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Bacterial DNA Methylation and Methylomes.

Advances in Experimental Medicine and Biology, 2016
Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
J. Casadesús
semanticscholar   +4 more sources

CpG motifs in bacterial DNA and their immune effects.

Annual Review of Immunology, 2002
Unmethylated CpG motifs are prevalent in bacterial but not vertebrate genomic DNAs. Oligodeoxynucleotides (ODN) containing CpG motifs activate host defense mechanisms leading to innate and acquired immune responses. The recognition of CpG motifs requires
A. Krieg
semanticscholar   +1 more source

Preparing Bacterial Genomic DNA

Cold Spring Harbor Protocols, 2022
We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based column in the presence of chaotropic salts (guanidine salts).
Nara Figueroa-Bossi   +2 more
openaire   +2 more sources

Preparation of Bacterial Plasmid DNA

Current Protocols in Neuroscience, 1998
AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
JoAnne Engebrecht   +2 more
openaire   +4 more sources

Characterization of the bacterial microbiome in first‐pass meconium using propidium monoazide (PMA) to exclude nonviable bacterial DNA

Letters in Applied Microbiology, 2019
Numerous studies have reported bacterial DNA in first‐pass meconium samples, suggesting that the human gut microbiome is seeded prior to birth. However, these studies have not been able to discriminate between DNA from living bacterial cells, DNA from ...
L. Stinson, J. Keelan, Matthew S. Payne
semanticscholar   +1 more source

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