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Expression of recombinant green fluorescent protein in Bacillus methanolicus
Biotechnology Progress, 2012AbstractMicrobial biocatalysts are used in a wide range of industries to produce large scale quantities of proteins, amino acids, and commodity chemicals. While the majority of these processes use glucose or other low‐cost sugars as the substrate, Bacillus methanolicus is one example of a biocatalyst that has shown sustained growth on methanol as a ...
Dewi, Nilasari +3 more
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Bacillus methanolicus: an emerging chassis for low-carbon biomanufacturing
Trends in BiotechnologyBacillus methanolicus is a thermophilic methylotrophic bacterium that grows quickly on methanol in sea water-based media. It has been engineered for chemical bioproduction from methanol, but its efficiency needs improvement for industrialization. Synthetic biology approaches such as metabolic modeling and genome editing can reprogram B.
Xueting Yang, Zhaojuan Zheng, Yu Wang
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Microbiology, 2013
The thermotolerant Gram-positive methylotroph Bacillus methanolicus is able to grow with methanol, glucose or mannitol as a sole carbon and energy source. Fructose 1,6-bisphosphate aldolase (FBA), a key enzyme of glycolysis and gluconeogenesis, is encoded in the genome of B.
Stolzenberger, Jessica +2 more
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The thermotolerant Gram-positive methylotroph Bacillus methanolicus is able to grow with methanol, glucose or mannitol as a sole carbon and energy source. Fructose 1,6-bisphosphate aldolase (FBA), a key enzyme of glycolysis and gluconeogenesis, is encoded in the genome of B.
Stolzenberger, Jessica +2 more
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Journal of Biotechnology, 2014
Bacillus methanolicus MGA3 was isolated from freshwater marsh soil and characterised as a thermotolerant and methylotrophic L-glutamate producer. The complete genome consists of a circular chromosome and the two plasmids pBM19 and pBM69. It includes genomic information about C1 metabolism and amino acid biosynthetic pathways.
Irla, Marta +8 more
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Bacillus methanolicus MGA3 was isolated from freshwater marsh soil and characterised as a thermotolerant and methylotrophic L-glutamate producer. The complete genome consists of a circular chromosome and the two plasmids pBM19 and pBM69. It includes genomic information about C1 metabolism and amino acid biosynthetic pathways.
Irla, Marta +8 more
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Proteomic analysis of the thermophilic methylotroph
Bacillus methanolicus MGA3 is a facultative methylotroph of industrial relevance that is able to grow on methanol as its sole source of carbon and energy. The Gram‐positive bacterium possesses a soluble NAD+‐dependent methanol dehydrogenase and assimilates formaldehyde via the ribulose monophosphate (RuMP) cycle.
Müller, Jonas E N +6 more
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Applied Microbiology and Biotechnology, 2014
Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value ...
Müller, Jonas E. N. +4 more
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Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value ...
Müller, Jonas E. N. +4 more
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Journal of Biotechnology, 2017
Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical mutant and recombinant strains.
Nærdal, Ingemar +6 more
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Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical mutant and recombinant strains.
Nærdal, Ingemar +6 more
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Industrial Crops and Products, 2017
Abstract The use of methanol as a carbon source for biotechnological processes has recently attracted great interest due to relatively low price, high abundance, high purity of methanol, and the fact that it is a non-food raw material. In this study, methanol-based production of γ-aminobutyric acid (GABA), which is a component of drugs and functional
Irla, Marta +3 more
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Abstract The use of methanol as a carbon source for biotechnological processes has recently attracted great interest due to relatively low price, high abundance, high purity of methanol, and the fact that it is a non-food raw material. In this study, methanol-based production of γ-aminobutyric acid (GABA), which is a component of drugs and functional
Irla, Marta +3 more
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Metabolic Engineering
Methanol-based biomanufacturing holds great promise for sustainability but is currently limited by the slow growth and low efficiency of natural or synthetic methylotrophic strains. In contrast, the thermophilic methylotroph Bacillus methanolicus exhibits rapid growth, high-temperatures tolerance, and efficient methanol utilization in defined mineral ...
Bixiao, Li +5 more
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Methanol-based biomanufacturing holds great promise for sustainability but is currently limited by the slow growth and low efficiency of natural or synthetic methylotrophic strains. In contrast, the thermophilic methylotroph Bacillus methanolicus exhibits rapid growth, high-temperatures tolerance, and efficient methanol utilization in defined mineral ...
Bixiao, Li +5 more
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Expression of Recombinant Green Fluorescent Protein in Bacillus methanolicus
2019openaire +1 more source