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Sporulation of Bacillus subtilis

Current Opinion in Microbiology, 2004
Differentiation of vegetative Bacillus subtilis into heat resistant spores is initiated by the activation of the key transcription regulator Spo0A through the phosphorelay. Subsequent events depend on the cell compartment-specific action of a series of RNA polymerase sigma factors.
Patrick J, Piggot, David W, Hilbert
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Cytochromes of Bacillus megaterium and Bacillus subtilis

Archives of Biochemistry and Biophysics, 1960
Abstract Treatment of Bacillus subtilis and Bacillus megaterium cells by ordinary procedures failed to extract any soluble cytochrome from the cell. Sonoration produced small fragments which contained the cytochrome complement and were sedimentable in a centrifugal field of 144,000 × g .
L P, VERNON, J H, MANGUM
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Transformation of Bacillus subtilis

2014
Bacillus subtilis has tremendous applications in both academic research and industrial production. However, molecular cloning and transformation of B. subtilis are not as easy as those of Escherichia coli. Here we developed a simple protocol based on super-competent cells prepared from the recombinant B.
Xiao-Zhou, Zhang   +2 more
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Pyruvate kinase of Bacillus subtilis

Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Abstract Conditions are reported which stabilize pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) activity in extracts of Bacillus subtilis. These conditions include high protein concentrations and the presence of KCl and phosphoenolpyruvate.
M, Diesterhaft, E, Freese
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Autoradiography of the Bacillus subtilis chromosome

Journal of Molecular Biology, 1966
The DNA content of the Bacillus subtilis spore has been redetermined and found to agree with the previous estimate by Fitz-James & Young (1959) . This sets an upper limit of 3·0 × 10 9 daltons (1700 μ of the B form) as the amount of DNA in the B. subtilis genome. By autoradiography continuous chromosomal lengths of 800 to 900 μ are shown to occur
E S, Dennis, R G, Wake
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Ultrastructural cytochemistry of Bacillus subtilis

Journal of Ultrastructure Research, 1967
The ultrastructure of B. subtilis has been studied with a new procedure of fixation with glutaraldehyde and embedding in glycol-methacrylate (GMA) because cytochemical studies can be carried out directly on the ultrathin sections. The cell wall appears triple-layered with two dense layers enclosing a less dense one.
P, Granboulan, E H, Leduc
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Chemotactic repellents of Bacillus subtilis

Journal of Molecular Biology, 1976
A number of membrane-active agents, including uncouplers of oxidative phosphorylation, a permeant anion, and local anaesthetics, are repellents of Bacillus subtilis . These bacteria normally swim, but tumble occasionally. However, when given such a reagent, they tumble and then later resume the original frequency of swimming and tumbling (i.e they ...
G W, Ordal, D J, Goldman
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BACILLUS SUBTILIS 2SP BACILLUS SUBTILIS 5SP

Приоритетные направления повышения эффективности, конкурентоспособности и устойчивости аграрной отрасли
Исследована холодоустойчивость штаммов Bacillus subtilis 2СП и Bacillus subtilis 5СП , для оценки их потенциала в качестве основы ферментно-пробиотической кормовой добавки в табунном коневодстве. Штамм Bacillus subtilis 5СП продемонстрировал стабильный рост при 10 °C через 72 ч и интенсивный рост при 15 °C уже через 24 ч, что свидетельствует о высокой ...
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Biological Properties Bacillus Subtilis*

Nature, 1946
B. SUBTILIS secretes an amylosucrase (B.H. fructosidase), a proteinase which liquifies gelatine or coagulated serum, and a penicillinase. Moreover, it has marked antimicrobic properties. Nicolle, in 1907, first demonstrated its bacteriolytic powers, most pronounced for the pneumococcus, less so for B. mallei, B. pestis, B. typhosus, B.
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