Results 41 to 50 of about 5,294,356 (259)

DNA extraction for human microbiome studies: the issue of standardization

Genome Biology, 2019
Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions.
K. Greathouse, R. Sinha, E. Vogtmann
semanticscholar   +1 more source

Evaluation of pre-analytical factors affecting plasma DNA analysis. [PDF]

, 2018
Pre-analytical factors can significantly affect circulating cell-free DNA (cfDNA) analysis. However, there are few robust methods to rapidly assess sample quality and the impact of pre-analytical processing. To address this gap and to evaluate effects of
Berens, Michael E, Borad, Mitesh J, Bryce, Alan, Contente-Cuomo, Tania, Dhruv, Harshil D, Farooq, Maria, Gollins, Simon, Liang, Winnie S, LoRusso, Patricia M, Markus, Havell, Murtaza, Muhammed, Ribas, Antoni, Sekulic, Aleksandar, Sivakumar, Shivan, Tran, Nhan L, Trent, Jeffrey M   +15 more
core   +1 more source

Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

Nature Protocols, 2020
Short-read metagenomic sequencing and de novo genome assembly of the human gut microbiome can yield draft bacterial genomes without isolation and culture. However, bacterial genomes assembled from short-read sequencing are often fragmented.
Dylan G. Maghini, Eli Moss, Summer E. Vance, A. Bhatt   +3 more
semanticscholar   +1 more source

Using eggshell membranes as a DNA source for population genetic research [PDF]

, 2009
In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers.
Broekman, J., Kentie, R., Musters, C.J.M., Snoo, G.R., de, Trimbos, K.B.   +4 more
core   +3 more sources

Comparison of DNA extraction methods for molecular identification of pathogenic Leptospira in the urine samples

Health Science Journal of Indonesia, 2020
Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat ...
Farida Dwi Handayani, Rahmi Ayu Wijayaningsih, Muhammad Hussein Gasem, Tri Wibawa   +3 more
doaj   +1 more source

Effects of sampling strategy and DNA extraction on human skin microbiome investigations

Scientific Reports, 2019
The human skin is colonized by a wide array of microorganisms playing a role in skin disorders. Studying the skin microbiome provides unique obstacles such as low microbial biomass.
R. Bjerre, L. Hugerth, Fredrik Boulund, M. Seifert, J. Johansen, L. Engstrand   +5 more
semanticscholar   +1 more source

Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays [PDF]

, 2018
Background Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols ...
Abras, Alba, Ballart, Cristina, Berenguer, Pere, Gascón, Joaquim, Gutiérrez, Cristina, Gállego, Montserrat, Llovet, Teresa, Muñoz, Carmen, Pinazo, María-Jesús, Posada, Elizabeth, Roig, Carme, Schijman, Alejandro Gabriel, Tebar, Silvia   +12 more
core   +3 more sources

Application of a multiplex polymerase chain reaction (mPCR) assay to detect fraud by substitution of bovine meat cuts with water buffalo meat in Northern Brazil

CyTA - Journal of Food, 2019
The adulteration of meat products can affect the confidence of consumers and the market, leading to negative impacts on the economy. Accordingly, product authenticity has become an important issue in modern society. Therefore, our study aimed to optimize
Vanderson Vasconcelos Dantas, Gabrielle Virginia Ferreira Cardoso, Wanessa Shuelen Costa Araújo, Andrey Carlos do Sacramento de Oliveira, Andreia Silva da Silva, Josyane Brasil da Silva, Silvia Cristina da Silva Pedroso, Talita Bandeira Roos, Carina Martins de Moraes, Lúcia de Fátima Henriques Lourenço   +9 more
doaj   +1 more source

Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

mSystems, 2020
Method choice throughout the workflow of a microbiome study, from sample collection to DNA extraction and sequencing procedures, can greatly affect results.
Q. R. Ducarmon, B. Hornung, A. R. Geelen, E. Kuijper, R. Zwittink   +4 more
semanticscholar   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A., Bahkali, A.H., Moslem, M.A., Wit, P.J.G.M., de   +3 more
core   +2 more sources