Results 61 to 70 of about 854,281 (316)

Optimizing bacterial DNA extraction in urine.

PLoS ONE, 2019
Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch, Laura C Chambers, Lisa E Manhart, Dan Domogala, Anthony Lopez, David N Fredricks, Sujatha Srinivasan   +6 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

A broad brush, global overview of bacterial sexuality [PDF]

, 2011
Bacterial sexuality is confusing, even for experts! I used to be such an expert on one mechanism of sexuality, conjugation, but that was over 30 years ago.
Mark Achtman, Achtman, Mark
core   +1 more source

Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

PLoS ONE, 2020
Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya, Tomoharu Sano, Noriko Tomioka, Ayato Kohzu, Kazuhiro Komatsu, Ryuichiro Shinohara, Shinji Shimode, Tatsuki Toda, Akio Imai   +8 more
doaj   +1 more source

Bacterial DNA topology and infectious disease [PDF]

Nucleic Acids Research, 2008
The gram-negative bacterium Escherichia coli and its close relative Salmonella enterica have made important contributions historically to our understanding of how bacteria control DNA supercoiling and of how supercoiling influences gene expression and vice versa.
Dorman, Charles J., Corcoran, Colin P.
openaire   +4 more sources

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats

, 2012
BACKGROUND: Horizontal gene transfer through natural transformation of members of the microbiota of the lower gastrointestinal tract (GIT) of mammals has not yet been described.
Raddadi Noura, Nielsen KM, Traavik T, Terje Traavik, Brusetti Lorenzo, Averhoff, Beate, Nordgård Lise, Beate Averhoff, Nordgård, Lise, Noura Raddadi, Brusetti L, Nielsen, Kaare Magne, Kaare Magne Nielsen, Traavik, Terje, Nielsen Kaare, Traavik Terje, Lorenzo Brusetti, Raddadi, Noura, Averhoff B, Brusetti, Lorenzo, Averhoff Beate, Nordgård L, Lise Nordgard   +22 more
core   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +1 more source

The emerging role of DNA methylation in the pathogenicity of bacterial pathogens

Journal of Bacteriology
Uncovering the mechanisms regulating the pathogenicity of bacterial pathogens can help improve diagnostic capabilities and aid the development of new drugs, both of which are crucial for reducing the burden caused by bacterial infections. In recent years,
Ya-xuan Ma, Xiu-dan Wang, Xin-min Li
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source