INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi +5 more
doaj +1 more source
The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations [PDF]
Neospora caninum and Toxoplasma gondii are closely related intracellular protozoan parasites associated with bovine and ovine abortion respectively. Little is known about the extent of Neospora/Toxoplasma co-infection in naturally infected populations of
Cook, DAN +7 more
core +1 more source
Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa +8 more
doaj +1 more source
Attempts to detect retrotransposition and de novo deletion of Alus and other dispersed repeats at specific loci in the human genome [PDF]
Dispersed repeat elements contribute to genome instability by de novo insertion and unequal recombination between repeats. To study the dynamics of these processes, we have developed single DNA molecule approaches to detect de novo insertions at a single
AJ Jeffreys +14 more
core +1 more source
Nested PCR Analysis of Plasmodium Parasites [PDF]
In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire +2 more sources
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria +5 more
doaj +1 more source
Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae [PDF]
Dermanyssus gallinae is a haematophagous ectoparasite responsible for anemia, weight loss, dermatitis and a decrease in egg production. Dermanyssus gallinae may play a role in the modulation of the host immune system, maybe predisposing the host to some ...
Cafiero, Maria Assunta +5 more
core +3 more sources
Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi +3 more
doaj +1 more source
Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]
The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA +2 more
doaj +1 more source
An unusual presentation of a case of human psittacosis [PDF]
Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina +7 more
core +3 more sources

