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Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis.
Alimentary pharmacology & therapeutics, 2011Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP).To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis.We ...
Soriano, G +10 more
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Bacterial DNA Methylation and Methylomes
2016Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
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DNA PROCESSING REACTIONS IN BACTERIAL CONJUGATION
Annual Review of Biochemistry, 1995Bacterial conjugation is an important source of genetic plasticity. The initiation complex for conjugative transfer of transmissible plasmids—the relaxosome—is a specific DNA-protein structure that has been isolated from cells and reconstituted from purified components in vitro.
E, Lanka, B M, Wilkins
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DNA repair by bacterial AlkB proteins
Research in Microbiology, 2003The Escherichia coli AlkB protein is an iron- and 2-oxoglutarate-dependent oxygenase, repairing 1-methyladenine and 3-methylcytosine lesions in DNA. AlkB homologues are present in a number of bacterial species, and some bacteria have two different AlkB proteins.
Pål Ø, Falnes, Torbjørn, Rognes
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Macrophages ingest and are activated by bacterial DNA
The Journal of Immunology, 1996Abstract Recent evidence suggests that bacterial DNA activates immune responses. Here we showed that TNF-α mRNA was induced in bone marrow-derived macrophages and the macrophage cell line RAW 264 by plasmid DNA, but not by DNaseI-digested plasmid, plasmid methylated on CpG dinucleotides, or by vertebrate genomic DNA, which is ...
Stacey, K. J., Sweet, M. J., Hume, D. A.
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The Antigenicity of Bacterial DNA
2002DNA is a complex macromolecule whose immunological properties vary with base sequence and backbone structure. Although now recognized as important for normal immunity, the antigenic and immunogenic properties of DNA were originally conceptualized entirely in the context of systemic lupus erythematosus (SLE).
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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Hybridization of Bacterial DNA on Filters
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Bacterial topoisomerases and the control of DNA supercoiling
Trends in Genetics, 1990DNA in bacterial cells is under negative superhelical tension, a feature that facilitates many of the activities of DNA. Supercoiling is introduced enzymatically by DNA gyrase, and the accumulation of excessively high levels is prevented by the relaxing activity of DNA topoisomerase I.
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Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions
Ca-A Cancer Journal for Clinicians, 2021Heather Parsons +2 more
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