Results 91 to 100 of about 5,294,356 (259)

Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota

Scientific Reports, 2019
The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS).
J. Rosenbaum, Mykhaylo Usyk, Zigui Chen, Christine P. Zolnik, H. Jones, L. Waldron, J. Dowd, L. Thorpe, R. Burk   +8 more
semanticscholar   +1 more source

Comparison and Optimization of Ancient DNA Extraction [PDF]

BioTechniques, 2007
Ancient DNA analyses rely on the extraction of the tiny amounts of DNA remaining in samples that are hundreds to tens of thousands of years old. Despite the critical role extraction efficiency plays in this field of research, no study has comprehensively compared ancient DNA extraction techniques to date.
Nadin Rohland, Michael Hofreiter
openaire   +3 more sources

A simplified DNA extraction protocol for unsorted bulk arthropod samples that maintains exoskeletal integrity

Environmental DNA, 2019
High-throughput DNA sequencing offers an efficient tool for assessing the taxonomic content of bulk arthropod samples. Many current DNA extraction protocols however require extensive handling of samples, like specimen-based DNA extractions, or sorting of
M. Nielsen, Thomas P. Gilbert, T. Pape, Kristine Bohmann   +3 more
semanticscholar   +1 more source

DNA Renaturation at the Water-Phenol Interface [PDF]

, 2003
We study DNA adsorption and renaturation in a water-phenol two-phase system, with or without shaking. In very dilute solutions, single-stranded DNA is adsorbed at the interface in a salt-dependent manner.
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core   +4 more sources

Quality of DNA Extracted from Mouthwashes [PDF]

PLoS ONE, 2009
A cost effective, safe and efficient method of obtaining DNA samples is essential in large scale genetic analyses. Buccal cells are an attractive source of DNA, as their collection is non-invasive and can be carried out by mail. However, little attention has been given to the quality of DNA extracted from mouthwashes.Mouthwash-derived DNA was extracted
Zayats, T, Young, TL, Mackey, DA, Malecaze, F, Calvas, P, Guggenheim, JA   +5 more
openaire   +5 more sources

Impacts of DNA extraction and PCR on DNA metabarcoding estimates of soil biodiversity

Methods in Ecology and Evolution, 2018
Soils are ubiquitous and important components of terrestrial ecosystems, richly populated with diverse organisms with important ecological roles. DNA metabarcoding is a promising tool for efficient and taxonomically comprehensive analyses of soil ...
A. Dopheide, Dong Xie, T. Buckley, A. Drummond, R. Newcomb   +4 more
semanticscholar   +1 more source

Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens [PDF]

, 1994
Discrimination of pathogenic and nonpathogenic Entamoeba histolytica is of great clinical importance. A simple and rapid DNA extraction method that can be used directly with stool specimens was developed without the need for prior cultivation of the ...
Katzwinkel-Wladarsch, S., Löscher, Thomas, Rinder, H.   +2 more
core   +1 more source

Whole Genome Sequencing of A Healthy Sundanese Individual As an Initial Study for Sundanese Population Genetic Reference Foundation [PDF]

BIO Web of Conferences
Sundanese is the second-largest tribe in Indonesia. Despite its substantial and diverse population, the application and database of whole-genome sequencing in Indonesia, especially among Sundanese individuals, are limited.
Jamilah Richa Wijayanti, Andrianto Dimas, Pratama Rahadian, Budhyanto Vincentius Simeon Weo, Pudjas Nabila Tsoerayya Gustia   +4 more
doaj   +1 more source

Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]

, 2010
Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed, Bakkali Yakhlefb, Salaheddine, Guenoun, Imane, Hamamouch, Noureddine, kerdouh, Benaîssa   +4 more
core   +2 more sources

DNA extraction: High yield is not synonymous with high diversity

Bioingeniøren, 2017
The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone, Vahid Bemanian, Hege Smith Tunsjø   +2 more
doaj