Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool [PDF]
PLoS Neglected Tropical Diseases, 2019 Background A DNA extraction and preservation protocol that yields sufficient and qualitative DNA is pivotal for the success of any nucleic acid amplification test (NAAT), but it still poses a challenge for soil-transmitted helminths (STHs), including ...
Mio Ayana +9 more
semanticscholar +3 more sources
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]
, 2020 Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet +3 more
core +1 more source
The Effects of Freezing on Faecal Microbiota as Determined Using MiSeq Sequencing and Culture-Based Investigations [PDF]
, 2015 peer-reviewedBackground High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota.
Deane, Jennifer +7 more
core +5 more sources
Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran [PDF]
Archives of Razi Institute, 2020 Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran.
S. Zarrabi Ahrabi +4 more
doaj +1 more source
The Evolution of DNA Extraction Methods
American Journal of Biomedical Science & Research, 2020 Since the first DNA extraction performed by Friedrich Miescher in 1869, scientists have made extraordinary progress in designing extraction methods that are more reliable, easier and faster to perform, more cost-effective and produce a higher yield.
P. Shetty
semanticscholar +1 more source
Journal of Pure and Applied Microbiology, 2022 Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp.
Narumon Tangthirasunun, Supattra Poeaim
doaj +1 more source
Assessment of fecal DNA extraction protocols for metagenomic studies
GigaScience, 2020 Background Shotgun metagenomic sequencing has improved our understanding of the human gut microbiota. Various DNA extraction methods have been compared to find protocols that robustly and most accurately reflect the original microbial community ...
Fangming Yang +13 more
semanticscholar +1 more source
Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements [PDF]
, 2010 In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless,
Martin, I., Piccinini, E., Sadr, N.
core +1 more source
Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers.
Marzieh Rabbani +1 more
doaj +1 more source
Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability [PDF]
, 2013 Background: The need for new malaria surveillance tools and strategies is critical, given improved global malaria control and regional elimination efforts.
Abdullah S Ali +26 more
core +2 more sources