Results 51 to 60 of about 824,108 (266)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Microbiology SpectrumComprehensive whole-genome sequencing was performed on two multi-drug-resistant Escherichia coli strains isolated from cattle manure from a typical dairy farm in Poland in 2020.
Magdalena Zalewska +7 more
doaj +1 more source
BMC Microbiology, 2023 The existence of a placental microbiota is debated. The human placenta has historically been considered sterile and microbial colonization was associated with adverse pregnancy outcomes.
Jonathan J. Panzer +6 more
doaj +1 more source
Bacterial DNA amplifies neutrophilic inflammation in IL-17-exposed airways
ERJ Open Research, 2023 Background Neutrophilic asthma (NA) is associated with increased airway interleukin (IL)-17 and abnormal bacterial community such as dominance of nontypeable Haemophilus influenzae (NTHi), particularly during asthma exacerbations.
Nastaran Mues +8 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Optimizing bacterial DNA extraction in urine.
PLoS ONE, 2019 Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch +6 more
doaj +1 more source
The bacterial capsule is a gatekeeper for mobile DNA
PLOS Biology, 2021 The horizontal transfer of mobile DNA is one of the signature moves of bacterial evolution, but the specific rules that govern this transfer remain elusive. In this PLOS Biology issue, Haudiquet and colleagues revealed that the interactions between mobile genetic elements and the bacterial capsule shape the horizontal flow of DNA in an important ...
Alfonso Santos-López +2 more
openaire +5 more sources
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
The emerging role of DNA methylation in the pathogenicity of bacterial pathogens
Journal of BacteriologyUncovering the mechanisms regulating the pathogenicity of bacterial pathogens can help improve diagnostic capabilities and aid the development of new drugs, both of which are crucial for reducing the burden caused by bacterial infections. In recent years,
Ya-xuan Ma, Xiu-dan Wang, Xin-min Li
doaj +1 more source
PLoS ONE, 2020 Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya +8 more
doaj +1 more source