Results 41 to 50 of about 939,296 (267)

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2014
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

DNA extraction for HMW DNA v1 [PDF]

open access: yes, 2019
This protocol is intended for extraction of HMW DNA from bacterial or viral samples.
Natalie Solonenko, Marie Burris
openaire   +1 more source

Comparative Study of Extracted Nucleic Acid from Escherichia coli by Two Methods Phenol-chloroform and Extraction Using Magnetic Nanoparticle

open access: yesBihdād, 2020
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least ...
Ala Amiri   +3 more
doaj  

Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time

open access: yesIndonesian Journal of Halal Research, 2023
DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo   +5 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

open access: yesFEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg   +14 more
wiley   +1 more source

Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 inbanana

open access: yesBiotecnología Vegetal, 2021
Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen.
María I. Oloriz   +4 more
doaj  

Determining the optimal method for DNA isolation from fruit jams

open access: yesCzech Journal of Food Sciences, 2018
DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances.
Tereza SOVOVÁ   +2 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

open access: yesFEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas   +4 more
wiley   +1 more source

Investigation of Bm86 gene analog sequence in some Iranian populations of Hyalomma anatolicum species [PDF]

open access: yesIranian Journal of Animal Biosystematics, 2006
Ticks are an important problem among zoonotic diseases. Main veterinary species of hard ticks are: Hyalomma sp. Boophilus sp and Rhipicephalus sp. Among them Hyalomma sp. have widespread distribution in Iran and Middle East.
S. Abdollahi   +2 more
doaj   +1 more source

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