A minimally destructive protocol for DNA extraction from ancient teeth
bioRxiv, 2020 Ancient DNA sampling methods—although optimized for efficient DNA extraction—are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive
Éadaoin Harney +40 more
semanticscholar +1 more source
Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies. [PDF]
, 2013 BACKGROUND: Informing and evaluating malaria control efforts relies on knowledge of local transmission dynamics. Serological and molecular tools have demonstrated great sensitivity to quantify transmission intensity in low endemic settings where the ...
Baidjoe, Amrish +13 more
core +3 more sources
Overcoming DNA extraction problems from carnivorous plants
Anales del Jardín Botánico de Madrid, 2009 We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants.
Andreas Fleischmann, Günther Heubl
doaj +1 more source
Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers [PDF]
Journal of Genetic Resources, 2020 Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants.
Samira Abolghasemi +2 more
doaj +1 more source
Nucleic Acids Research, 1997 A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is furthermore strongly promoted by submillimolar phosphate anion concentrations.
E, Kejnovský, J, Kypr
openaire +3 more sources
Using microbiome information to understand and improve animal performance
Italian Journal of Animal Science, 2022 For many years, microbiology was based on culture-dependent methods to study microorganisms; however, the latest technologies are culture-independent and their development has generated large amounts of new data, allowing researchers to understand the ...
Jeferson Menezes Lourenco +1 more
doaj +1 more source
DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
Scientific Reports, 2020 In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant.
C. Douglas +5 more
semanticscholar +1 more source
Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]
, 2017 Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K. +6 more
core +2 more sources
Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR
Safety, 2019 Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat ...
Lavinia-Maria Chiş +1 more
doaj +1 more source
Qualitative PCR-based detection of genetically modified soy and maize products in Iran
International Journal of Food Properties, 2020 Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to ...
Payam Safaei +5 more
doaj +1 more source