Results 21 to 30 of about 939,296 (267)

Comparison of DNA extraction methods for molecular identification of pathogenic Leptospira in the urine samples

open access: yesHealth Science Journal of Indonesia, 2020
Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat ...
Farida Dwi Handayani   +3 more
doaj   +1 more source

Optimization of DNA extraction for insect museomics substantially increases DNA yield

open access: yesEuropean Journal of Entomology
Historical samples from museum and private collections can serve as a time machine, allowing us to follow changes in genetic composition through time as well as obtaining genomic data on past biodiversity.
Marie DJERNÆS   +8 more
doaj   +1 more source

Modified Phenol-Chloroform Method for Dermatophyte DNA Extraction: Optimization of Culture Media and Extraction Steps to Improve Quality and Quantity of Fungal DNA

open access: yesJournal of Pure and Applied Microbiology
Complex procedures are required for DNA extraction from filamentous fungi owing to the structural complexity of their cell walls. Disruption of fungal cells results in the release of large amounts of polysaccharides and proteins.
A. Jain Pavithra   +4 more
doaj   +1 more source

Comparison of Lysis Buffers for Saliva DNA Extraction using Modified Magnetic-Silica Nanoparticle Beads

open access: yesBiotropika: Journal of Tropical Biology, 2023
Our previous study revealed that modified MAGSi (magnetic-silica nanopartivel beads) had various sizes and shapes that affected the DNA extraction process.
Muhammad Fauzi Arif   +3 more
doaj   +1 more source

Organoids in pediatric cancer research

open access: yesFEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

A simple genetic method to distinguish mule deer and bighorn sheep fecal pellets and its application to detecting bighorn sheep colonization events in California

open access: yesCalifornia Fish and Wildlife Journal, 2023
Bighorn sheep (Ovis canadensis) habitat frequently is geographically discontinuous and the metapopulation model fits this species well. Consequently, extinction-colonization dynamics are important and need to be monitored.
John Wehausen
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

Quality of DNA Extracted from Mouthwashes [PDF]

open access: yesPLoS ONE, 2009
A cost effective, safe and efficient method of obtaining DNA samples is essential in large scale genetic analyses. Buccal cells are an attractive source of DNA, as their collection is non-invasive and can be carried out by mail. However, little attention has been given to the quality of DNA extracted from mouthwashes.Mouthwash-derived DNA was extracted
Zayats, T   +5 more
openaire   +5 more sources

The human gut microbiome across the life course

open access: yesFEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero   +4 more
wiley   +1 more source

Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification

open access: yesJournal of Forensic Science and Medicine
Background: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato   +5 more
doaj   +1 more source

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