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DNA extraction and polymerase chain reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient quantity. DNA can be amplified with the help of PCR. The basic principle and different variants of PCR
N. Gupta
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Proceedings of the West Virginia Academy of ScienceKATIE LONG and MARK FLOOD, Forensic Science Program, Fairmont State University, Fairmont, WV 26554. Evaluation of DNA Extraction Methods. Three forms of DNA extraction types include solution-based, column-based, and magnetic bead-based methodologies, with the latter being the accepted methodology used in forensic laboratories. Magnetic bead-based DNA
Katie Long
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Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing [PDF]
Southern African Journal of Infectious DiseasesThis study evaluated six DNA (Deoxyribonucleic acid) extraction methods for Mycobacterium avium, focusing on cost, procedure time, yield, quality, DNA integrity, and suitability for amplicon-based sequencing.
Christoffel J. Opperman +8 more
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Isolation and Characterization of Squalene Synthase Gene in Three Species of Achillea, a Rich Source of Saponins [PDF]
Journal of Genetic Resources, 2022 Squalene synthase (SQS, EC.2.5.1.21) is a key enzyme involved in the biosynthesis pathway of triterpenoid and steroidal saponins. The present study aimed to collect molecular information about the SQS gene in Achillea species, the medicinal plants rich ...
Azra Saboora +3 more
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Applications in Plant Sciences, 2023 Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization.
John J. Schenk +3 more
semanticscholar +1 more source
Molecular Ecology Resources, 2021 Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology.
N. Straube +10 more
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Science of the Total Environment, 2021 Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for identification of whole communities within a targeted group) is revolutionizing the field of aquatic biomonitoring.
J. Pawlowski +34 more
semanticscholar +1 more source
The quality and quantity of compounds affected by viral inactivation methods in dried blood spots
Journal of Laboratory Medicine, 2023 The aim is to evaluate the effect of viral inactivation methods on the quality and quantity of compounds in dried blood spots (DBS).
Wang Ming +5 more
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The American Biology Teacher, 2022 The Next Generation Science Standards (NGSS) were proposed in 2013 in the United States for K–12 learning to emphasize the importance of science and engineering practices in the three dimensions of science learning. In this study, we show that DNA extraction experiments, which are familiar in the United States, can be used not only as demonstration ...
Naoko Kosaka, Yoshisuke Kumano
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Macedonian Veterinary Review, 2020 The probability of contamination of non-transgenic varieties with genetically modified (GM) products increase as a result of global expansion of areas sown with transgenic crops.
Arita Sabriu-Haxhijaha +3 more
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