Results 21 to 30 of about 188,318 (309)

A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

open access: yesRevista da Sociedade Brasileira de Medicina Tropical, 2010
INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi   +5 more
doaj   +1 more source

The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations [PDF]

open access: yes, 2006
Neospora caninum and Toxoplasma gondii are closely related intracellular protozoan parasites associated with bovine and ovine abortion respectively. Little is known about the extent of Neospora/Toxoplasma co-infection in naturally infected populations of
Cook, DAN   +7 more
core   +1 more source

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

open access: yesArquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa   +8 more
doaj   +1 more source

Attempts to detect retrotransposition and de novo deletion of Alus and other dispersed repeats at specific loci in the human genome [PDF]

open access: yes, 2001
Dispersed repeat elements contribute to genome instability by de novo insertion and unequal recombination between repeats. To study the dynamics of these processes, we have developed single DNA molecule approaches to detect de novo insertions at a single
AJ Jeffreys   +14 more
core   +1 more source

Nested PCR Analysis of Plasmodium Parasites [PDF]

open access: yes, 2003
In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire   +2 more sources

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

open access: yesBMC Infectious Diseases, 2004
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria   +5 more
doaj   +1 more source

Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae [PDF]

open access: yes, 2011
Dermanyssus gallinae is a haematophagous ectoparasite responsible for anemia, weight loss, dermatitis and a decrease in egg production. Dermanyssus gallinae may play a role in the modulation of the host immune system, maybe predisposing the host to some ...
Cafiero, Maria Assunta   +5 more
core   +3 more sources

Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

open access: yesKufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi   +3 more
doaj   +1 more source

Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]

open access: yesKafkas Universitesi Veteriner Fakültesi Dergisi, 2022
The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA   +2 more
doaj   +1 more source

An unusual presentation of a case of human psittacosis [PDF]

open access: yes, 2018
Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina   +7 more
core   +3 more sources

Home - About - Disclaimer - Privacy