Nested-PCR for detection of Brucella in milk
To detect Brucella inflection in milk, a nested-PCR assay was developed by designing outward-directed and inward-directed primers for omp25, omp31 and 16S rRNA genes of Brucella abortus, and optimizing the extraction of Brucella genomic DNA. The results show that two rounds of amplification with the two pairs of synthesized primers F4/R2 and F2D/R1U ...
DU Zhen-kun +4 more
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Development and application of an indirect ELISA and nested PCR for the epidemiological analysis of Klebsiella pneumoniae among pigs in China. [PDF]
Wu Z +17 more
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Comparative Evaluation of LAMP and Nested PCR for the Rapid Diagnosis of Mycobacterium marinum Infection. [PDF]
Feng Y +5 more
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Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study. [PDF]
Shaw AG +24 more
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A nested PCR-based point-of-care multiplex test for detection of bacterial pathogens in cerebrospinal fluids. [PDF]
Wu J +17 more
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New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method. [PDF]
Aziz Anah S, Aziz Anah S.
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UPrimer: A Clade-Specific Primer Design Program Based on Nested-PCR Strategy and Its Applications in Amplicon Capture Phylogenomics. [PDF]
Li J, Han G, Tian X, Liang D, Zhang P.
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Optimization of Nested-PCR for the detection of <i>Plasmodium malariae</i> in laboratory settings in Southern Vietnam. [PDF]
Nguyen KT +6 more
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Detection of Epstein-Barr virus infection in thymic epithelial tumors by nested PCR and Epstein-Barr-encoded RNA ISH. [PDF]
Zhao L, Ding JY, Tao YL, Zhu K, Chen G.
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