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Metagenomic analyses of single phages and phage cocktails show instances of contamination with temperate phages and bacterial DNA

Peng X, Smith SE, Huang W, Ru J, Mirzaei MK, Deng L.   +5 more
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Preparing Bacterial Genomic DNA

Cold Spring Harbor Protocols, 2022
We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based column in the presence of chaotropic salts (guanidine salts).
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
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Chloramphenicol damages bacterial DNA

Biochemical and Biophysical Research Communications, 1977
L(+)-threo-chloramphenicol induces reversion of His−Salmonella typhimurium strains TA100 and TA1535 in the conventional Ames' assay without microsomal activation. Any mutagenicity of D(−)-threo-chloramphenicol was masked by toxicity. Similarly, a sensitive fluctuation test showed mutagenesis with L(+)-threo-chloramphenicol at concentrations of 0.5 μM ...
S F, Jackson, B R, Wentzell, D R, McCalla, K B, Freeman   +3 more
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Bacterial DNA excision repair pathways

Nature Reviews Microbiology, 2022
Bacteria are continuously exposed to numerous endogenous and exogenous DNA-damaging agents. To maintain genome integrity and ensure cell survival, bacteria have evolved several DNA repair pathways to correct different types of DNA damage and non-canonical bases, including strand breaks, nucleotide modifications, cross-links, mismatches and ...
Katherine J. Wozniak, Lyle A. Simmons
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Comparing Bacterial DNA Microarray Fingerprints

Statistical Applications in Genetics and Molecular Biology, 2005
Epidemiologic and forensic investigations often require assays to detect subtle genetic differences between closely related microorganisms. Typically, gel electrophoresis is used to compare randomly amplified DNA fragments between microbial samples, where the patterns of DNA fragment sizes are viewed as genotype ‘fingerprints’.
Willse, Alan, Chandler, Darrell P., White, Amanda, Protic, Miroslava, Daly, Don S., Wunschel, Sharon   +5 more
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Bacterial Infection Promotes DNA Hypermethylation

Journal of Dental Research, 2007
Maternal oral infection, caused by bacteria such as C. rectus or P. gingivalis, has been implicated as a potential source of placental and fetal infection and inflammatory challenge, which increases the relative risk for pre-term delivery and growth restriction.
Y A, Bobetsis, S P, Barros, D M, Lin, J R, Weidman, D C, Dolinoy, R L, Jirtle, K A, Boggess, J D, Beck, S, Offenbacher   +8 more
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DNA transfer in bacterial conjugation

Journal of Molecular Biology, 1966
Chromosome transfer from Hfr to F − cells was analyzed by labeling the DNA of the Hfr cells with [ 3 H]thymine prior to mating, and/or by labeling any DNA made during mating. The F − cells were either starved of adenine or heavily irradiated with ultraviolet light, with the aim of preventing them from synthesizing DNA.
J D, Gross, L G, Caro
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Arrest of Bacterial DNA Replication

Annual Review of Microbiology, 1992
The chromosomes of both gram-positive and gram-negative bacteria contain sites that arrest the progression of DNA replication forks. These replication-arrest sites limit the end of the replication cycle to a particular region of the chromosome, called the terminus region.
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Bacterial CpG-DNA Licenses TLR9

2002
The family of Toll-like receptors (TLRs) plays an important role in the innate immune response to pathogens. TLRs sense pathogen associated molecular patterns (PAMP) and lead to the stimulation of immune cells. In man, so far ten members (TLR1-10) have been reported.
S, Bauer, H, Wagner
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