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Untangling bacterial DNA topoisomerases functions
Biochemical Society TransactionsTopoisomerases are the main enzymes capable of resolving the topological constraints imposed by DNA transactions such as transcription or replication. All bacteria possess topoisomerases of different types. Although bacteria with circular replicons should encounter similar DNA topology issues, the distribution of topoisomerases varies from one ...
Céline Borde +2 more
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Preparation of Bacterial Plasmid DNA
Current Protocols in Neuroscience, 1998AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
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Bacterial Dna in Mixed Cholesterol Gallstones
American Journal of Gastroenterology, 1999Numerous investigators have proposed a role for bacteria in biliary lithogenesis. We hypothesized that bacterial DNA is present in gallstones, and that categorical differences exist between gallstone type and the frequency of bacterial sequences.Polymerase chain reaction (PCR) was used to amplify bacterial 16S rRNA and uidA (encoding Escherichia coli ...
Haigh, WG, Tarr, PI, Lee, DK, Lee, SP
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Bacterial DNA strand compositional asymmetry
Trends in Microbiology, 1999Genes that deviate strongly in codon usage from the average gene but are relatively similar in codon usage to ribosomal protein (RP) genes are viewed as highly expressed22xKarlin, S., Campbell, A.M., and Mrazek, J. Annu. Rev. Genet. 1998; 32: 185–225Crossref | PubMed | Scopus (229)See all References, 23xKarlin, S., Mrazek, J., and Campbell, A.M.
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Bacterial DNA Methylation and Methylomes
2016Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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Z-form extracellular DNA is a structural component of the bacterial biofilm matrix
Cell, 2021Aishwarya Devaraj +2 more
exaly
1974
Publisher Summary This chapter discusses the isolation and physicochemical properties. Methods for isolating the bacterial polymerase share many common features. Cells are lysed by the vigorous disruptions of sonication or mechanical shear or by gentler lysozyme treatments.
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Publisher Summary This chapter discusses the isolation and physicochemical properties. Methods for isolating the bacterial polymerase share many common features. Cells are lysed by the vigorous disruptions of sonication or mechanical shear or by gentler lysozyme treatments.
openaire +1 more source