyi, Abdunoor M, Villegas, Leopoldo, Escalante, Ananias A, Kachur, S Patrick, Barnwell, John W, Peterson, David S, Udhayakumar, Venkatachalam, Kissinger, Jessica C   +13 more
core   +1 more source

Application of a Nested, Multiplex PCR to Psittacosis Outbreaks [PDF]

Journal of Clinical Microbiology, 1997
We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene.
T O, Messmer, S K, Skelton, J F, Moroney, H, Daugharty, B S, Fields   +4 more
openaire   +3 more sources

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases, 2011
Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc, Thaís da Rocha Boeira, MSc, André Salvador Kazantzi Fonseca, MSc, Nilo Ikuta, PhD, Vagner Ricardo Lunge, PhD   +4 more
doaj   +1 more source

Comparison of loop-mediated isothermal amplification, polymerase chain reaction, and selective isolation assays for detection of Xanthomonas albilineans from sugarcane [PDF]

, 2018
A loop-mediated isothermal amplification (LAMP) assay was developed and compared to polymerase chain reaction (PCR), nested PCR, and selective isolation assays for detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald.
Comstock, Jack C., Cunha, Marcos G., Duarte Dias, Vanessa, Fernandez, Emmanuel, Hincapie, Martha, Pieretti, Isabelle, Rott, Philippe, Roumagnac, Philippe   +7 more
core   +2 more sources

An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

Revista da Sociedade Brasileira de Medicina Tropical, 2013
Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fabio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson Jose Venancio   +6 more
doaj   +1 more source

One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples

Frontiers in Veterinary Science, 2020
Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang, Joong Ki Song, Seongho Shin, Ki Myung Choi, Hyunil Kim   +4 more
doaj   +1 more source

Prevalência da infecção por Ehrlichia em cães e carrapatos no Nordeste do Brasil [PDF]

, 2010
This study investigated the epidemiology of canine ehrlichiosis in Northeastern Brazil, focusing the identification of the Ehrlichia species and vectors involved.
ALCÂNTARA, Adriano Costa De, BARBOZA, Débora Cristina Portela Medina, FERREIRA, Fernando, FRANKE, Carlos Roberto, GONDIM, Luís Fernando Pita, LABRUNA, Marcelo Bahia, LEAL, Danielle Custódio, SOUZA, Bárbara Maria Paraná da Silva, UZÊDA, Rosângela Soares   +8 more
core   +2 more sources

Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]

Journal of Clinical Microbiology, 1998
ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F., Slater, Kim, Owens, Jessica H., Nicholson, William L., Mather, Thomas N., Solberg, Victoria B., Olson, James G.   +6 more
openaire   +2 more sources