Narrative Review of Bacterial DNA Extraction: Key Points and Challenges [PDF]
SINAI International Scientific JournalExtraction of bacterial DNA is a critical step in obtaining high-quality DNA. The overall quality, yield, and integrity of the genomic DNA isolated affects the success of any downstream applications.
Samar Solyman, Manar El Samak
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Evaluation of plant seed DNA and botanical evidence for potential forensic applications
Forensic Sciences Research, 2020 Seeds, the reproductive organs of plants, are common as trace evidence from crime scenes. Seed evidence could be grouped into several categories based on the types of crimes they are associated with, including child abuse, homicides and drugs.
Cheng-Lung Lee +3 more
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Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?
PeerJ, 2018 Background High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring.
M. Carew, R. Coleman, A. Hoffmann
semanticscholar +1 more source
Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus [PDF]
, 2007 The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe.
BOONHAM, NEIL +9 more
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Optimization of DNA extraction from dental remains
Electrophoresis, 2019 Efficient DNA extraction procedures is a critical step involved in the process of successful DNA analysis of such samples. Various protocols have been devised for the genomic DNA extraction from human tissues and forensic stains, such as dental tissue ...
C. Cafiero +5 more
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The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review.
Journal of Food Science, 2018 High-quality DNA extracts are imperative for downstream applications in molecular identification. Most processed food products undergo heat treatments causing DNA degradation, which hampers application of DNA-based techniques for food authentication ...
N. Sajali +5 more
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A simple and rapid DNA extraction method from leaves of grapevine suitable for polymerase chain reaction analysis. [PDF]
, 2012 The genomic grapevine (Vitis vinifera L.) DNA extraction is difficult because of secondary metabolites that interfere with DNA isolation procedures and subsequent applications.
ALFONZO, Antonio +5 more
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Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus [PDF]
, 1994 Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin ...
Baker, A. +4 more
core
Monitoring genetic population biomarkers for wastewater-based epidemiology [PDF]
, 2017 We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic
Cooper, Jonathan M. +4 more
core +3 more sources
Low concentration DNA extraction and recovery using a silica solid phase
PLoS ONE, 2017 DNA extraction from clinical samples is commonly achieved with a silica solid phase extraction column in the presence of a chaotrope. Versions of these protocols have been adapted for point of care (POC) diagnostic devices in miniaturized platforms, but ...
Constantinos Katevatis +2 more
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