DNA extraction for streamlined metagenomics of diverse environmental samples.
BioTechniques, 2017 A major bottleneck for metagenomic sequencing is rapid and efficient DNA extraction. Here, we compare the extraction efficiencies of three magnetic bead-based platforms (KingFisher, epMotion, and Tecan) to a standardized column-based extraction platform ...
C. Marotz +5 more
semanticscholar +1 more source
Bihdād, 2020 Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least ...
Ala Amiri +3 more
doaj
Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 inbanana
Biotecnología Vegetal, 2021 Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen.
María I. Oloriz +4 more
doaj
Determining the optimal method for DNA isolation from fruit jams
Czech Journal of Food Sciences, 2018 DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances.
Tereza SOVOVÁ +2 more
doaj +1 more source
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
, 2016 peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
core +2 more sources
Estimating bacterial DNA synthesis from [3H] thymidine incorporation : discrepancies among macromolecular extraction procedures [PDF]
, 1991 The estimation of bacterial DNA synthesis in trophic studies with [3H] thymidine requires quantitative extraction of labeled DNA. To determine the DNA contribution to total macromolecular labeling in a eutrophic ecosystem, we tested three extraction ...
Bouvy, Marc, Torréton, Jean-Pascal
core +1 more source
ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH [PDF]
Basrah Journal of Veterinary Research, 2016 Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar ...
Sinan J. Abdul-Abbas +3 more
doaj +1 more source
Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types
Metabarcoding and Metagenomics, 2018 Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed.
G. Sellers +3 more
semanticscholar +1 more source
Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality? [PDF]
, 2016 Background: Advanced genomic techniques such as Next-Generation-Sequencing (NGS) and gene expression profiling, including NanoString, are vital for the development of personalised medicines, as they enable molecular disease classification.
A Kalmar +31 more
core +1 more source
Lysis Buffers for Detecting Pork Content in Food Products
Indonesian Journal of ChemistryThe increasing number of counterfeit processed food products with non-halal ingredients, such as pork, has caused public concern about the halalness of Indonesian food products, especially in the Muslim community.
Ulfah Lailatul Khoiriah +5 more
doaj +1 more source