Results 21 to 30 of about 982,945 (306)

Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]

Journal of Clinical Microbiology, 1998
ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F., Slater, Kim, Owens, Jessica H., Nicholson, William L., Mather, Thomas N., Solberg, Victoria B., Olson, James G.   +6 more
openaire   +2 more sources

Nested PCR Analysis of Plasmodium Parasites [PDF]

, 2003
In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire   +2 more sources

Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

Kufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi, Baqur A. Sultan, Muhsin A. Al-Dhalimi, Thikra A. Mahmood   +3 more
doaj   +1 more source

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj   +1 more source

COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]

Acta Medica Iranica, 1997
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1, A.A. Velayati, M. Bahadori, M. Masjedi   +3 more
doaj   +2 more sources

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

PCR analysis of the presence and location of Mycobacterium avium in a constructed reed bed, with implications for avian tuberculosis control [PDF]

, 2009
The potential of reed beds to act as biofilters of pathogenic and environmental mycobacteria was investigated through examination of the fate of mycobacteria in a constructed reed bed filtering effluent from a large captive wildfowl collection ...
ZSL, Mwangi, D, Donoghue, H D, Wildfowl And Wetlands Trust, Cromie, RL, Drewe, JA, Donoghue, HD, Drewe, J A, Cromie, R L, UCL   +9 more
core   +1 more source

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

BMC Infectious Diseases, 2004
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria, Giuliani Giuseppe, De Vecchi Elena, Lombardi Alessandra, Drago Lorenzo, Gismondo Maria   +5 more
doaj   +1 more source

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source

Evaluation of Nested PCR in Diagnosis of Typhoid Fever [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal ...
Pradyot, Prakash, Om Prakash, Mishra, Alok Kumar, Singh, Anil Kumar, Gulati, Gopal, Nath   +4 more
openaire   +2 more sources