An efficient protocol to perform genetic traceability of tissue and foods from Geoffroea decorticans
The quality of a DNA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans Burkart (chanar) is a species that has nutritional and pharmacological potential.
Aguayo, F. +3 more
core +1 more source
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini +3 more
core +1 more source
Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification
Background: Nails are one of the objects that are more durable than other witness objects. This study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory results.
Kanchana Sujirachato +5 more
doaj +1 more source
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of oral microbiota. However, it remains not clear whether and to what degree DNA extraction and targeted 16S rRNA hypervariable regions influence the analysis ...
F. Teng +7 more
semanticscholar +1 more source
Following basal stem rot in young oil palm plantings [PDF]
The PCR primer GanET has previously been shown to be suitable for the specific amplification of DNA from Ganoderma boninense. A DNA extraction and PCR method has been developed that allows for the amplification of the G.
CA Levesque +17 more
core +1 more source
Inter-DNA Attraction Mediated by Divalent Counterions
Can nonspecifically bound divalent counterions induce attraction between DNA strands? Here, we present experimental evidence demonstrating attraction between short DNA strands mediated by Mg2 ions.
Andresen, Kurt +5 more
core +1 more source
Rapid plant DNA and RNA extraction protocol using a bench drill [PDF]
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after.
Amorim, Edson Perito +11 more
core +3 more sources
Robust CTAB-activated charcoal protocol for plant DNA extraction
DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction.
Mitja KRIŽMAN +4 more
doaj +1 more source
Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR [PDF]
Introduction: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method.
Mahmoud Mohammed Sirdah
doaj +1 more source
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time
DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins.
Hadi Sunaryo +5 more
doaj +1 more source

